SpermatogenesisOnline 1.0
 

Tag Content
SG ID
SG00002082 
UniProt Accession
Theoretical PI
5.63  
Molecular Weight
60578 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Pak1 
Gene Synonyms/Alias
 
Protein Name
Serine/threonine-protein kinase PAK 1 
Protein Synonyms/Alias
EC=2.7.11.1 Alpha-PAK; Protein kinase MUK2; p21-activated kinase 1;PAK-1 p68-PAK; 
Organism
Rattus norvegicus (Rat) 
NCBI Taxonomy ID
10116 
Chromosome Location
chr:1;155057622-155174708;1
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Description
Temporarily unavailable 
The information of related literatures
1. R. A. Wang, M. Zhao, M. L. Meistrich and R. Kumar (2005) Stage-specific expression of dynein light chain-1 and its interacting kinase, p21-activated kinase-1, in rodent testes. J Histochem Cytochem 53(10): 1235-43. 

Abstract
Mammalian spermatogenesis is a complex process involving regulatory interactions of many gene products. In this study, we found that dynein light chain-1 (DLC1), a component of the dynein motor complex, is highly expressed in mouse and rat testes. Immunohistochemically detectable levels of DLC1 are observed specifically in spermatids in steps 9-16 in distinct subcellular compartments PMID: [15983119] 

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Figures for illustrating the function of this protein/gene
Ref: R. A. Wang, M. Zhao, M. L. Meistrich and R. Kumar (2005) Stage-specific expression of dynein light chain-1 and its interacting kinase, p21-activated kinase-1, in rodent testes. J Histochem Cytochem 53(10): 1235-43. PMID: [15983119]
Ref: R. A. Wang, M. Zhao, M. L. Meistrich and R. Kumar (2005) Stage-specific expression of dynein light chain-1 and its interacting kinase, p21-activated kinase-1, in rodent testes. J Histochem Cytochem 53(10): 1235-43. PMID: [15983119]
Ref: R. A. Wang, M. Zhao, M. L. Meistrich and R. Kumar (2005) Stage-specific expression of dynein light chain-1 and its interacting kinase, p21-activated kinase-1, in rodent testes. J Histochem Cytochem 53(10): 1235-43. PMID: [15983119]
Ref: R. A. Wang, M. Zhao, M. L. Meistrich and R. Kumar (2005) Stage-specific expression of dynein light chain-1 and its interacting kinase, p21-activated kinase-1, in rodent testes. J Histochem Cytochem 53(10): 1235-43. PMID: [15983119]
Ref: R. A. Wang, M. Zhao, M. L. Meistrich and R. Kumar (2005) Stage-specific expression of dynein light chain-1 and its interacting kinase, p21-activated kinase-1, in rodent testes. J Histochem Cytochem 53(10): 1235-43. PMID: [15983119]
Function
The activated kinase acts on a variety of targets.Likely to be the GTPase effector that links the Rho-relatedGTPases to the JNK MAP kinase pathway. Activated by CDC42 andRAC1. Involved in dissolution of stress fibers and reorganizationof focal complexes. Involved in regulation of microtubulebiogenesis through phosphorylation of TBCB. DVL1 and PAK1 form aternary complex with MUSK which is important for MUSK-dependentregulation of AChR clustering during the formation of theneuromuscular junction (NMJ). Activity is inhibited in cellsundergoing apoptosis, potentially due to binding of CDC2L1 andCDC2L2. Phosphorylates MYL9/MLC2 (By similarity). PhosphorylatesRAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1,stimulation of RAF1 translocation to mitochondria, phosphorylationof BAD by RAF1, and RAF1 binding to BCL2 (By similarity). 
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Subcellular Location
 
Tissue Specificity
Expressed predominantly in the brain, withhigher expression in neuronal groups associated with motorfunction, and at lower levels in the spleen. 
Gene Ontology
GO IDGO termEvidence
GO:0030424 C:axon IDA:RGD.
GO:0005829 C:cytosol IDA:RGD.
GO:0030425 C:dendrite IDA:RGD.
GO:0005794 C:Golgi apparatus ISS:UniProtKB.
GO:0030426 C:growth cone IEA:Compara.
GO:0014704 C:intercalated disc IDA:RGD.
GO:0005624 C:membrane fraction IDA:RGD.
GO:0031965 C:nuclear membrane IDA:RGD.
GO:0005886 C:plasma membrane ISS:UniProtKB.
GO:0043234 C:protein complex IEA:Compara.
GO:0001726 C:ruffle ISS:UniProtKB.
GO:0030018 C:Z disc IDA:RGD.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0004709 F:MAP kinase kinase kinase activity TAS:RGD.
GO:0031532 P:actin cytoskeleton reorganization ISS:UniProtKB.
GO:0048754 P:branching morphogenesis of a tube ISS:UniProtKB.
GO:0032869 P:cellular response to insulin stimulus IDA:RGD.
GO:0033554 P:cellular response to stress IDA:RGD.
GO:0016358 P:dendrite development IEA:Compara.
GO:0060244 P:negative regulation of cell proliferation involved in contact inhibition ISS:UniProtKB.
GO:0007528 P:neuromuscular junction development IEA:Compara.
GO:0048812 P:neuron projection morphogenesis IMP:RGD.
GO:0033148 P:positive regulation of intracellular estrogen receptor signaling pathway ISS:UniProtKB.
GO:0043507 P:positive regulation of JUN kinase activity ISS:UniProtKB.
GO:0033138 P:positive regulation of peptidyl-serine phosphorylation ISS:UniProtKB.
GO:0051496 P:positive regulation of stress fiber assembly ISS:UniProtKB.
GO:0046777 P:protein autophosphorylation ISS:UniProtKB.
GO:0043113 P:receptor clustering IEA:Compara.
GO:0001666 P:response to hypoxia IDA:RGD.
GO:0007264 P:small GTPase mediated signal transduction TAS:RGD.
GO:0042060 P:wound healing ISS:UniProtKB.
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Interpro
IPR011009;    Kinase-like_dom.
IPR000095;    PAK_box_Rho-bd.
IPR000719;    Prot_kinase_cat_dom.
IPR017441;    Protein_kinase_ATP_BS.
IPR002290;    Ser/Thr_dual-sp_kinase_dom.
IPR008271;    Ser/Thr_kinase_AS.
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Pfam
PF00786;    PBD;    1.
PF00069;    Pkinase;    1.
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SMART
SM00285;    PBD;    1.
SM00220;    S_TKc;    1.
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PROSITE
PS50108;    CRIB;    1.
PS00107;    PROTEIN_KINASE_ATP;    1.
PS50011;    PROTEIN_KINASE_DOM;    1.
PS00108;    PROTEIN_KINASE_ST;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
Experiment identified 
Protein sequence Annotation
CHAIN         1    544       Serine/threonine-protein kinase PAK 1.
                             /FTId=PRO_0000086462.
DOMAIN       75     88       CRIB.
DOMAIN      269    520       Protein kinase.
NP_BIND     275    283       ATP (By similarity).
REGION       70    140       Autoregulatory region (By similarity).
REGION       70    105       GTPase-binding.
REGION      132    269       Interaction with CRIPAK (By similarity).
ACT_SITE    388    388       Proton acceptor (By similarity).
BINDING     298    298       ATP (By similarity).
MOD_RES      21     21       Phosphoserine; by autocatalysis
                             (Probable).
MOD_RES      57     57       Phosphoserine; by autocatalysis
                             (Probable).
MOD_RES      84     84       Phosphothreonine; by OXSR1.
MOD_RES     144    144       Phosphoserine; by autocatalysis
                             (Probable).
MOD_RES     149    149       Phosphoserine; by autocatalysis
                             (Probable).
MOD_RES     198    198       Phosphoserine; by autocatalysis
                             (Probable).
MOD_RES     203    203       Phosphoserine; by autocatalysis.
MOD_RES     211    211       Phosphothreonine (By similarity).
MOD_RES     218    218       Phosphothreonine (By similarity).
MOD_RES     219    219       Phosphoserine (By similarity).
MOD_RES     222    222       Phosphoserine (By similarity).
MOD_RES     224    224       Phosphothreonine (By similarity).
MOD_RES     228    228       Phosphothreonine (By similarity).
MOD_RES     229    229       Phosphothreonine (By similarity).
MOD_RES     255    255       N6-acetyllysine (By similarity).
MOD_RES     422    422       Phosphothreonine; by autocatalysis and
                             PDPK1.
MOD_RES     473    473       Phosphotyrosine (By similarity).
MUTAGEN      84     84       T->A: Constitutively active.
MUTAGEN      84     84       T->E: Inhibits activation by binding to
                             CDC42.
MUTAGEN     144    144       S->A: Decreases activity; when associated
                             with A-149.
MUTAGEN     149    149       S->A: Decreases activity; when associated
                             with A-144.
MUTAGEN     422    422       T->A: Decreases activity.
STRAND       88     90
HELIX        91     93
STRAND       95     98
HELIX       101    106
STRAND      107    109
STRAND      113    115
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Nucleotide Sequence
Length: 2223 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 544 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Arhgef7MINT 
_MINT 
Arhgef7MINT 
CDC42IntAct 
OXSR1IntAct 
_IntAct 
VimIntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Temporarily unavailable 
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