SG ID

SG00006964 

UniProt Accession

ANXA1_MOUSE;P10107;  

Theoretical PI

7.15  

Molecular Weight

38734 Da  

Genbank Nucleotide ID

X07486; M69260; M69250; M69251; M69252; M69253; M69254; M69255; M69256; M69257; M69258; M69259; BC002289; BC004594; M24554;  

Genbank Protein ID

CAA30371.1; AAA39437.1; AAA39437.1; AAA39437.1; AAA39437.1; AAA39437.1; AAA39437.1; AAA39437.1; AAA39437.1; AAA39437.1; AAA39437.1; AAA39437.1; AAH02289.1; AAH04594.1; AAA39420.1; 

Gene Name

Anxa1 

Gene Synonyms/Alias

Anx1, Lpc-1, Lpc1 

Protein Name

Annexin A1 

Protein Synonyms/Alias

Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35; 

Mus musculus (Mouse) 

10090 

Uncertain 

Uncertain 

0.153705771 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.

Temporarily unavailable 

Abstract of related literatures

1. PMID: [2974946] 

2. Using cDNA probes obtained from library screening and anchored polymerase chain reaction, we have isolated and characterized three overlapping mouse genomic clones that contain the mouse lipocortin I (Lipo I) structural gene. Restriction enzyme mapping, Southern blotting, and DNA sequencing were carried out on the cloned genomic DNA. Lipo I spans about 17 kb and is divided into 13 exons encoding a protein of 346 amino acid residues. The promoter region of the gene has a TATA box and a CCAAT box located upstream of the transcription initiation site at -31 and -76 bp, respectively. Analysis of the strain distribution pattern of Lipo 1 in BXD and AKXD collections of recombinant inbred strains establishes that Lipo I is located on chromosome 19 in close proximity to Ea-4. While no striking relationship exists between the exon/intron structure of the gene and the four repeated 70-amino-acid domains of the protein, three of the four 17-amino-acid repeats believed to be responsible for Ca2+/phospholipid binding are encoded by the last codon of one exon and the first 16 codons of the following exon. This pattern supports the gene duplication theory, and the similarity in gene structure between mouse Lipo I and Lipo II suggests they have a recent evolutionary ancestor. PMID: [1676980] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

4. We isolated and sequenced mouse lipocortin I cDNA clones from a lambda gt10 cDNA library prepared from Swiss 3T3 mRNA. The homology with human lipocortin I at the amino acid level is 86%. When confluent layers of Swiss 3T3 cells were stimulated with 10% fetal calf serum, expression of lipocortin I was strongly stimulated. In parallel, DNA synthesis was induced with a peak at 24 hours after glucocorticoid treatment indicating induction of cell proliferation. In the absence of serum glucocorticoid treatment provoked neither induction of DNA synthesis nor expression of lipocortin I. We conclude that serum contains an unidentified factor, which acts synergistically with glucocorticoids on cell proliferation and lipocortin I expression. PMID: [2522299] 

5. Mutations in the dysferlin gene cause limb girdle muscular dystrophy type 2B and Miyoshi myopathy. We report here the results of expression profile analyses and in vitro investigations that point to an interaction between dysferlin and the Ca2+ and lipid-binding proteins, annexins A1 and A2, and define a role for dysferlin in Ca2+-dependent repair of sarcolemmal injury through a process of vesicle fusion. Expression profiling identified a network of genes that are co-regulated in dysferlinopathic mice. Co-immunofluorescence, co-immunoprecipitation, and fluorescence lifetime imaging microscopy revealed that dysferlin normally associates with both annexins A1 and A2 in a Ca2+ and membrane injury-dependent manner. The distribution of the annexins and the efficiency of sarcolemmal wound-healing are significantly disrupted in dysferlin-deficient muscle. We propose a model of muscle membrane healing mediated by dysferlin that is relevant to both normal and dystrophic muscle and defines the annexins as potential muscular dystrophy genes. PMID: [14506282] 

6. We used on-line electron capture dissociation (ECD) for the large scale identification and localization of sites of phosphorylation. Each FT-ICR ECD event was paired with a linear ion trap collision-induced dissociation (CID) event, allowing a direct comparison of the relative merits of ECD and CID for phosphopeptide identification and site localization. Linear ion trap CID was shown to be most efficient for phosphopeptide identification, whereas FT-ICR ECD was superior for localization of sites of phosphorylation. The combination of confident CID and ECD identification and confident CID and ECD localization is particularly valuable in cases where a phosphopeptide is identified just once within a phosphoproteomics experiment. PMID: [19131326] 

Function

Calcium/phospholipid-binding protein which promotesmembrane fusion and is involved in exocytosis. This proteinregulates phospholipase A2 activity. It seems to bind from two tofour calcium ions with high affinity. 

Subcellular Location

Nucleus (By similarity). Cytoplasm (Bysimilarity). Cell projection, cilium (By similarity). Basolateralcell membrane (By similarity). Note=Found in the cilium, nucleusand basolateral cell membrane of ciliated cells in the trachealendothelium (By similarity). Found in the cytoplasm of type IIpneumocytes and alveolar macrophages (By similarity). 

Tissue Specificity

 

Gene Ontology

GO ID	GO term	Evidence
GO:0016323	C:basolateral plasma membrane	IEA:UniProtKB-SubCell.
GO:0005929	C:cilium	IEA:UniProtKB-SubCell.
GO:0001533	C:cornified envelope	IEA:Compara.
GO:0005615	C:extracellular space	IEA:Compara.
GO:0031966	C:mitochondrial membrane	IEA:Compara.
GO:0005634	C:nucleus	IEA:UniProtKB-SubCell.
GO:0043234	C:protein complex	IEA:Compara.
GO:0042383	C:sarcolemma	IDA:MGI.
GO:0005509	F:calcium ion binding	IEA:InterPro.
GO:0005544	F:calcium-dependent phospholipid binding	IEA:UniProtKB-KW.
GO:0019834	F:phospholipase A2 inhibitor activity	IEA:UniProtKB-KW.
GO:0003697	F:single-stranded DNA binding	IEA:Compara.
GO:0003727	F:single-stranded RNA binding	IEA:Compara.
GO:0005198	F:structural molecule activity	IEA:Compara.
GO:0046632	P:alpha-beta T cell differentiation	IMP:BHF-UCL.
GO:0050482	P:arachidonic acid secretion	IMP:MGI.
GO:0007049	P:cell cycle	IMP:MGI.
GO:0007166	P:cell surface receptor signaling pathway	IEA:Compara.
GO:0070301	P:cellular response to hydrogen peroxide	IEA:Compara.
GO:0031018	P:endocrine pancreas development	IEA:Compara.
GO:0060206	P:estrous cycle phase	IEA:Compara.
GO:0042063	P:gliogenesis	IEA:Compara.
GO:0070365	P:hepatocyte differentiation	IEA:Compara.
GO:0030073	P:insulin secretion	IEA:Compara.
GO:0030216	P:keratinocyte differentiation	IEA:Compara.
GO:0001776	P:leukocyte homeostasis	IMP:BHF-UCL.
GO:0002674	P:negative regulation of acute inflammatory response	IEA:Compara.
GO:0043066	P:negative regulation of apoptotic process	IEA:Compara.
GO:0050709	P:negative regulation of protein secretion	IEA:Compara.
GO:0018149	P:peptide cross-linking	IEA:Compara.
GO:2000427	P:positive regulation of apoptotic cell clearance	IMP:BHF-UCL.
GO:0043065	P:positive regulation of apoptotic process	IEA:Compara.
GO:0031394	P:positive regulation of prostaglandin biosynthetic process	IEA:Compara.
GO:0031340	P:positive regulation of vesicle fusion	IEA:Compara.
GO:0042127	P:regulation of cell proliferation	IMP:MGI.
GO:0042493	P:response to drug	IEA:Compara.
GO:0032355	P:response to estradiol stimulus	IEA:Compara.
GO:0051384	P:response to glucocorticoid stimulus	IEA:Compara.
GO:0070555	P:response to interleukin-1	IEA:Compara.
GO:0014070	P:response to organic cyclic compound	IEA:Compara.
GO:0043434	P:response to peptide hormone stimulus	IEA:Compara.
GO:0010165	P:response to X-ray	IEA:Compara.
GO:0007165	P:signal transduction	IMP:MGI.

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Interpro

IPR001464;    Annexin.
IPR018502;    Annexin_repeat.
IPR018252;    Annexin_repeat_CS.
IPR002388;    AnnexinI.
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Pfam

PF00191;    Annexin;    4.
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SMART

SM00335;    ANX;    4.
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PROSITE

PS00223;    ANNEXIN;    4.
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PRINTS

PR00196;    ANNEXIN.;   
PR00197;    ANNEXINI.;   
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Created Date

18-Oct-2012 

Record Type

GAS predicted 

Sequence Annotation

Nucleotide Sequence

Length: 1355 bp   Go to nucleotide: FASTA

Protein Sequence

Length: 346 bp   Go to amino acid: FASTA

The verified Protein-Protein interaction information

UniProt	Gene Symbol	Ref Databases
DYSF_MOUSE	Dysf	MINT
Q62406-1	_	IntAct
CSF1_MOUSE	Csf1	IntAct
DYSF_MOUSE	Dysf	MINT

Other Protein-Protein interaction resources

String database  

UniProt

Gene Symbol

Ref Databases

View Microarray data

Click to view expression 

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