SG ID

SG00006853 

UniProt Accession

CABYR_MOUSE;Q9D424;Q91Y41;Q91Y42;  

Theoretical PI

4.48  

Molecular Weight

48333 Da  

Genbank Nucleotide ID

AF359382; AF359383; AF359384; AF359385; AK016856;  

Genbank Protein ID

AAK49987.1; AAK49988.1; AAK49989.1; AAK49990.1; BAB30467.1; 

Gene Name

Cabyr 

Gene Synonyms/Alias

Cbp86 

Protein Name

Calcium-binding tyrosine phosphorylation-regulated protein 

Protein Synonyms/Alias

Calcium-binding protein 86; Testis-specific calcium-binding protein CBP86; 

Mus musculus (Mouse) 

10090 

Uncertain 

Uncertain 

0.586719578 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.

Temporarily unavailable 

Abstract of related literatures

1. Human calcium-binding tyrosine-phosphorylation regulated protein (CABYR) is a polymorphic, testis-specific, calcium binding protein that undergoes tyrosine phosphorylation during in vitro capacitation. A protein kinase A (PKA) regulatory subunit type II alpha (RII-alpha) homologous domain in the N-terminus, phosphorylation dependent Ca(++) binding isoforms, and localization to the principal piece of the human sperm tail suggest that CABYR may be involved in sperm motility. In this paper, four mouse orthologous cDNAs and the genomic DNA of CABYR were cloned, nucleotide and protein sequences of mouse and humans were compared, and the genomic organization of the mCABYR gene was analyzed. Human and mouse CABYR conserve potential functional motifs including a domain homologous to the dimerization interface of cyclic adenosine monophosphate dependent PKA RII-alpha, 14 PXXP motifs, and regions of homology with extensins and src homology-3-binding protein 1. mCABYR is arranged into six exons spanning about 14 kb of DNA. Mouse CABYR showed several similarities with human CABYR: (1) the protein was localized to the principal piece of mouse epididymal spermatozoa; (2) mouse CABYR has two coding regions (CR-A and CR-B), with 66 and 82% identity, respectively to human; and (3) mCABYR showed the presence of two testis-specific transcripts of approximately 1.4 and approximately 2.4 kb. Three murine splice variants were identified, two of which spliced into CR-B. Exon 4, present in all human and mouse variants and comprising 85% of CR-A appears suitable for targeted deletion. The overall 81% nucleotide identity between mouse and human CABYR, the common genomic organization, presence of similar testis-specific transcripts, localization in the principal piece of tail and occurrence of homologous splice variants indicate an authentic murine orthologue of CABYR has been identified. PMID: [12801634] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. We report characterization of a novel testis- and sperm-specific protein, FSCB (fibrous sheath CABYR binding), that is expressed post-meiotically and localized in mouse sperm flagella. FSCB was identified as a binding partner of CABYR, a calcium-binding protein that is tyrosine-phosphorylated during capacitation. Orthologous genes of FSCB are present in other mammals, including rat and human, and conserved motifs in FSCB include PXXP, proline-rich and extensin-like regions. FSCB is phosphorylated by protein kinase A as shown by in vitro phosphorylation assay and also by determining phosphorylation sites in native FSCB from mouse sperm. Calcium overlay assay showed that FSCB is a calcium-binding protein from sperm. FSCB is a post meiotic protein first expressed at step 11 of mouse spermatogenesis in the elongating spermatids, and it subsequently incorporates into the flagellar principal piece of the sperm. Ultrastructurally, FSCB localized to a cortical layer of intermediate electron density at the surface of the ribs and longitudinal columns of the fibrous sheath. Due to its temporal appearance during spermiogenesis and location at the cortex of the fibrous sheath, FSCB is postulated to be involved in the later stages of fibrous sheath assembly. PMID: [17855365] 

Function

May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosomereaction. May bind calcium in vitro (By similarity). 

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Cellprojection, cilium, flagellum. Note=Localizes to fibrous sheathincluding the surface of the longitudinal columns and ribs of theprincipal piece of sperm flagella. 

Tissue Specificity

Expressed in spermatozoa. 

Gene Ontology

GO ID	GO term	Evidence
GO:0005737	C:cytoplasm	IDA:BHF-UCL.
GO:0005856	C:cytoskeleton	IEA:UniProtKB-SubCell.
GO:0035686	C:flagellar fibrous sheath	IDA:BHF-UCL.
GO:0031514	C:motile cilium	ISS:BHF-UCL.
GO:0005509	F:calcium ion binding	IDA:MGI.
GO:0008603	F:cAMP-dependent protein kinase regulator activity	IEA:InterPro.
GO:0019722	P:calcium-mediated signaling	NAS:BHF-UCL.
GO:0001539	P:ciliary or flagellar motility	ISS:BHF-UCL.
GO:0051259	P:protein oligomerization	NAS:BHF-UCL.
GO:0048240	P:sperm capacitation	NAS:BHF-UCL.

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Interpro

IPR003117;    cAMP_dep_PK_reg_su_I/II_a/b.
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Pfam

PF02197;    RIIa;    1.
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SMART

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PROSITE

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PRINTS

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Created Date

18-Oct-2012 

Record Type

GAS predicted 

Sequence Annotation

Nucleotide Sequence

Length: 2125 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 453 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt

Gene Symbol

Ref Databases

Other Protein-Protein interaction resources

String database  

UniProt	Gene Symbol	Ref Databases
AKAP1_MOUSE	Akap1	String
AKAP3_MOUSE	Akap3	String
ARRC_MOUSE	Arr3	String
ASPM_MOUSE	Aspm	String
CAB45_MOUSE	Sdf4	String
AKAP1_MOUSE	Akap1	String
AKAP3_MOUSE	Akap3	String
ARRC_MOUSE	Arr3	String
ASPM_MOUSE	Aspm	String
CAB45_MOUSE	Sdf4	String
DMRT3_MOUSE	Dmrt3	String
MSH6_MOUSE	Msh6	String
ROP1_MOUSE	Ropn1	String
RPIA_MOUSE	Rpia	String
SP17_MOUSE	Spa17	String
SRC_MOUSE	Src	String
DMRT3_MOUSE	Dmrt3	String
MSH6_MOUSE	Msh6	String
ROP1_MOUSE	Ropn1	String
RPIA_MOUSE	Rpia	String
SP17_MOUSE	Spa17	String
SRC_MOUSE	Src	String

View Microarray data

Click to view expression 

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