Tag Content
UniProt Accession
Theoretical PI
Molecular Weight
35381 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gene Synonyms/Alias
Protein Name
PHD finger protein 7 
Protein Synonyms/Alias
Testis development protein NYD-SP6; 
Mus musculus (Mouse) 
NCBI Taxonomy ID
Chromosome Location
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Probability (GAS) of Function in Spermatogenesis
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Temporarily unavailable 
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

3. Using cDNA microarray hybridization from a human testicular cDNA library, a novel gene exhibiting 30-fold difference in expression level between adult and embryo human testes was cloned and named NYD-SP6, which was 1858 bp in length with 87% nucleotide identity with the mouse homologue sequence. The deduced protein structure of NYD-SP6 was found to contain two plant homeodomain (PHD) finger domains, believed to be involved in activating transcriptional regulation. Tissue distribution analysis using Northern blot indicated that the NYD-SP6 gene was expressed in a wide range of tissues, with a high expression level in the testis. Its expression in human and mouse testes by in situ hybridization was confined to Sertoli cells and the expression was developmentally regulated as demonstrated by cDNA microarray, in situ hybridization, and semiquantitative PCR in mouse testes. GFP/NYD-SP6 protein was predominantly localized in the nucleus of the transfected CHO cells, indicating its role in transcriptional regulation. In contrast, the N-terminal truncated NYD-SP6 (tNYD-SP6) localized in the nuclear envelope, indicating this region function as a nuclear localization signal. Further Northern blot analysis of gene expression in patients with spermatogenesis arrest revealed that NYD-SP6 was absent in one patient whose spermatogenesis was blocked at the stage of spermatocytes. Taken together, these results suggest that the putative protein of NYD-SP6 may play an important role in stimulating transcription involved in testicular development and/or spermatogenesis. PMID: [11829468] 

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May play a role in spermatogenesis. 
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Subcellular Location
Nucleus (By similarity). 
Tissue Specificity
Highly expressed in Sertoli cells in testis. 
Gene Ontology
GO IDGO termEvidence
GO:0005794 C:Golgi apparatus IEA:Compara.
GO:0005634 C:nucleus IEA:UniProtKB-SubCell.
GO:0005886 C:plasma membrane IEA:Compara.
GO:0008270 F:zinc ion binding IEA:InterPro.
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IPR011011;    Znf_FYVE_PHD.
IPR001965;    Znf_PHD.
IPR001841;    Znf_RING.
IPR013083;    Znf_RING/FYVE/PHD.
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SM00249;    PHD;    2.
SM00184;    RING;    2.
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PS01359;    ZF_PHD_1;    FALSE_NEG.
PS50016;    ZF_PHD_2;    FALSE_NEG.
PS00518;    ZF_RING_1;    FALSE_NEG.
PS50089;    ZF_RING_2;    FALSE_NEG.
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Created Date
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    307       PHD finger protein 7.
ZN_FING      97    145       PHD-type; atypical.
ZN_FING     160    208       RING-type; degenerate.
VAR_SEQ     228    266       Missing (in isoform 2).
STRAND      263    266
STRAND      274    276
HELIX       284    286
TURN        298    300
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Nucleotide Sequence
Length: 1984 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 307 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data