SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00007510 
UniProt Accession
Theoretical PI
5.43  
Molecular Weight
59555 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Cct8 
Gene Synonyms/Alias
Cctq 
Protein Name
T-complex protein 1 subunit theta 
Protein Synonyms/Alias
TCP-1-theta CCT-theta; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:16;87483570-87496114;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.599150623 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. The chaperonin containing t-complex polypeptide 1 (TCP-1), as one of its subunits, CCT, is a cytosolic heterooligomeric molecular chaperone assisting in the folding of proteins in eukaryotic cytosol. We have isolated a Tcp-1-related 119-bp cDNA fragment from a human cDNA library by polymerase chain reaction, and cloned full-length mouse cDNAs orthologous to the human cDNA by hybridization. The nucleotide (nt) sequence of the longest mouse clone (1844 bp) shows an open reading frame (ORF) encoding a TCP-1-related polypeptide of 548 amino acids (aa) (59,562 Da). This gene is different from Tcp-1 and the six Tcp-1-related genes reported previously, Tcp-1 (Ccta), Cctb, Cctg, Cctd, Ccte, Cctz and Ccth, which encode subunits of CCT. The product of the novel gene was analysed using an antibody raised against the C terminus of the polypeptide deduced from the nt sequence. We found that this gene encodes a subunit of CCT (polypeptide S1; 62 kDa and pI 6.25 by two-dimensional gel analysis). We have named it Cctq, encoding the theta subunit of CCT (CCT theta). The aa sequence of CCT theta shows 23-29% identity to the other CCT subunits, alpha, beta, gamma, delta, epsilon, zeta and eta, and 29% identity to the archaebacterial chaperonin TF55. CCT theta also contains the motifs common to all the other subunits of CCT which are postulated to be involved in ATPase activity. PMID: [7890169] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
Molecular chaperone; assists the folding of proteinsupon ATP hydrolysis. As part of the BBS/CCT complex may play arole in the assembly of BBSome, a complex involved in ciliogenesisregulating transports vesicles to the cilia. Known to play a role,in vitro, in the folding of actin and tubulin (By similarity). 
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Subcellular Location
Cytoplasm (By similarity). Cytoplasm,cytoskeleton, centrosome (By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005815 C:microtubule organizing center IEA:UniProtKB-SubCell.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0006457 P:protein folding IEA:InterPro.
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Interpro
IPR012721;    Chap_CCT_theta.
IPR017998;    Chaperone_TCP-1.
IPR002194;    Chaperonin_TCP-1_CS.
IPR002423;    Cpn60/TCP-1.
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Pfam
PF00118;    Cpn60_TCP1;    1.
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SMART
PROSITE
PS00750;    TCP1_1;    1.
PS00751;    TCP1_2;    1.
PS00995;    TCP1_3;    1.
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PRINTS
PR00304;    TCOMPLEXTCP1.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
INIT_MET      1      1       Removed (By similarity).
CHAIN         2    548       T-complex protein 1 subunit theta.
                             /FTId=PRO_0000128374.
MOD_RES       2      2       N-acetylalanine (By similarity).
MOD_RES       7      7       N6-acetyllysine (By similarity).
MOD_RES      20     20       N6-acetyllysine (By similarity).
MOD_RES      23     23       Phosphoserine (By similarity).
MOD_RES      30     30       Phosphotyrosine (By similarity).
MOD_RES     227    227       Phosphothreonine (By similarity).
MOD_RES     232    232       Phosphothreonine (By similarity).
MOD_RES     233    233       Phosphoserine (By similarity).
MOD_RES     318    318       N6-acetyllysine (By similarity).
MOD_RES     400    400       N6-acetyllysine (By similarity).
MOD_RES     466    466       N6-acetyllysine (By similarity).
MOD_RES     505    505       Phosphotyrosine (By similarity).
CROSSLNK    225    225       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK    235    235       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
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Nucleotide Sequence
Length: 1844 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 548 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
YwhabIntAct 
ACTBBioGRID 
PS1TP5BP1BioGRID 
SETIntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
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