Tag Content
UniProt Accession
Theoretical PI
Molecular Weight
62724 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gene Synonyms/Alias
Protein Name
Tubby-related protein 2 
Protein Synonyms/Alias
Protein P4-6; Tubby-like protein 2; 
Mus musculus (Mouse) 
NCBI Taxonomy ID
Chromosome Location
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Probability (GAS) of Function in Spermatogenesis
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Temporarily unavailable 
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

3. A cDNA for a rat brain phosphodiesterase (PDE) was used to screen a mouse testis library to identify the murine PDEs which are expressed in this tissue. A clone of 981 bp, p4-6, was isolated and shown to exhibit limited identity at the amino acid level to the rat brain PDE (20%). The putative protein encoded by clone p4-6 also contains multiple potential modification sites, for phosphorylation, myristylation, and glycosylation, many of which are located at positions similar to those found for rat brain PDE. The gene identified by p4-6 yields 3 transcripts, an abundant 1.9 kb transcript, and less abundant transcripts of 3.8 and 6.7 kb. Of the nine tissues examined in this study, the expression of the corresponding gene was limited to the adult mouse testis. Furthermore, the expression in the testis was most abundant in the germ cell lineage, although low levels were detected in somatic cells of the testis as well. Analysis of RNA from testes at different stages of development suggested that the p4-6 gene is most abundantly expressed in germ cells that have completed the meiotic divisions. PMID: [7509194] 

4. Tubby-like proteins (Tulps) with no signal peptide have been characterized as cytoplasmic proteins with various intracellular functions, including binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P(2)]. PI(4,5)P(2) has been implicated in unconventional secretion of fibroblast growth factor-2 without a signal peptide. Here, we show that all Tulps are expressed intracellularly and extracellularly. Tubby secretion is partially dependent on its PI(4,5)P(2)-binding activity with an essential secretory signal in the N-terminus. Pathogenic mutation in Tubby mice has no impact on tubby extracellular trafficking. Moreover, unconventional secretion of tubby and Tulp1 is independent of endoplasmic reticulum-Golgi pathway. These data implicate that Tulps may function extracellularly as well. PMID: [19695251] 

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Subcellular Location
Cytoplasm. Secreted. Note=Does not have acleavable signal peptide and is secreted by a non-conventionalpathway. 
Tissue Specificity
Expressed in retina and testis. 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IEA:UniProtKB-SubCell.
GO:0005576 C:extracellular region IEA:UniProtKB-SubCell.
GO:0008081 F:phosphoric diester hydrolase activity ISS:MGI.
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IPR000007;    Tubby_C.
IPR025659;    Tubby_C-like.
IPR018066;    Tubby_C_CS.
IPR005398;    Tubby_N.
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PF01167;    Tub;    1.
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PS01200;    TUB_1;    1.
PS01201;    TUB_2;    FALSE_NEG.
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PR01573;    SUPERTUBBY.;   
PR01574;    TUBBYPROTEIN.;   
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Created Date
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    562       Tubby-related protein 2.
VAR_SEQ       1    112       Missing (in isoform 3, isoform 4 and
                             isoform 5).
VAR_SEQ     121    132       Missing (in isoform 4).
VAR_SEQ     561    562       FF -> CE (in isoform 2, isoform 3 and
                             isoform 4).
VAR_SEQ     561    562       Missing (in isoform 5).
CONFLICT      1      1       M -> R (in Ref. 1; AAD38452).
CONFLICT    314    314       L -> V (in Ref. 4; CAA49481).
CONFLICT    324    324       C -> W (in Ref. 1; AAD38452 and 4;
CONFLICT    347    347       A -> G (in Ref. 1; AAD38452 and 4;
CONFLICT    504    504       T -> S (in Ref. 4; CAA49481).
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Nucleotide Sequence
Length: 1744 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 562 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data