SG ID

SG00002079 

UniProt Accession

TXND3_RAT;Q715S9;  

Theoretical PI

4.85  

Molecular Weight

67312 Da  

Genbank Nucleotide ID

AF548544;  

Genbank Protein ID

AAQ12344.1; 

Gene Name

Nme8 

Gene Synonyms/Alias

Sptrx2, Txndc3 

Protein Name

Thioredoxin domain-containing protein 3 

Protein Synonyms/Alias

NME/NM23 family member 8; Spermatid-specific thioredoxin-2;Sptrx-2 

Rattus norvegicus (Rat) 

10116 

Temporarily unavailable 

The information of related literatures

1. A. Miranda-Vizuete, K. Tsang, Y. Yu, A. Jimenez, M. Pelto-Huikko, C. J. Flickinger, P. Sutovsky and R. Oko (2003) Cloning and developmental analysis of murid spermatid-specific thioredoxin-2 (SPTRX-2), a novel sperm fibrous sheath protein and autoantigen. J Biol Chem 278(45): 44874-85. 

Abstract
Thioredoxins compose a growing family of proteins that participate in different cellular processes via redox-mediated reactions. We report here the cloning, developmental expression, and location of murid Sptrx-2. Mouse and rat SPTRX-2 proteins display a high homology to their human ortholog in the thioredoxin and NDP kinase domains, and the coding genes are located at syntenic positions. Northern blotting and in situ hybridization confirmed the testis-specific expression of murine Sptrx-2 mRNA, mostly in round spermatids. Immunohistochemical analysis of the 19 steps of rat spermiogenesis showed that SPTRX-2 expression becomes prominent in the cytoplasmic lobe of step 15-18 spermatids and diminishes in step 19 just before spermiation. However, in the spermatid tail, SPTRX-2 immunoreactivity increased from step 15 to 19 and was confined to the principal piece. By immunogold electron microscopy, SPTRX-2 was first detected scattered throughout the cytoplasm of the axoneme in step 14-15 spermatids, but began to be incorporated by step 16 into the fibrous sheath (FS). During steps 17-18, the labeling increased over the ribs and columns of the assembled FS. It peaked in step 19 and remained in the FS of epididymal spermatozoa. Immunoblots of isolated FS obtained from spermatozoa confirmed that SPTRX-2 is an integral component of the FS and a post-obstruction autoantigen in vasectomized rats. Our data indicate that SPTRX-2 incorporation into the FS lags well behind FS assembly, suggesting it is required during the final stages of sperm tail maturation in the testis and/or epididymis, where extensive disulfide bonding of FS proteins occurs. PMID: [12909633] 

Figures for illustrating the function of this protein/gene

Function

Probably required during the final stages of sperm tailmaturation in the testis and/or epididymis, where extensivedisulfide bonding of fibrous sheath (FS) proteins occurs. May beinvolved in the reduction of disulfide bonds within the sperm FScomponents. In vitro, it has neither NDP kinase nor reducingactivity on disulfide bonds (By similarity). 

Subcellular Location

Cytoplasm. Note=In spermatozoa, it is anintegral component of the FS. 

Tissue Specificity

Testis-specific. 

Gene Ontology

GO ID	GO term	Evidence
GO:0005737	C:cytoplasm	IEA:UniProtKB-SubCell.
GO:0015630	C:microtubule cytoskeleton	IEA:Compara.
GO:0005524	F:ATP binding	IEA:InterPro.
GO:0004550	F:nucleoside diphosphate kinase activity	IEA:InterPro.
GO:0045454	P:cell redox homeostasis	IEA:InterPro.
GO:0006241	P:CTP biosynthetic process	IEA:InterPro.
GO:0006183	P:GTP biosynthetic process	IEA:InterPro.
GO:0007275	P:multicellular organismal development	IEA:UniProtKB-KW.
GO:0007286	P:spermatid development	NAS:RGD.
GO:0006228	P:UTP biosynthetic process	IEA:InterPro.

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Interpro

IPR001564;    Nucleoside_diP_kinase.
IPR012336;    Thioredoxin-like_fold.
IPR017937;    Thioredoxin_CS.
IPR013766;    Thioredoxin_domain.
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Pfam

PF00334;    NDK;    3.
PF00085;    Thioredoxin;    1.
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SMART

SM00562;    NDK;    2.
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PROSITE

PS00469;    NDP_KINASES;    FALSE_NEG.
PS00194;    THIOREDOXIN_1;    1.
PS51352;    THIOREDOXIN_2;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: 1787 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 587 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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