SG ID

SG00002123 

UniProt Accession

F1SF43_PIG;F1SF43;  

Theoretical PI

8.88  

Molecular Weight

43077 Da  

Genbank Nucleotide ID

CU914424;  

Genbank Protein ID

 

Gene Name

GJA1 

Gene Synonyms/Alias

 

Protein Name

Gap junction protein 

Protein Synonyms/Alias

 

Sus scrofa (Pig) 

9823 

Temporarily unavailable 

The information of related literatures

1. I. Kopera, M. Durlej, A. Hejmej, K. Knapczyk-Stwora, M. Duda, M. Slomczynska and B. Bilinska (2011) Differential expression of connexin 43 in adult pig testes during normal spermatogenic cycle and after flutamide treatment. Reprod Domest Anim 46(6): 1050-60. 

Abstract
Evidence is mounting that the foetal and neonatal period of reproductive tract development is highly sensitive to hormonal disruption induced by various endocrine active compounds. Thus, we asked whether androgen withdrawal caused by prenatal (GD20, GD80) or neonatal (PD2) exposure to an anti-androgen flutamide alters Cx43 gene expression and may induce delayed effects on morphology and function of adult pig testes. Flutamide was given in five doses (50 mg/kg bw). Our histological analysis and TUNEL staining revealed varying degrees of seminiferous tubules abnormalities in all experimental pigs. Testes of pigs exposed to flutamide in utero exhibited moderate alterations of the spermatogenic process, whereas those of exposed neonatally were severely impaired. The most striking effects were spermatogenic arrest, germ cell detachment and a statistically significant increase in the frequency of germ cell apoptosis (p<0.01). Moreover, all pigs exposed to flutamide displayed Leydig cell hyperplasia. Because the network of cell-cell communication provided by gap junction channels plays an essential role in the regulation and maintenance of spermatogenesis, the physiological significance of Cx43-based gap junctions with regards to the gonadal impairment was evaluated by analysis of its expression using immunohistochemical, Western blot and qRT-PCR approaches. Significantly, lower Cx43 expression was found when flutamide was administered neonatally, which has coincided with severe disruption of spermatogenesis. Our data suggest that neonatal exposure to flutamide induces long-term effects on the spermatogenic capacity of the pig testis through alterations of Cx43-mediated intercellular communication and permanent alteration of both Sertoli and Leydig cell functions. PMID: [21457361] 

Figures for illustrating the function of this protein/gene

Function

One gap junction consists of a cluster of closely packedpairs of transmembrane channels, the connexons, through whichmaterials of low MW diffuse from one cell to a neighboring cell(By similarity). 

Subcellular Location

Membrane; Multi-pass membrane protein (Bysimilarity). 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005922	C:connexon complex	IEA:InterPro.
GO:0043292	C:contractile fiber	IEA:Compara.
GO:0005829	C:cytosol	IEA:Compara.
GO:0005916	C:fascia adherens	IEA:Compara.
GO:0016021	C:integral to membrane	IEA:UniProtKB-KW.
GO:0005243	F:gap junction channel activity	IEA:Compara.
GO:0004871	F:signal transducer activity	IEA:Compara.
GO:0007512	P:adult heart development	IEA:Compara.
GO:0006915	P:apoptotic process	IEA:Compara.
GO:0048514	P:blood vessel morphogenesis	IEA:Compara.
GO:0007267	P:cell-cell signaling	IEA:Compara.
GO:0002070	P:epithelial cell maturation	IEA:Compara.
GO:0001947	P:heart looping	IEA:Compara.
GO:0001701	P:in utero embryonic development	IEA:Compara.
GO:0060174	P:limb bud formation	IEA:Compara.
GO:0010629	P:negative regulation of gene expression	IEA:Compara.
GO:0001764	P:neuron migration	IEA:Compara.
GO:0010628	P:positive regulation of gene expression	IEA:Compara.
GO:0043123	P:positive regulation of I-kappaB kinase/NF-kappaB cascade	IEA:Compara.
GO:0045844	P:positive regulation of striated muscle tissue development	IEA:Compara.
GO:0008016	P:regulation of heart contraction	IEA:Compara.
GO:0043403	P:skeletal muscle tissue regeneration	IEA:Compara.
GO:0055085	P:transmembrane transport	IEA:GOC.

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Interpro

IPR000500;    Connexin.
IPR002261;    Connexin43.
IPR013124;    Connexin43_C.
IPR019570;    Connexin_CCC.
IPR017990;    Connexin_CS.
IPR013092;    Connexin_N.
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Pfam

PF00029;    Connexin;    1.
PF03508;    Connexin43;    1.
PF10582;    Connexin_CCC;    1.
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SMART

SM00037;    CNX;    1.
SM01089;    Connexin_CCC;    1.
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PROSITE

PS00407;    CONNEXINS_1;    1.
PS00408;    CONNEXINS_2;    1.
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PRINTS

PR00206;    CONNEXIN.;   
PR01132;    CONNEXINA1.;   
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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 382 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Temporarily unavailable 

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