SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00002688 
UniProt Accession
Theoretical PI
4.95  
Molecular Weight
53479 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Slc2a3 
Gene Synonyms/Alias
Glut-3, Glut3 
Protein Name
Solute carrier family 2, facilitated glucose transporter member 3 
Protein Synonyms/Alias
Glucose transporter type 3, brain;GLUT-3 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:6;122677827-122751658;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.905950648 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. Complementary DNAs encoding the mouse GLUT3/brain facilitative glucose transporter have been isolated and sequenced. The predicted amino acid sequence indicates that mouse GLUT3 is composed of 493 amino acids and has 83 and 89% identity and similarity, respectively, to the sequence of human GLUT3. In contrast to human GLUT3 mRNA, which can be readily detected by RNA blotting in all human tissues that have been examined, mouse GLUT3 mRNA was only present at significant levels in brain. In situ hybridization showed differential expression of GLUT3 mRNA in several regions of adult mouse brain. Specific expression was observed in the hippocampus, with GLUT3 mRNA levels being higher in areas CA1 to CA3 than in the dentate gyrus. It was also detected in the Purkinje cell layer of the cerebellum and in the cerebral cortex, with higher expression in the piriform cortex than in other regions of the cortex. Antisera to mouse GLUT3 immunoblotted a series of proteins of 45-50 kDa in mouse brain plasma membranes. These results are consistent with GLUT3 being a neuronal glucose transporter. PMID: [1730609] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

3. Although the classification of cell types often relies on the identification of cell surface proteins as differentiation markers, flow cytometry requires suitable antibodies and currently permits detection of only up to a dozen differentiation markers in a single measurement. We use multiplexed mass-spectrometric identification of several hundred N-linked glycosylation sites specifically from cell surface-exposed glycoproteins to phenotype cells without antibodies in an unbiased fashion and without a priori knowledge. We apply our cell surface-capturing (CSC) technology, which covalently labels extracellular glycan moieties on live cells, to the detection and relative quantitative comparison of the cell surface N-glycoproteomes of T and B cells, as well as to monitor changes in the abundance of cell surface N-glycoprotein markers during T-cell activation and the controlled differentiation of embryonic stem cells into the neural lineage. A snapshot view of the cell surface N-glycoproteins will enable detection of panels of N-glycoproteins as potential differentiation markers that are currently not accessible by other means. PMID: [19349973] 

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Function
Facilitative glucose transporter. Probably a neuronalglucose transporter. 
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Subcellular Location
Membrane; Multi-pass membrane protein. 
Tissue Specificity
Highly expressed in brain. 
Gene Ontology
GO IDGO termEvidence
GO:0002080 C:acrosomal membrane IDA:MGI.
GO:0005901 C:caveola IEA:Compara.
GO:0016021 C:integral to membrane IEA:UniProtKB-KW.
GO:0005886 C:plasma membrane IDA:UniProtKB.
GO:0030672 C:synaptic vesicle membrane IEA:Compara.
GO:0055056 F:D-glucose transmembrane transporter activity IDA:UniProtKB.
GO:0033300 F:dehydroascorbic acid transporter activity IDA:UniProtKB.
GO:0016936 F:galactoside binding IEA:Compara.
GO:0005536 F:glucose binding IEA:Compara.
GO:0033222 F:xylose binding IEA:Compara.
GO:0046323 P:glucose import IEA:Compara.
GO:0043066 P:negative regulation of apoptotic process IEA:Compara.
GO:0009749 P:response to glucose stimulus IEA:Compara.
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Interpro
IPR002945;    Glc_transpt_3.
IPR020846;    MFS_dom.
IPR016196;    MFS_dom_general_subst_transpt.
IPR005828;    Sub_transporter.
IPR003663;    Sugar/inositol_transpt.
IPR005829;    Sugar_transporter_CS.
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Pfam
PF00083;    Sugar_tr;    1.
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SMART
PROSITE
PS50850;    MFS;    1.
PS00216;    SUGAR_TRANSPORT_1;    1.
PS00217;    SUGAR_TRANSPORT_2;    1.
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PRINTS
PR01192;    GLUCTRSPORT3.;   
PR00171;    SUGRTRNSPORT.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    493       Solute carrier family 2, facilitated
                             glucose transporter member 3.
                             /FTId=PRO_0000050354.
TOPO_DOM      1     10       Cytoplasmic (Potential).
TRANSMEM     11     31       Helical; Name=1; (Potential).
TOPO_DOM     32     64       Extracellular (Potential).
TRANSMEM     65     85       Helical; Name=2; (Potential).
TOPO_DOM     86     93       Cytoplasmic (Potential).
TRANSMEM     94    114       Helical; Name=3; (Potential).
TOPO_DOM    115    124       Extracellular (Potential).
TRANSMEM    125    145       Helical; Name=4; (Potential).
TOPO_DOM    146    153       Cytoplasmic (Potential).
TRANSMEM    154    174       Helical; Name=5; (Potential).
TOPO_DOM    175    183       Extracellular (Potential).
TRANSMEM    184    204       Helical; Name=6; (Potential).
TOPO_DOM    205    269       Cytoplasmic (Potential).
TRANSMEM    270    290       Helical; Name=7; (Potential).
TOPO_DOM    291    304       Extracellular (Potential).
TRANSMEM    305    325       Helical; Name=8; (Potential).
TOPO_DOM    326    334       Cytoplasmic (Potential).
TRANSMEM    335    355       Helical; Name=9; (Potential).
TOPO_DOM    356    363       Extracellular (Potential).
TRANSMEM    364    384       Helical; Name=10; (Potential).
TOPO_DOM    385    399       Cytoplasmic (Potential).
TRANSMEM    400    420       Helical; Name=11; (Potential).
TOPO_DOM    421    427       Extracellular (Potential).
TRANSMEM    428    448       Helical; Name=12; (Potential).
TOPO_DOM    449    493       Cytoplasmic (Potential).
REGION      277    279       Defines substrate specificity (By
                             similarity).
MOD_RES     482    482       Phosphoserine (By similarity).
CARBOHYD     43     43       N-linked (GlcNAc...).
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Nucleotide Sequence
Length: 1774 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 493 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
H3f3aIntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
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