SG ID

SG00004951 

UniProt Accession

DDX5_MOUSE;Q61656;E9Q105;  

Theoretical PI

9.06  

Molecular Weight

69290 Da  

Genbank Nucleotide ID

X65627; AL603664;  

Genbank Protein ID

CAA46581.1; 

Gene Name

Ddx5 

Gene Synonyms/Alias

Tnz2 

Protein Name

Probable ATP-dependent RNA helicase DDX5 

Protein Synonyms/Alias

EC=3.6.4.13 DEAD box RNA helicase DEAD1;mDEAD1 DEAD box protein 5; RNA helicase p68; 

Mus musculus (Mouse) 

10090 

Uncertain 

Uncertain 

0.909156707 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.

Temporarily unavailable 

Abstract of related literatures

1. The complete cDNA coding for mouse P68 RNA helicase was cloned and its nucleotide sequence was determined. The sequence is about 95% identical to the human equivalent. Whereas the 5'-untranslated region is less conserved (71%), the 3'-ends of mouse and human mRNAs are nearly identical. Between stop codon and poly(A)-tail both sequences are 97% conserved. At the level of amino acid sequence, the similarity of both, mouse and human, DEAD box family proteins is as high as 98%. In situ hybridizations using cDNA subfragments as probes revealed a testis-selective expression of P68 RNA helicase mRNA. The signal was restricted to late pachytene spermatocytes and haploid spermatids. Northern blot analyses corroborated these results but suggested that expression of related mRNA species occurs in a variety of other tissues. PMID: [8445986] 

2. The mouse (Mus musculus) is the premier animal model for understanding human disease and development. Here we show that a comprehensive understanding of mouse biology is only possible with the availability of a finished, high-quality genome assembly. The finished clone-based assembly of the mouse strain C57BL/6J reported here has over 175,000 fewer gaps and over 139 Mb more of novel sequence, compared with the earlier MGSCv3 draft genome assembly. In a comprehensive analysis of this revised genome sequence, we are now able to define 20,210 protein-coding genes, over a thousand more than predicted in the human genome (19,042 genes). In addition, we identified 439 long, non-protein-coding RNAs with evidence for transcribed orthologs in human. We analyzed the complex and repetitive landscape of 267 Mb of sequence that was missing or misassembled in the previously published assembly, and we provide insights into the reasons for its resistance to sequencing and assembly by whole-genome shotgun approaches. Duplicated regions within newly assembled sequence tend to be of more recent ancestry than duplicates in the published draft, correcting our initial understanding of recent evolution on the mouse lineage. These duplicates appear to be largely composed of sequence regions containing transposable elements and duplicated protein-coding genes; of these, some may be fixed in the mouse population, but at least 40% of segmentally duplicated sequences are copy number variable even among laboratory mouse strains. Mouse lineage-specific regions contain 3,767 genes drawn mainly from rapidly-changing gene families associated with reproductive functions. The finished mouse genome assembly, therefore, greatly improves our understanding of rodent-specific biology and allows the delineation of ancestral biological functions that are shared with human from derived functions that are not. PMID: [19468303] 

3. The ability of macrophages to clear pathogens and elicit a sustained immune response is regulated by various cytokines, including interferon-gamma (IFN-gamma). To investigate the molecular mechanisms by which IFN-gamma modulates phagosome functions, we profiled the changes in composition, abundance, and phosphorylation of phagosome proteins in resting and activated macrophages by using quantitative proteomics and bioinformatics approaches. We identified 2415 phagosome proteins together with 2975 unique phosphorylation sites with a high level of sensitivity. Using network analyses, we determined that IFN-gamma delays phagosomal acquisition of lysosomal hydrolases and peptidases for the gain of antigen presentation. Furthermore, this gain in antigen presentation is dependent on phagosomal networks of the actin cytoskeleton and vesicle-trafficking proteins, as well as Src kinases and calpain proteases. Major histocompatibility complex class I antigen-presentation assays validated the molecular participation of these networks in the enhanced capacity of IFN-gamma-activated macrophages to crosspresent exogenous antigens to CD8(+) T cells. PMID: [19144319] 

Function

Involved in the alternative regulation of pre-mRNAsplicing; its RNA helicase activity is necessary for increasingtau exon 10 inclusion and occurs in a RBM4-dependent manner. Bindsto the tau pre-mRNA in the stem-loop region downstream of exon 10.The rate of ATP hydrolysis is highly stimulated by single-strandedRNA. Involved in transcriptional regulation; the function isindependent of the RNA helicase activity. Transcriptionalcoactivator for estrogen receptor ESR1 and androgen receptor AR.Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1and AF-2 domains transcriptional synergistic activity. Synergizeswith DDX17 and SRA1 RNA to activate MYOD1 transcriptional activityand involved in skeletal muscle differentiation. Transcriptionalcoactivator for p53/TP53 and involved in p53/TP53 transcriptionalresponse to DNA damage and p53/TP53-dependent apoptosis.Transcriptional coactivator for RUNX2 and involved in regulationof osteoblast differentiation. Acts as transcriptional repressorin a promoter-specicic manner; the function probbaly involvesassociation with histone deacetylases, such as HDAC1. 

Subcellular Location

Nucleus, nucleolus (By similarity). 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0005730	C:nucleolus	ISS:UniProtKB.
GO:0030529	C:ribonucleoprotein complex	ISO:MGI.
GO:0005681	C:spliceosomal complex	IEA:UniProtKB-KW.
GO:0005524	F:ATP binding	IEA:UniProtKB-KW.
GO:0008026	F:ATP-dependent helicase activity	IEA:InterPro.
GO:0003723	F:RNA binding	IEA:UniProtKB-KW.
GO:0003724	F:RNA helicase activity	ISS:UniProtKB.
GO:0003713	F:transcription coactivator activity	ISS:UniProtKB.
GO:0001701	P:in utero embryonic development	IMP:MGI.
GO:0006397	P:mRNA processing	IEA:UniProtKB-KW.
GO:0000122	P:negative regulation of transcription from RNA polymerase II promoter	ISS:UniProtKB.
GO:0043517	P:positive regulation of DNA damage response, signal transduction by p53 class mediator	ISS:UniProtKB.
GO:0045944	P:positive regulation of transcription from RNA polymerase II promoter	ISS:UniProtKB.
GO:0000381	P:regulation of alternative nuclear mRNA splicing, via spliceosome	ISS:UniProtKB.
GO:0060765	P:regulation of androgen receptor signaling pathway	ISS:UniProtKB.
GO:0045667	P:regulation of osteoblast differentiation	IMP:UniProtKB.
GO:2001014	P:regulation of skeletal muscle cell differentiation	IMP:UniProtKB.
GO:0008380	P:RNA splicing	IEA:UniProtKB-KW.
GO:0072332	P:signal transduction by p53 class mediator resulting in induction of apoptosis	ISS:UniProtKB.
GO:0006351	P:transcription, DNA-dependent	IEA:UniProtKB-KW.

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Interpro

IPR011545;    DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001;    Helicase_ATP-bd.
IPR001650;    Helicase_C.
IPR012587;    P68HR.
IPR000629;    RNA-helicase_DEAD-box_CS.
IPR014014;    RNA_helicase_DEAD_Q_motif.
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Pfam

PF00270;    DEAD;    1.
PF00271;    Helicase_C;    1.
PF08061;    P68HR;    2.
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SMART

SM00487;    DEXDc;    1.
SM00490;    HELICc;    1.
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PROSITE

PS00039;    DEAD_ATP_HELICASE;    1.
PS51192;    HELICASE_ATP_BIND_1;    1.
PS51194;    HELICASE_CTER;    1.
PS51195;    Q_MOTIF;    1.
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PRINTS

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Created Date

18-Oct-2012 

Record Type

GAS predicted 

Sequence Annotation

Nucleotide Sequence

Length: 2318 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 614 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
1433B_MOUSE	Ywhab	IntAct
AIRE_MOUSE	Aire	IntAct
BTK_MOUSE	Btk	MINT
BTK_MOUSE	Btk	IntAct
IKKB_MOUSE	Ikbkb	IntAct
TIAR_MOUSE	Tial1	MINT

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Click to view expression 

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