SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00006865 
UniProt Accession
Theoretical PI
6.26  
Molecular Weight
65616 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Ik 
Gene Synonyms/Alias
Red, Rer 
Protein Name
Protein Red 
Protein Synonyms/Alias
Cytokine IK; IK factor; Protein RER; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:18;36904310-36917293;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.662289509 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. A novel human gene RED, and the murine homologue, MuRED, were cloned. These genes were named after the extensive stretch of alternating arginine (R) and glutamic acid (E) or aspartic acid (D) residues that they contain. We term this the 'RED' repeat. The genes of both species were expressed in a wide range of tissues and we have mapped the human gene to chromosome 5q22-24. MuRED and RED shared 98% sequence identity at the amino acid level. The open reading frame of both genes encodes a 557 amino acid protein. RED fused to a fluorescent tag was expressed in nuclei of transfected cells and localised to nuclear dots. Co-localisation studies showed that these nuclear dots did not contain either PML or Coilin, which are commonly found in the POD or coiled body nuclear compartments. Deletion of the amino terminal 265 amino acids resulted in a failure to sort efficiently to the nucleus, though nuclear dots were formed. Deletion of a further 50 amino acids from the amino terminus generates a protein that can sort to the nucleus but is unable to generate nuclear dots. Neither construct localised to the nucleolus. The characteristics of RED and its nuclear localisation implicate it as a regulatory protein, possibly involved in transcription. PMID: [10216252] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
May bind to chromatin. 
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Subcellular Location
Nucleus. 
Tissue Specificity
Ubiquitous. 
Gene Ontology
GO IDGO termEvidence
GO:0005634 C:nucleus IEA:UniProtKB-SubCell.
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Interpro
IPR012492;    RED_C.
IPR012916;    RED_N.
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Pfam
PF07807;    RED_C;    1.
PF07808;    RED_N;    1.
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SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    557       Protein Red.
                             /FTId=PRO_0000097236.
REPEAT      342    343       1.
REPEAT      344    345       2.
REPEAT      346    347       3.
REPEAT      348    349       4.
REPEAT      350    351       5.
REPEAT      352    353       6.
REPEAT      354    355       7.
REPEAT      356    357       8.
REPEAT      358    359       9.
REPEAT      360    361       10.
REPEAT      362    363       11.
REPEAT      364    365       12.
REPEAT      366    367       13.
REPEAT      368    369       14.
REPEAT      370    371       15.
REPEAT      372    373       16.
REPEAT      374    375       17.
REGION      342    375       17 X 2 AA tandem repeats of R-[ED].
COMPBIAS    182    187       Poly-Glu.
MOD_RES      83     83       N6-acetyllysine (By similarity).
MOD_RES      98     98       N6-acetyllysine (By similarity).
MOD_RES     386    386       N6-acetyllysine (By similarity).
MOD_RES     408    408       N6-acetyllysine (By similarity).
MOD_RES     460    460       Phosphoserine (By similarity).
MOD_RES     485    485       Phosphothreonine (By similarity).
CONFLICT     91     91       R -> G (in Ref. 1; CAA06880).
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Nucleotide Sequence
Length: 1671 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 557 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
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