SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00002151 
UniProt Accession
Theoretical PI
8.62  
Molecular Weight
38619 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Apoh 
Gene Synonyms/Alias
B2gp1 
Protein Name
Beta-2-glycoprotein 1 
Protein Synonyms/Alias
APC inhibitor; Activated protein C-binding protein; Apolipoprotein H;Apo-H Beta-2-glycoprotein I;B2GPIBeta(2)GPIFlags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:11;108204668-108275710;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.129237386 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. beta 2-Glycoprotein I (beta 2 GPI), a plasma protein that binds to anionic phospholipids, is composed of five repeating units called a short consensus repeat (SCR), which is found mostly in the regulatory proteins of the complement system. Recently the human beta 2 GPI gene has been assigned to chromosome 17, not to chromosome 1 where most of the genes of the SCR-containing proteins are clustered. In this report, we have isolated a full-length cDNA clone of mouse beta 2 GPI and determined the chromosomal localization of the gene. The amino acid sequence deduced from the nucleotide sequence of mouse beta 2 GPI revealed 76.1% identity with that of human beta 2 GPI. A genetic mapping by in situ hybridization and linkage analysis using 50 backcross mice has shown that the mouse beta 2 GPI gene (designated B2gp1) is located on the terminal portion of the D region of chromosome 11, closely linked to Gfap, and is 18 cM distal to Acrb, extending a conserved linkage group between mouse chromosome 11 and human chromosome 17. On the basis of these results, the evolutionary relationships among the SCR-containing proteins are discussed. PMID: [1339387] 

2. beta 2-glycoprotein I (beta 2I) is a 50kDa serum glycoprotein of ill defined function. Based upon its capacity to bind negatively charged phospholipids a number of possible inhibitory roles for beta 2I have been proposed. We have cloned and sequenced a full length mouse beta 2I cDNA clone and demonstrated that mouse beta 2I does not behave as an acute phase reactant following an experimentally induced inflammation. Phylogenetic analysis of the known mammalian beta 2I homologues has provided evidence that mouse beta 2I is the most divergent and is evolving at a faster rate than beta 2I in other species. PMID: [7514402] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

4. A procedure to map N-glycosylation sites is presented here. It can be applied to purified proteins as well as to highly complex mixtures. The method exploits deglycosylation by PNGase F in a diagonal, reverse-phase chromatographic setup. When applied to 10 microL of mouse serum, affinity-depleted for its three most abundant components, 117 known or predicted sites were mapped in addition to 10 novel sites. Several sites were detected on soluble membrane or receptor components. Our method furthermore senses the nature of glycan structures and can detect differential glycosylation on a given site. These properties--high sensitivity and dependence on glycan imprinting--can be exploited for glycan-biomarker analysis. PMID: [16944957] 

5. A comprehensive understanding of the mouse plasma proteome is important for studies using mouse models to identify protein markers of human disease. To enhance our analysis of the mouse plasma proteome, we have developed a method for isolating low-abundance proteins using a cysteine-containing glycopeptide strategy. This method involves two orthogonal affinity capture steps. First, glycoproteins are coupled to an azlactone copolymer gel using hydrazide chemistry and cysteine residues are then biotinylated. After trypsinization and extensive washing, tethered N-glycosylated tryptic peptides are released from the gel using PNGase F. Biotinylated cysteinyl-containing glycopeptides are then affinity selected using a monomeric avidin gel and analyzed by LC-MS/MS. We have applied the method to a proteome analysis of mouse plasma. In two independent analyses using 200 muL each of C57BL mouse plasma, 51 proteins were detected. Only 42 proteins were seen when the same plasma sample was analyzed by glycopeptides only. A total of 104 N-glycosylation sites were identified. Of these, 17 sites have hitherto not been annotated in the Swiss-Prot database whereas 48 were considered probable, potential, or by similarity - i.e., based on little or no experimental evidence. We show that analysis by cysteine-containing glycopeptides allows detection of low-abundance proteins such as the epidermal growth factor receptor, the Vitamin K-dependent protein Z, the hepatocyte growth factor activator, and the lymphatic endothelium-specific hyaluronan receptor as these proteins were not detected in the glycopeptide control analysis. PMID: [17330941] 

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Function
Binds to various kinds of negatively charged substancessuch as heparin, phospholipids, and dextran sulfate. May preventactivation of the intrinsic blood coagulation cascade by bindingto phospholipids on the surface of damaged cells. 
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Subcellular Location
Secreted. 
Tissue Specificity
Expressed by the liver and secreted in plasma. 
Gene Ontology
GO IDGO termEvidence
GO:0009986 C:cell surface IEA:Compara.
GO:0042627 C:chylomicron IEA:Compara.
GO:0031012 C:extracellular matrix IEA:Compara.
GO:0005615 C:extracellular space IDA:MGI.
GO:0034364 C:high-density lipoprotein particle IEA:Compara.
GO:0034361 C:very-low-density lipoprotein particle IEA:Compara.
GO:0043499 F:eukaryotic cell surface binding IEA:Compara.
GO:0008201 F:heparin binding IEA:UniProtKB-KW.
GO:0060230 F:lipoprotein lipase activator activity IEA:Compara.
GO:0005543 F:phospholipid binding IEA:Compara.
GO:0007597 P:blood coagulation, intrinsic pathway IEA:Compara.
GO:0016525 P:negative regulation of angiogenesis IEA:Compara.
GO:0030195 P:negative regulation of blood coagulation IEA:Compara.
GO:0010596 P:negative regulation of endothelial cell migration IEA:Compara.
GO:0001937 P:negative regulation of endothelial cell proliferation IEA:Compara.
GO:0051918 P:negative regulation of fibrinolysis IEA:Compara.
GO:0033033 P:negative regulation of myeloid cell apoptotic process IEA:Compara.
GO:0034392 P:negative regulation of smooth muscle cell apoptotic process IEA:Compara.
GO:0031100 P:organ regeneration IEA:Compara.
GO:0031639 P:plasminogen activation IEA:Compara.
GO:0051006 P:positive regulation of lipoprotein lipase activity IEA:Compara.
GO:0030193 P:regulation of blood coagulation IMP:MGI.
GO:0006641 P:triglyceride metabolic process IEA:Compara.
GO:0034197 P:triglyceride transport IEA:Compara.
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Interpro
IPR016060;    Complement_control_module.
IPR015104;    Sushi_2.
IPR000436;    Sushi_SCR_CCP.
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Pfam
PF00084;    Sushi;    4.
PF09014;    Sushi_2;    1.
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SMART
SM00032;    CCP;    5.
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PROSITE
PS50923;    SUSHI;    4.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
SIGNAL        1     19
CHAIN        20    345       Beta-2-glycoprotein 1.
                             /FTId=PRO_0000002060.
DOMAIN       21     81       Sushi 1.
DOMAIN       82    139       Sushi 2.
DOMAIN      140    202       Sushi 3.
DOMAIN      203    262       Sushi 4.
REGION      263    345       Sushi-like.
CARBOHYD    105    105       N-linked (GlcNAc...).
CARBOHYD    117    117       N-linked (GlcNAc...).
CARBOHYD    162    162       N-linked (GlcNAc...).
CARBOHYD    183    183       N-linked (GlcNAc...).
CARBOHYD    193    193       N-linked (GlcNAc...).
DISULFID     23     66       By similarity.
DISULFID     51     79       By similarity.
DISULFID     84    124       By similarity.
DISULFID    110    137       By similarity.
DISULFID    142    188       By similarity.
DISULFID    174    200       By similarity.
DISULFID    205    248       By similarity.
DISULFID    234    260       By similarity.
DISULFID    264    315       By similarity.
DISULFID    300    325       By similarity.
DISULFID    307    345       By similarity.
CONFLICT    252    252       G -> R (in Ref. 2; AAB30789).
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Nucleotide Sequence
Length: 1158 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 345 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Kcnma1IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
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