Tag Content
UniProt Accession
Theoretical PI
Molecular Weight
17296 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gene Synonyms/Alias
Protein Name
Sperm surface protein Sp17 
Protein Synonyms/Alias
Sperm autoantigenic protein 17; 
Mus musculus (Mouse) 
NCBI Taxonomy ID
Chromosome Location
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Probability (GAS) of Function in Spermatogenesis
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Temporarily unavailable 
Abstract of related literatures
1. The present study characterizes the sperm protein Sp17 in the mouse. Sp17 is a mammalian testis- and sperm-specific protein that has been isolated, sequenced, and characterized from rabbit testis and spermatozoa. In this study, a rabbit Sp17 cDNA probe representing the entire protein coding region was used to screen a mouse testis cDNA library to obtain the mouse Sp17 sequence. The mouse mRNa for Sp17 encodes a 149-amino acid protein with a predicted molecular weight of 17,296. The mouse Sp17 (MSp17) cDNA sequence is 82% identical to the rabbit Sp17 cDNA sequence while the MSp17 protein sequence is 74% identical to the rabbit protein sequence. The presence of native Sp17 in mouse spermatozoa and testis was demonstrated by Western blot analysis, immunoprecipitation, and immunolocalization. After SDS-PAGE, the native Sp17 has an apparent molecular mass of 24 kDa. The sequence of the native Sp17 was confirmed by Western blots of mouse testis and spermatozoa probed with two anti-peptide antibodies--one, anti-G22C, made against amino acids 61-82 in the rabbit sequence (61-83 in the mouse), and a second, anti-K18C, made against amino acids 120-136 in the C-terminal region in the human sequence (118-134 in the mouse sequence). In the absence of proteolytic inhibitors, part of the C-terminal of native MSp17 is cleaved, giving rise to an 18-kDa band. Sp17 is present in spermatocytes and spermatids in the testis. In spermatozoa, Sp17 is not available to bind antibody on the surface of live, acrosome-intact spermatozoa, but it is present on the equatorial surface of live, acrosome-reacted spermatozoa. In fixed spermatozoa, staining is observed along the length of the principal piece, weakly along the midpiece, and over the acrosomal region of the head. When the acrosome reaction begins, acrosomal staining is seen throughout the equatorial region of the acrosome. Using mimotope analysis, this study has also demonstrated that native Sp17 is a sperm autoantigen and that recombinant mouse Sp17 is immunogenic in males with a highly restricted linear epitope. PMID: [7578682] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Sperm surface zona pellucida binding protein. Helps tobind spermatozoa to the zona pellucida with high affinity. Mightfunction in binding zona pellucida and carbohydrates (Bysimilarity). 
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Subcellular Location
Membrane; Peripheral membrane protein(Potential). 
Tissue Specificity
Testis- and sperm-specific. 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IEA:Compara.
GO:0019861 C:flagellum IEA:Compara.
GO:0016020 C:membrane IEA:UniProtKB-SubCell.
GO:0031514 C:motile cilium IEA:Compara.
GO:0072372 C:primary cilium IEA:Compara.
GO:0008603 F:cAMP-dependent protein kinase regulator activity IEA:InterPro.
GO:0007339 P:binding of sperm to zona pellucida TAS:MGI.
GO:0001539 P:ciliary or flagellar motility IEA:Compara.
GO:0007165 P:signal transduction IEA:InterPro.
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IPR003117;    cAMP_dep_PK_reg_su_I/II_a/b.
IPR000048;    IQ_motif_EF-hand-BS.
IPR012105;    Sp17.
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PF00612;    IQ;    1.
PF02197;    RIIa;    1.
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SM00015;    IQ;    1.
SM00394;    RIIa;    1.
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PS50096;    IQ;    1.
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Created Date
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    149       Sperm surface protein Sp17.
DOMAIN      112    141       IQ.
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Nucleotide Sequence
Length: 599 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 149 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data