Probability (GAS) of Function in Spermatogenesis |
0.129188369
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis. |
Abstract of related literatures |
1. The human apolipoprotein E (APOE), APOC1, pseudo APOC1 (APOC1'), and APOC2 genes are clustered within 48 kb on the long arm of chromosome 19. A mouse Apoe cDNA probe was used to isolate overlapping cosmid clones from a cosmid library of the C57BL/Rij inbred mouse strain. These clones were investigated for the presence of the Apoc1 and Apoc2 genes by heterologous hybridization. Our results show that the Apoe-c1-c2 gene cluster is conserved in the mouse. In line with evolutionary data, the mouse lacks the equivalent of APOC1'. These data were confirmed using a mouse Apoc2 cDNA clone, and surprisingly the cDNA clone isolated here was 965 bp in size, which is on average 450 bp longer than other APOC2 cDNAs described so far. Correspondingly, the Apoc2 gene occupies an unusually large genomic region, due to an extended 5' end. Interestingly, a variable number of tandem repeat (VNTR) in the third intron of the human APOC2 gene shows a high sequence homology and is located at the identical position in the mouse gene. Despite the high copy number of this VNTR (27 or 34 copies) only two variants were found among 11 different inbred strains. With the aid of six restriction fragment length variations in this gene cluster only two different haplotypes could be deduced, indicating that the Apoe-c1-c2 gene cluster is highly conserved in the inbred strains that were studied. PMID: [7916738]
2. Three cDNA clones containing the mouse apolipoprotein C2 (Apoc2) gene were isolated from a mouse liver cDNA library. The inserts from two cDNA clones were 500 bp in size while the insert from the third clone was unexpectedly large, 962 bp. All three clones contained a single open reading frame encoding apoC2. The exon-intron structure of the mouse Apoc2 gene was determined by sequence analysis. Northern blotting and primer extension analysis of mouse RNA showed that the major liver transcript is 500 bp in size and is encoded by four exons. Transcripts for Apoc2 were found in fetal liver, adult liver, intestine, and peritoneal macrophages. The largest cDNA clone, mAPOC2c4, contained an additional 440 bp at the 5' end that are evolutionary conserved between man and mouse. These additional sequences are encoded by two exons located 5' to the major liver start site. Although the larger transcript could not be detected by Northern blot analysis, products resulting from an upstream transcription initiation site were detected in the liver using RT-PCR analysis. The sizes of the RT-PCR products are consistent with alternative splicing. PMID: [7691714]
3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334]
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Function |
Component of the very low density lipoprotein (VLDL)fraction in plasma, and is an activator of several triacylglycerollipases. The association of APOC2 with plasma chylomicrons, VLDL,and HDL is reversible, a function of the secretion and catabolismof triglyceride-rich lipoproteins, and changes rapidly. Back to Top |