SG ID

SG00002107 

UniProt Accession

F1LWE7_RAT;F1LWE7;  

Theoretical PI

FRAGMENT  

Molecular Weight

28168 Da  

Genbank Nucleotide ID

 

Genbank Protein ID

 

Gene Name

 

Gene Synonyms/Alias

 

Protein Name

 

Protein Synonyms/Alias

SubName: Uncharacterized proteinFlags: Fragment 

Rattus norvegicus (Rat) 

10116 

Temporarily unavailable 

The information of related literatures

1. M. Salanova, S. Y. Chun, S. Iona, C. Puri, M. Stefanini and M. Conti (1999) Type 4 cyclic adenosine monophosphate-specific phosphodiesterases are expressed in discrete subcellular compartments during rat spermiogenesis. Endocrinology 140(5): 2297-306. 

Abstract
The type 4 cAMP-specific phosphodiesterases (PDE4) are a family of closely related enzymes with similar catalytic domains and divergent amino- and carboxyl-terminus domains. Multiple PDE proteins with heterogeneous amino termini are derived from each gene. To understand the significance of this heterogeneity, the expression and localization of variants derived from PDE4A and PDE4D genes was investigated during spermatogenesis in the rat. RNase protection analysis with mRNA for testes at different ages of development showed that two transcripts (PDE4D1 and PDE4D2) are expressed at day 10 and 15 of age and become undetectable thereafter. An additional PDE4D transcript appears at day 30 and increased during testid maturation. This latter transcript codes for a long variant of the PDE4D gene and is expressed in germ cells as demonstrated by RNase protection with RNA from isolated pachytene spermatocytes and round spermatids. The presence of a corresponding PDE4D protein with a molecular mass of 98 kDa was established by immunoprecipitation and Western blot analysis with antibodies specific for PDE4D and by immunoaffinity chromatography purification of the 98 kDa variant from isolated germ cells. PDE4A transcripts were also expressed in pachytene spermatocytes and round spermatids. Two polypeptides encoded by these PDE4A transcripts were expressed in pachytene spermatocytes, reached a maximum in round spermatids, and declined thereafter. Immunofluorescence analysis demonstrated a localization of the PDE4D protein in the manchette and in a periacrosomal region of the developing spermatid, a localization confirmed by immunogold electron microscopy. Conversely, the PDE4A was mostly soluble in the cytoplasm of round spermatids. These data demonstrate that PDE4D and PDE4A variants are expressed at different stages and localized in distinct subcellular structures of developing spermatids. Different properties of the mRNAs derived from the two genes and localization signals are responsible for the temporal and spatial expression of the different PDE4 isoenzymes. PMID: [10218983] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0004114	F:3',5'-cyclic-nucleotide phosphodiesterase activity	IEA:InterPro.
GO:0030552	F:cAMP binding	IEA:Compara.
GO:0008144	F:drug binding	IEA:Compara.
GO:0006198	P:cAMP catabolic process	IEA:Compara.
GO:0007165	P:signal transduction	IEA:InterPro.
GO:0006939	P:smooth muscle contraction	IEA:Compara.

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Interpro

IPR023088;    PDEase.
IPR002073;    PDEase_catalytic_dom.
IPR023174;    PDEase_CS.
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Pfam

PF00233;    PDEase_I;    1.
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SMART

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PROSITE

PS00126;    PDEASE_I;    1.
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PRINTS

PR00387;    PDIESTERASE1.;   
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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 247 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

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