Tag Content
UniProt Accession
Theoretical PI
Molecular Weight
26635 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gene Synonyms/Alias
Fsc2, Gstm3 
Protein Name
Glutathione S-transferase Mu 5 
Protein Synonyms/Alias
EC= Fibrous sheath component 2;Fsc2 GST class-mu 5; 
Mus musculus (Mouse) 
NCBI Taxonomy ID
Chromosome Location
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Probability (GAS) of Function in Spermatogenesis
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Temporarily unavailable 
Abstract of related literatures
1. The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polymerase chain reaction (PCR) primers predicted from these two peptide sequences, a approximately 1.1 kb cDNA clone for fibrous sheath component 2 (Fsc2) was isolated which had 84% nucleic acid and 89% amino acid sequence identity with a previously reported mu-class human GST gene (hGSTM3; Campbell et al., 1990: J Biol Chem 265:4188-9193). Sequences corresponding to those of the two fibrous sheath peptides were present in the protein encoded by the Fsc2 cDNA. Northern analysis with the full length Fsc2 cDNA detected a approximately 1.1 kb mRNA in 12 of 15 somatic tissues examined, as well as in testis and isolated spermatogenic cells. However, 5'(nt--96 to 12) or 3'(nt 637 to 808) Fsc2 probes, containing mostly noncoding sequences, detected a approximately 1.1 kb mRNA abundant in testis and isolated spermatogenic cells, but absent or present at low levels in somatic tissues. Northern analysis with RNA from testes of mice of different postnatal ages and purified spermatogenic cell populations indicated that this transcript is first present during the meiotic phase of germ cell development. These results suggest that a previously unreported mu-class GST gene (mGSTM5.) is expressed at a specific time during the development of spermatogenic cells in the mouse. Immunoblot analysis indicated that a mu-class GST protein is associated with the fibrous sheath, suggesting that it becomes an integral part of the mouse sperm cytoskeleton. PMID: [8607970] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Conjugation of reduced glutathione to a wide number ofexogenous and endogenous hydrophobic electrophiles. 
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Subcellular Location
Tissue Specificity
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IEA:UniProtKB-SubCell.
GO:0005625 C:soluble fraction IEA:Compara.
GO:0004364 F:glutathione transferase activity IDA:MGI.
GO:0006749 P:glutathione metabolic process IEA:Compara.
GO:0043627 P:response to estrogen stimulus IEA:Compara.
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IPR010987;    Glutathione-S-Trfase_C-like.
IPR004045;    Glutathione_S-Trfase_N.
IPR017933;    Glutathione_S_Trfase/Cl_chnl_C.
IPR004046;    GST_C.
IPR003081;    GST_mu.
IPR012336;    Thioredoxin-like_fold.
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PF00043;    GST_C;    1.
PF02798;    GST_N;    1.
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PS50405;    GST_CTER;    1.
PS50404;    GST_NTER;    1.
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PR01267;    GSTRNSFRASEM.;   
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Created Date
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    224       Glutathione S-transferase Mu 5.
DOMAIN        4     91       GST N-terminal.
DOMAIN       93    211       GST C-terminal.
REGION       10     11       Glutathione binding (By similarity).
REGION       49     53       Glutathione binding (By similarity).
REGION       62     63       Glutathione binding (By similarity).
REGION       75     76       Glutathione binding (By similarity).
BINDING     119    119       Substrate (By similarity).
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Nucleotide Sequence
Length: 907 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 224 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data