SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00004559 
UniProt Accession
Theoretical PI
9.4  
Molecular Weight
52604 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Slc16a7 
Gene Synonyms/Alias
Mct2 
Protein Name
Monocarboxylate transporter 2 
Protein Synonyms/Alias
MCT 2 Solute carrier family 16 member 7; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:10;124663520-124766019;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.629809463 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. Although glucose is the major metabolic fuel needed for normal brain function, monocarboxylic acids, i.e., lactate, pyruvate, and ketone bodies, can also be utilized by the brain as alternative energy substrates. In most mammalian cells, these substrates are transported either into or out of the cell by a family of monocarboxylate transporters (MCTs), first cloned and sequenced in the hamster. We have recently cloned two MCT isoforms (MCT1 and MCT2) from a mouse kidney cDNA library. Northern blot analysis revealed that MCT1 mRNA is ubiquitous and can be detected in most tissues at a relatively constant level. MCT2 expression is more limited, with high levels of expression confined to testes, kidney, stomach, and liver and lower levels in lung, brain, and epididymal fat. Both MCT1 mRNA and MCT2 mRNA are detected in mouse brain using antisense riboprobes and in situ hybridization. MCT1 mRNA is found throughout the cortex, with higher levels of hybridization in hippocampus and cerebellum. MCT2 mRNA was detected in the same areas, but the pattern of expression was more specific. In addition, MCT1 mRNA, but not MCT2, is localized to the choroid plexus, ependyma, microvessels, and white matter structures such as the corpus callosum. These results suggest a differential expression of the two MCTs at the cellular level. PMID: [9725820] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. Delphilin, which interacts with a glutamate receptor (GluR) delta2-subunit, is a postsynaptic density scaffolding protein at the cerebellar parallel fiber-Purkinje cell synapses. Delphilin specifically interacts with the GluRdelta2 C-terminus via its postsynaptic density-95/discs-large/ZO-1 (PDZ) domain. As a number of PDZ-containing scaffolding proteins bind to several membrane proteins, we expected that Delphilin might also have other binding partners besides GluRdelta2. To search for the link between Delphilin and other binding proteins, we carried out screening among candidate membrane proteins localized in Purkinje cells by surface plasmon resonance analyses. As a result, we found that the C-terminus of the monocarboxylate transporter 2 binds specifically and significantly with Delphilin PDZ and there is a probable existence of GluRdelta2-Delphilin-monocarboxylate transporter 2 complex in synaptic membranes. PMID: [17496809] 

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Function
Proton-linked monocarboxylate transporter. Catalyzes therapid transport across the plasma membrane of manymonocarboxylates such as lactate, pyruvate, branched-chain oxoacids derived from leucine, valine and isoleucine, and the ketonebodies acetoacetate, beta-hydroxybutyrate and acetate. MCT2 is ahigh affinity pyruvate transporter. 
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Subcellular Location
Cell membrane; Multi-pass membrane protein(By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0016021 C:integral to membrane IEA:UniProtKB-KW.
GO:0005624 C:membrane fraction IEA:Compara.
GO:0005886 C:plasma membrane IEA:UniProtKB-SubCell.
GO:0015355 F:secondary active monocarboxylate transmembrane transporter activity IEA:InterPro.
GO:0015293 F:symporter activity IEA:UniProtKB-KW.
GO:0015711 P:organic anion transport IEA:InterPro.
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Interpro
IPR011701;    MFS.
IPR020846;    MFS_dom.
IPR016196;    MFS_dom_general_subst_transpt.
IPR004743;    Monocarb_transpt.
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Pfam
PF07690;    MFS_1;    1.
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SMART
PROSITE
PS50850;    MFS;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    484       Monocarboxylate transporter 2.
                             /FTId=PRO_0000211388.
TOPO_DOM      1     16       Cytoplasmic (Potential).
TRANSMEM     17     37       Helical; (Potential).
TOPO_DOM     38     60       Extracellular (Potential).
TRANSMEM     61     81       Helical; (Potential).
TOPO_DOM     82     90       Cytoplasmic (Potential).
TRANSMEM     91    111       Helical; (Potential).
TOPO_DOM    112    116       Extracellular (Potential).
TRANSMEM    117    137       Helical; (Potential).
TOPO_DOM    138    149       Cytoplasmic (Potential).
TRANSMEM    150    170       Helical; (Potential).
TOPO_DOM    171    174       Extracellular (Potential).
TRANSMEM    175    195       Helical; (Potential).
TOPO_DOM    196    245       Cytoplasmic (Potential).
TRANSMEM    246    266       Helical; (Potential).
TOPO_DOM    267    281       Extracellular (Potential).
TRANSMEM    282    302       Helical; (Potential).
TOPO_DOM    303    311       Cytoplasmic (Potential).
TRANSMEM    312    332       Helical; (Potential).
TOPO_DOM    333    337       Extracellular (Potential).
TRANSMEM    338    358       Helical; (Potential).
TOPO_DOM    359    372       Cytoplasmic (Potential).
TRANSMEM    373    393       Helical; (Potential).
TOPO_DOM    394    405       Extracellular (Potential).
TRANSMEM    406    426       Helical; (Potential).
TOPO_DOM    427    484       Cytoplasmic (Potential).
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Nucleotide Sequence
Length: 2422 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 484 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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