Tag Content
UniProt Accession
Theoretical PI
Molecular Weight
70671 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gene Synonyms/Alias
Protein Name
Polyadenylate-binding protein 1 
Protein Synonyms/Alias
PABP-1Poly(A)-binding protein 1 
Mus musculus (Mouse) 
NCBI Taxonomy ID
Chromosome Location
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Probability (GAS) of Function in Spermatogenesis
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Temporarily unavailable 
Abstract of related literatures

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

4. In male germ cells many mRNAs are sequestered by proteins into translationally silent messenger ribo-nucleoprotein (mRNP) particles. These masked paternal mRNAs are stored and translated at specific times of germ cell development. Little is known about the mammalian testicular mRNA masking proteins bound to non-polysomal mRNAs. In this report, the major proteins binding to non-polysomal testicular mRNAs were isolated and analyzed. The two predominant proteins identified were: a Y-box protein (MSY2), the mammalian homolog to the Xenopus oocyte masking protein FRGY2/mRNP3+4, and a poly(A) binding protein. A kinase activity was also found associated with these non-polysomal RNAs. The kinase co-immunoprecipitates with MSY2 and phosphorylates MSY2 in vitro. The MSY2 associated kinase is not casein kinase 2, the kinase believed to phosphorylate mRNP3+4 in oocytes, but a yet unidentified kinase. MSY2 was found to be phosphorylated in vivo and MSY2 dephosphorylation led to a decrease in its affinity to bind RNA as judged by northwestern blotting. Therefore, testicular masked mRNAs may be regulated by the phosphorylation state of MSY2. Reconstitution experiments in which non-polysomal mRNA-binding proteins are dissociated from their RNAs and allowed to bind to exogenous mRNAs suggest that MSY2 binds RNA in a sequence-independent fashion. Furthermore, association of the non-polysomal derived proteins to exogenous non-specific mRNAs led to their translational repression in vitro. PMID: [10076007] 

5. Messenger RNA decay mediated by the c-fos major protein coding-region determinant of instability (mCRD) is a useful system for studying translationally coupled mRNA turnover. Among the five mCRD-associated proteins identified previously, UNR was found to be an mCRD-binding protein and also a PABP-interacting protein. Interaction between UNR and PABP is necessary for the full destabilization function of the mCRD. By testing different classes of mammalian poly(A) nucleases, we identified CCR4 as a poly(A) nuclease involved in the mCRD-mediated rapid deadenylation in vivo and also associated with UNR. Blocking either translation initiation or elongation greatly impeded poly(A) shortening and mRNA decay mediated by the mCRD, demonstrating that the deadenylation step is coupled to ongoing translation of the message. These findings suggest a model in which the mCRD/UNR complex serves as a "landing/assembly" platform for formation of a deadenylation/decay mRNA-protein complex on an mCRD-containing transcript. The complex is dormant prior to translation. Accelerated deadenylation and decay of the transcript follows ribosome transit through the mCRD. This study provides new insights into a mechanism by which interplay between mRNA turnover and translation determines the lifespan of an mCRD-containing mRNA in the cytoplasm. PMID: [15314026] 

6. The elongation of poly(A) tails in cytoplasm is essential for oogenesis and early embryogenesis in Xenopus laevis. mGLD-2 is a mouse homologue of Xenopus cytoplasmic poly(A) polymerase xGLD-2. We found an association of mGLD-2 with cytoplasmic polyadenylation components, CPEB and CPSF described in Xenopus oocytes. To clarify the role of mGLD-2 in mouse, we produced an mGLD-2 disrupted mouse line by homologous recombination. In spite of the ubiquitous expression of mGLD-2, the disrupted mice were apparently normal and healthy. Moreover, it was demonstrated that mGLD-2 disruption did not affect the poly(A) tail elongation in oocytes using reporter RNAs. Coincide with these observations, the maturation of the oocytes was normal and the mice were fertile. Thus mGLD-2 is dispensable for full-term development and oogenesis. Our results also indicate that there is another source of cytoplasmic poly(A) polymerase in mouse. PMID: [17927953] 

7. Mast cells play a central role in type I hypersensitivity reactions and allergic disorders such as anaphylaxis and asthma. Activation of mast cells, through a cascade of phosphorylation events, leads to the release of mediators of the early phase allergic response. Understanding the molecular architecture underlying mast cell signaling may provide possibilities for therapeutic intervention in asthma and other allergic diseases. Although many details of mast cell signaling have been described previously, a systematic, quantitative analysis of the global tyrosine phosphorylation events that are triggered by activation of the mast cell receptor is lacking. In many cases, the involvement of particular proteins in mast cell signaling has been established generally, but the precise molecular mechanism of the interaction between known signaling proteins often mediated through phosphorylation is still obscure. Using recently advanced methodologies in mass spectrometry, including automation of phosphopeptide enrichments and detection, we have now substantially characterized, with temporal resolution as short as 10 s, the sites and levels of tyrosine phosphorylation across 10 min of FcepsilonRI-induced mast cell activation. These results reveal a far more extensive array of tyrosine phosphorylation events than previously known, including novel phosphorylation sites on canonical mast cell signaling molecules, as well as unexpected pathway components downstream of FcepsilonRI activation. Furthermore, our results, for the first time in mast cells, reveal the sequence of phosphorylation events for 171 modification sites across 121 proteins in the MCP5 mouse mast cell line and 179 modification sites on 117 proteins in mouse bone marrow-derived mast cells. PMID: [17947660] 

8. Mouse spermatogenic cells are known to contain at least two isoforms of cytoplasmic poly(A)-binding proteins, PABPC1 and PABPC2 (previously known as PABPT). In this study, we have characterized PABPC1 and PABPC2. PABPC2 was present in pachytene spermatocytes and round spermatids, whereas elongating spermatids still included PABPC1. These two proteins are capable of binding mRNA poly(A) tails nonspecifically and of directly associating with each other and with several translational regulators, including EIF4G1, PAIP1, PAIP2, and PIWIL1 (previously known as MIWI). Moreover, both PABPC1 and PABPC2 exhibited the ability to enhance translation of a reporter mRNA in vitro. Despite these similarities, PABPC2 was distinguished from PABPC1 by the absence of PABPC2 in actively translating polyribosomes of testicular cells. PABPC1 was distributed in polyribosomes and in translationally inactive messenger ribonucleoprotein particles. Most importantly, PABPC2 and PIWIL1 were noticeably enriched in the chromatoid body of round spermatids. These results suggest that PABPC2 may function in translational repression during spermatogenesis. PMID: [19020299] 

Back to Top
Binds the poly(A) tail of mRNA. May be involved incytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulationcan either be enhanced by PAIP1 or repressed by PAIP2. Canprobably bind to cytoplasmic RNA sequences other than poly(A) invivo. Involved in translationally coupled mRNA turnover.Implicated with other RNA-binding proteins in the cytoplasmicdeadenylation/translational and decay interplay of the FOS mRNAmediated by the major coding-region determinant of instability(mCRD) domain. Involved in regulation of nonsense-mediated decay(NMD) of mRNAs containing premature stop codons; for therecognition of premature termination codons (PTC) and initiationof NMD a competitive interaction between UPF1 and PABPC1 with theribosome-bound release factors is proposed (By similarity). 
Back to Top
Subcellular Location
Cytoplasm (By similarity). Nucleus (Bysimilarity). Note=Localized in cytoplasmic mRNP granulescontaining untranslated mRNAs. Shuttles between the cytoplasm andthe nucleus (By similarity). 
Tissue Specificity
Gene Ontology
GO IDGO termEvidence
GO:0010494 C:cytoplasmic stress granule ISS:UniProtKB.
GO:0005681 C:spliceosomal complex IEA:UniProtKB-KW.
GO:0000166 F:nucleotide binding IEA:InterPro.
GO:0008143 F:poly(A) RNA binding ISS:UniProtKB.
GO:0006397 P:mRNA processing IEA:UniProtKB-KW.
GO:2000623 P:negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay ISS:UniProtKB.
GO:0000184 P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay IEA:UniProtKB-KW.
GO:0008380 P:RNA splicing IEA:UniProtKB-KW.
Back to Top
IPR012677;    Nucleotide-bd_a/b_plait.
IPR006515;    PABP_1234.
IPR002004;    PABP_HYD.
IPR000504;    RRM_dom.
Back to Top
PF00658;    PABP;    1.
PF00076;    RRM_1;    4.
Back to Top
SM00517;    PolyA;    1.
SM00360;    RRM;    4.
Back to Top
PS51309;    PABC;    1.
PS50102;    RRM;    4.
Back to Top
Created Date
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    636       Polyadenylate-binding protein 1.
DOMAIN       11     89       RRM 1.
DOMAIN       99    175       RRM 2.
DOMAIN      191    268       RRM 3.
DOMAIN      294    370       RRM 4.
DOMAIN      542    619       PABC.
REGION      166    289       CSDE1-binding (By similarity).
COMPBIAS    495    501       Poly-Ala.
MOD_RES       1      1       N-acetylmethionine (By similarity).
MOD_RES      96     96       Phosphoserine (By similarity).
MOD_RES     116    116       Phosphotyrosine.
MOD_RES     299    299       N6-methyllysine (By similarity).
MOD_RES     315    315       Phosphoserine (By similarity).
MOD_RES     319    319       Phosphothreonine (By similarity).
MOD_RES     342    342       Phosphoserine (By similarity).
MOD_RES     364    364       Phosphotyrosine (By similarity).
MOD_RES     455    455       Omega-N-methylated arginine; by CARM1 (By
MOD_RES     460    460       Omega-N-methylated arginine; by CARM1 (By
MOD_RES     493    493       Dimethylated arginine; alternate (By
MOD_RES     493    493       Omega-N-methylarginine; alternate (By
MOD_RES     512    512       N6-acetyllysine (By similarity).
CONFLICT    176    176       R -> Q (in Ref. 1; CAA46522).
Back to Top
Nucleotide Sequence
Length: 2244 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 636 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data