Tag Content
SG ID
SG00005138 
UniProt Accession
Theoretical PI
6.34  
Molecular Weight
27372 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Psma6 
Gene Synonyms/Alias
 
Protein Name
Proteasome subunit alpha type-6 
Protein Synonyms/Alias
EC=3.4.25.1 Macropain iota chain; Multicatalytic endopeptidase complex iota chain; Proteasome iota chain; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:12;56485209-56519441;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.907329085 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. The proteasome is a large multicatalytic proteinase that plays a role in the generation of peptides for presentation by major histocompatibility complex class I molecules. The 20S proteolytic core of mammalian proteasomes is assembled from a group of 17 protein subunits that generate a distinctive pattern of spots upon two-dimensional gel electrophoresis. The genes for most of these subunits have been cloned from humans and rats. We isolated cDNA clones for the mouse orthologues of ten of the subunits [PSMA1 (C2), PSMA2 (C3), PSMA3 (C8), PSMA4 (C9), PSMA5 (ZETA), PSMA6 (IOTA), PSMA7 (C6-I), PSMB2 (C7-I), PSMB3 (C10-II), and PSMB5 (X)] to complete the cloning of all of the mouse subunits. Using antisera raised against these subunits or their orthologues, we verified the identity of these proteins by two-dimensional NEPHGE-PAGE. PMID: [10436176] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

4. Chronic infection of hepatitis virus B (HBV) has been proven to be one of the most important risk factors of hepatocellular carcinoma (HCC). HBx has been shown to function in the viral life cycle and the development of HCC. Recently, we have reported that HBx transgenic mice (p21-HBx), generated by gene knockin, develop HCC at the age of 18 months. To further study the function of HBx during the development of HCC in vivo, we performed proteomic analysis of the transgenic and wild-type control mice. The combination of 2-DE and MALDI-TOF MS revealed that proteasome subunits (PSMA6, PSMB4, PSMC2 and PSMD12) were up-regulated in tumor tissues of the p21-HBx transgenic mice. Cathepsin B, ubiquinol-cytochrome C reductase core protein 1 and an ATP-dependent caseinolytic protease, which were involved in the cellular proteolytic process, were also found increased in tumors. The results were confirmed in tumors of transgenic mice and HCCs of human using RT-PCR. All these results suggested that the strengthened ubiquitin-proteasome and lysosomal pathway might contribute to the development of HBx-related HCC. PMID: [16317774] 

5. Metazoans employ reversible tyrosine phosphorylation to regulate innumerable biological processes. Thus, the large-scale identification of tyrosine phosphorylation sites from primary tissues is an essential step toward a molecular systems understanding of dynamic regulation in vivo. The relative paucity of phosphotyrosine has greatly limited its identification in large-scale phosphoproteomic experiments. However, using antiphosphotyrosine peptide immunoprecipitations, we report the largest study to date of tyrosine phosphorylation sites from primary tissue, identifying 414 unique tyrosine phosphorylation sites from murine brain. To measure the conservation of phosphorylated tyrosines and their surrounding residues, we constructed a computational pipeline and identified patterns of conservation within the signature of phosphotyrosine. PMID: [18034455] 

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Function
The proteasome is a multicatalytic proteinase complexwhich is characterized by its ability to cleave peptides with Arg,Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral orslightly basic pH. The proteasome has an ATP-dependent proteolyticactivity. 
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Subcellular Location
Cytoplasm. Nucleus. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005739 C:mitochondrion IEA:Compara.
GO:0016363 C:nuclear matrix IDA:BHF-UCL.
GO:0005844 C:polysome ISS:BHF-UCL.
GO:0019773 C:proteasome core complex, alpha-subunit complex ISS:BHF-UCL.
GO:0030017 C:sarcomere ISS:BHF-UCL.
GO:0003723 F:RNA binding IDA:BHF-UCL.
GO:0004298 F:threonine-type endopeptidase activity IEA:UniProtKB-KW.
GO:0051092 P:positive regulation of NF-kappaB transcription factor activity ISS:BHF-UCL.
GO:0051603 P:proteolysis involved in cellular protein catabolic process ISS:BHF-UCL.
GO:0007519 P:skeletal muscle tissue development NAS:BHF-UCL.
GO:0006511 P:ubiquitin-dependent protein catabolic process IEA:InterPro.
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Interpro
IPR000426;    Proteasome_asu_N.
IPR023332;    Proteasome_suA-type.
IPR001353;    Proteasome_sua/b.
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Pfam
PF00227;    Proteasome;    1.
PF10584;    Proteasome_A_N;    1.
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SMART
SM00948;    Proteasome_A_N;    1.
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PROSITE
PS00388;    PROTEASOME_A_1;    1.
PS51475;    PROTEASOME_A_2;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    246       Proteasome subunit alpha type-6.
                             /FTId=PRO_0000124131.
MOD_RES     102    102       N6-acetyllysine (By similarity).
MOD_RES     104    104       N6-acetyllysine (By similarity).
MOD_RES     159    159       Phosphotyrosine.
MOD_RES     160    160       Phosphotyrosine (By similarity).
CROSSLNK     59     59       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CONFLICT    133    133       P -> T (in Ref. 3; BAE36518).
TURN         10     12
HELIX        23     32
HELIX        33     35
STRAND       38     43
STRAND       45     53
TURN         63     65
STRAND       69     71
STRAND       73     82
HELIX        84    105
HELIX       111    127
STRAND      128    131
STRAND      135    144
TURN        145    147
STRAND      148    154
STRAND      156    158
STRAND      160    169
HELIX       172    184
HELIX       191    206
TURN        212    214
STRAND      215    223
HELIX       232    242
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Nucleotide Sequence
Length: 945 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 246 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Ppp2r1aIntAct 
Psma1IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
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