SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00006662 
UniProt Accession
Theoretical PI
5.31  
Molecular Weight
27285 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Ndufv2 
Gene Synonyms/Alias
 
Protein Name
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial 
Protein Synonyms/Alias
EC=1.6.5.3EC=1.6.99.3 NADH-ubiquinone oxidoreductase 24 kDa subunit;Flags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:17;66428135-66450899;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.729861092 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
Core subunit of the mitochondrial membrane respiratorychain NADH dehydrogenase (Complex I) that is believed to belong tothe minimal assembly required for catalysis. Complex I functionsin the transfer of electrons from NADH to the respiratory chain.The immediate electron acceptor for the enzyme is believed to beubiquinone (By similarity). 
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Subcellular Location
Mitochondrion inner membrane (Bysimilarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005747 C:mitochondrial respiratory chain complex I IBA:RefGenome.
GO:0051537 F:2 iron, 2 sulfur cluster binding IEA:UniProtKB-KW.
GO:0046872 F:metal ion binding IEA:UniProtKB-KW.
GO:0051287 F:NAD binding IEA:InterPro.
GO:0008137 F:NADH dehydrogenase (ubiquinone) activity IBA:RefGenome.
GO:0048738 P:cardiac muscle tissue development IEA:Compara.
GO:0006120 P:mitochondrial electron transport, NADH to ubiquinone IBA:RefGenome.
GO:0007399 P:nervous system development IEA:Compara.
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Interpro
IPR002023;    NADH_UbQ_OxRdtase_24kDa_su.
IPR012336;    Thioredoxin-like_fold.
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Pfam
PF01257;    Complex1_24kDa;    1.
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SMART
PROSITE
PS01099;    COMPLEX1_24K;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
TRANSIT       1     31       Mitochondrion.
CHAIN        32    248       NADH dehydrogenase [ubiquinone]
                             flavoprotein 2, mitochondrial.
                             /FTId=PRO_0000020004.
METAL       134    134       Iron-sulfur (2Fe-2S) (Potential).
METAL       139    139       Iron-sulfur (2Fe-2S) (Potential).
METAL       175    175       Iron-sulfur (2Fe-2S) (Potential).
METAL       179    179       Iron-sulfur (2Fe-2S) (Potential).
VAR_SEQ       1     96       Missing (in isoform 2).
                             /FTId=VSP_025017.
CONFLICT     64     64       A -> T (in Ref. 1; BAB28888).
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Nucleotide Sequence
Length: 845 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 248 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
YwhabIntAct 
Poldip2String 
Grin2bIntAct 
Poldip2String 
SNCABioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
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