SG ID

SG00002125 

UniProt Accession

F1SRR6_PIG;F1SRR6;  

Theoretical PI

8.8  

Molecular Weight

58886 Da  

Genbank Nucleotide ID

AEMK01180761; CU686778;  

Genbank Protein ID

 

Gene Name

ER2 

Gene Synonyms/Alias

 

Protein Name

 

Protein Synonyms/Alias

SubName: Uncharacterized protein 

Sus scrofa (Pig) 

9823 

Temporarily unavailable 

The information of related literatures

1. O. Lekhkota, R. Brehm, R. Claus, A. Wagner, R. M. Bohle and M. Bergmann (2006) Cellular localization of estrogen receptor-alpha (ERalpha) and -beta (ERbeta) mRNA in the boar testis. Histochem Cell Biol 125(3): 259-64. 

Abstract
Boar testes synthesize high amounts of estrogens which are known to stimulate several male sexual functions in a variety of extragonadal target tissues. Possible effects within the testis depend on the existence of the estrogen receptor subtypes alpha and beta (ERalpha, ERbeta). The precise cellular localization of these subtypes within the testis was, so far, based mainly on protein expression studies using different antibodies in several species including boars shows contradictory results. Therefore, we investigated the ERalpha and ERbeta gene expression using RT-PCR of testis homogenates and RT-PCR after UV-single cell microdissection combined with in-situ hybridization of four fertile boars with an average age of 32 weeks. Both ERalpha and ERbeta mRNA were found in testis homogenates. Using in-situ hybridization and UV-single cell microdissection ERalpha mRNA was present in type A and type B spermatogonia up to mid-pachytene primary spermatocytes in stage V-VIII and stage I of the seminiferous epithelial cycle, but not in other cells. ERbeta mRNA was found only in Sertoli cells. Interstitial Leydig cells revealed neither ERalpha nor ERbeta mRNA. The data suggest a direct impact of estrogen in the boar on Sertoli cell function via ERbeta and germ cell formation via ERalpha. PMID: [16249893] 

2. H. M. Mutembei, S. Pesch, G. Schuler and B. Hoffmann (2005) Expression of oestrogen receptors alpha and beta and of aromatase in the testis of immature and mature boars. Reprod Domest Anim 40(3): 228-36. 

Abstract
The boar testis secretes high amounts of oestrogens. In order to test for a likely local significance, we investigated the expression of oestrogen receptors (ER) in immature and mature boar testes using immunohistochemistry (IHC), in vitro and in situ reverse transcription-polymerase chain reaction (RT-PCR). Samples were from 25 boars castrated at ages of 50, 100, 150, 200 and 250 days. Mouse monoclonal primary antibodies against porcine ERalpha (clone HT227), human ERbeta1 (clone PPG5/10) and human P450 aromatase (clone SM1671P) were used. Expression of the mRNA was tested utilizing primers specific for the respective porcine mRNA sequences. ER immunoreactivity was exclusively localized to the nuclei. In immature boars, 90.6 +/- 1.2% of prespermatogonia and 71.0 +/- 2.6% of the Leydig cells showed a strong staining for ERalpha; 95.5 +/- 3.5% of the prespermatogonia but none of the Leydig and Sertoli cells were ERbeta-positive. In mature boars a strong staining for the ERbeta was observed in virtually all Sertoli, Leydig and germ cells, except for the elongating/ed spermatids, which were clearly negative; for the ERalpha, strong immunoreaction signals were restricted to spermatogonia and primary spermatocytes with 93.6 +/- 2.7% of these cells being positive; distinctly less intensive signals were observed in 51.4 +/- 0.27% of the secondary spermatocytes, round spermatids and Leydig cells. In vitro RT-PCR was positive for both receptors and results of in situ RT-PCR matched those obtained by IHC. P450 aromatase immunoreaction was restricted to the cytoplasm of Leydig cells. These findings suggest that testicular ER may be important factors contributing to onset and maintenance of spermatogenesis in the boar. PMID: [15943697] 

Figures for illustrating the function of this protein/gene

Function

 

Subcellular Location

 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005739	C:mitochondrion	IEA:Compara.
GO:0005634	C:nucleus	IEA:UniProtKB-KW.
GO:0001046	F:core promoter sequence-specific DNA binding	IEA:Compara.
GO:0034056	F:estrogen response element binding	IEA:Compara.
GO:0003700	F:sequence-specific DNA binding transcription factor activity	IEA:InterPro.
GO:0005496	F:steroid binding	IEA:InterPro.
GO:0003707	F:steroid hormone receptor activity	IEA:InterPro.
GO:0008270	F:zinc ion binding	IEA:InterPro.
GO:0007420	P:brain development	IEA:Compara.
GO:0060743	P:epithelial cell maturation involved in prostate gland development	IEA:Compara.
GO:0030520	P:intracellular estrogen receptor signaling pathway	IEA:Compara.
GO:0060766	P:negative regulation of androgen receptor signaling pathway	IEA:Compara.
GO:0050680	P:negative regulation of epithelial cell proliferation	IEA:Compara.
GO:0000122	P:negative regulation of transcription from RNA polymerase II promoter	IEA:Compara.
GO:0001764	P:neuron migration	IEA:Compara.
GO:0001541	P:ovarian follicle development	IEA:Compara.
GO:0043065	P:positive regulation of apoptotic process	IEA:Compara.
GO:0051091	P:positive regulation of sequence-specific DNA binding transcription factor activity	IEA:Compara.
GO:0060740	P:prostate gland epithelium morphogenesis	IEA:Compara.
GO:0006351	P:transcription, DNA-dependent	IEA:UniProtKB-KW.
GO:0060065	P:uterus development	IEA:Compara.
GO:0060068	P:vagina development	IEA:Compara.

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Interpro

IPR021064;    Estrogen_rcpt_beta_N.
IPR008946;    Nucl_hormone_rcpt_ligand-bd.
IPR000536;    Nucl_hrmn_rcpt_lig-bd_core.
IPR024178;    Oestrogen_rcpt-rel.
IPR001723;    Str_hrmn_rcpt.
IPR001628;    Znf_hrmn_rcpt.
IPR013088;    Znf_NHR/GATA.
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Pfam

PF12497;    ERbeta_N;    1.
PF00104;    Hormone_recep;    1.
PF00105;    zf-C4;    1.
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SMART

SM00430;    HOLI;    1.
SM00399;    ZnF_C4;    1.
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PROSITE

PS00031;    NUCLEAR_REC_DBD_1;    1.
PS51030;    NUCLEAR_REC_DBD_2;    1.
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PRINTS

PR00398;    STRDHORMONER.;   
PR00047;    STROIDFINGER.;   
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Created Date

18-Oct-2012 

Record Type

Experiment identified 

Protein sequence Annotation

Nucleotide Sequence

Length: bp   Go to nucleotide: FASTA

Protein Sequence

Length: 526 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

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Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

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View Microarray data

Temporarily unavailable 

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