SG ID

SG00002116 

UniProt Accession

G4VKS4_SCHMA;G4VKS4;  

Theoretical PI

8.58  

Molecular Weight

84149 Da  

Genbank Nucleotide ID

HE601629;  

Genbank Protein ID

CCD80719.1; 

Gene Name

GN ORFNames=Smp_149460 

Gene Synonyms/Alias

 

Protein Name

 

Protein Synonyms/Alias

SubName: Tyrosine kinase 

Schistosoma mansoni (Blood fluke) 

6183 

Temporarily unavailable 

The information of related literatures

1. S. Beckmann, C. Buro, C. Dissous, J. Hirzmann and C. G. Grevelding (2010) The Syk kinase SmTK4 of Schistosoma mansoni is involved in the regulation of spermatogenesis and oogenesis. PLoS Pathog 6(2): e1000769. 

Abstract
The signal transduction protein SmTK4 from Schistosoma mansoni belongs to the family of Syk kinases. In vertebrates, Syk kinases are known to play specialized roles in signaling pathways in cells of the hematopoietic system. Although Syk kinases were identified in some invertebrates, their role in this group of animals has not yet been elucidated. Since SmTK4 is the first Syk kinase from a parasitic helminth, shown to be predominantly expressed in the testes and ovary of adult worms, we investigated its function. To unravel signaling cascades in which SmTK4 is involved, yeast two-/three-hybrid library screenings were performed with either the tandem SH2-domain, or with the linker region including the tyrosine kinase domain of SmTK4. Besides the Src kinase SmTK3 we identified a new Src kinase (SmTK6) acting upstream of SmTK4 and a MAPK-activating protein, as well as mapmodulin acting downstream. Their identities and colocalization studies pointed to a role of SmTK4 in a signaling cascade regulating the proliferation and/or differentiation of cells in the gonads of schistosomes. To confirm this decisive role we performed biochemical and molecular approaches to knock down SmTK4 combined with a novel protocol for confocal laser scanning microscopy for morphological analyses. Using the Syk kinase-specific inhibitor Piceatannol or by RNAi treatment of adult schistosomes in vitro, corresponding phenotypes were detected in the testes and ovary. In the Xenopus oocyte system it was finally confirmed that Piceatannol suppressed the activity of the catalytic kinase domain of SmTK4. Our findings demonstrate a pivotal role of SmTK4 in gametogenesis, a new function for Syk kinases in eukaryotes. PMID: [20169182] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005524	F:ATP binding	IEA:UniProtKB-KW.
GO:0004713	F:protein tyrosine kinase activity	IEA:UniProtKB-KW.

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Interpro

IPR011009;    Kinase-like_dom.
IPR000719;    Prot_kinase_cat_dom.
IPR017441;    Protein_kinase_ATP_BS.
IPR001245;    Ser-Thr/Tyr_kinase_cat_dom.
IPR008266;    Tyr_kinase_AS.
IPR020635;    Tyr_kinase_cat_dom.
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Pfam

PF07714;    Pkinase_Tyr;    1.
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SMART

SM00219;    TyrKc;    1.
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PROSITE

PS00107;    PROTEIN_KINASE_ATP;    1.
PS50011;    PROTEIN_KINASE_DOM;    1.
PS00109;    PROTEIN_KINASE_TYR;    1.
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PRINTS

PR00109;    TYRKINASE.;   
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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 766 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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