SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00005737 
UniProt Accession
Theoretical PI
4.98  
Molecular Weight
36745 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Pbk 
Gene Synonyms/Alias
Topk 
Protein Name
Lymphokine-activated killer T-cell-originated protein kinase 
Protein Synonyms/Alias
EC=2.7.12.2 PDZ-binding kinase; T-LAK cell-originated protein kinase; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:14;66424674-66436659;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.749018931 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. A novel protein kinase, TOPK (T-LAK cell-originated protein kinase), was isolated from a lymphokine-activated killer T (T-LAK) cell subtraction cDNA fragment library. The open reading frame of the TOPK gene encodes a protein of 322 amino acids, possessing a protein kinase domain profile. The cap site analysis of the 5'-end of TOPK mRNA revealed two forms, a major full-length form and a minor spliced form at the 5'-site, both encoding the same protein. A BLAST homology search and phylogenetic analysis indicated that TOPK is related to dual specific mitogen-activated protein kinase kinase (MAPKK). The transfection of the TOPK gene to COS-7 cells up-regulated a phosphorylation of p38 MAPK but not ERK1/2 or SAPK/JNK. Gel precipitation study indicated that TOPK protein can be associated with p38 in vitro. Tissue distribution of TOPK mRNA expression was specific for the testis, T-LAK cells, activated lymphoid cells, and lymphoid tumors. On the other hand, deactivated T-LAK cells did not show TOPK mRNA expression. These data suggest that TOPK is a newly identified member of a novel MEK3/6-related MAPKK that may be enrolled in the activation of lymphoid cells and support testicular functions. PMID: [10781613] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
Phosphorylates MAP kinase p38. Seems to be active onlyin mitosis. May also play a role in the activation of lymphoidcells. When phosphorylated, forms a complex with TP53, leading toTP53 destabilization (By similarity). 
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Subcellular Location
 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0004674 F:protein serine/threonine kinase activity ISO:MGI.
GO:0018105 P:peptidyl-serine phosphorylation ISO:MGI.
GO:0018107 P:peptidyl-threonine phosphorylation ISO:MGI.
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Interpro
IPR011009;    Kinase-like_dom.
IPR000719;    Prot_kinase_cat_dom.
IPR008271;    Ser/Thr_kinase_AS.
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Pfam
PF00069;    Pkinase;    1.
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SMART
PROSITE
PS00107;    PROTEIN_KINASE_ATP;    FALSE_NEG.
PS50011;    PROTEIN_KINASE_DOM;    1.
PS00108;    PROTEIN_KINASE_ST;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    330       Lymphokine-activated killer T-cell-
                             originated protein kinase.
                             /FTId=PRO_0000086764.
DOMAIN       31    330       Protein kinase.
NP_BIND      37     45       ATP (By similarity).
ACT_SITE    166    166       Proton acceptor (By similarity).
BINDING      63     63       ATP (By similarity).
MOD_RES       1      1       N-acetylmethionine (By similarity).
MOD_RES       9      9       Phosphothreonine (By similarity).
MOD_RES      23     23       Phosphothreonine (By similarity).
MOD_RES      31     31       Phosphoserine (By similarity).
MOD_RES      58     58       Phosphoserine (By similarity).
MOD_RES     199    199       Phosphothreonine (By similarity).
CONFLICT    123    123       N -> D (in Ref. 2; BAB23029).
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Nucleotide Sequence
Length: 1666 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 330 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
SMAD4BioGRID 
SMAD4MINT 
Other Protein-Protein interaction resources
String database  
View Microarray data
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