Tag Content
SG ID
SG00007703 
UniProt Accession
Theoretical PI
7.94  
Molecular Weight
45341 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Lancl1 
Gene Synonyms/Alias
Gpr69a 
Protein Name
LanC-like protein 1 
Protein Synonyms/Alias
40 kDa erythrocyte membrane protein;p40 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:1;67047091-67085446;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.70422689 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. We isolated by 5'- and 3'-RACE (rapid amplification of cDNA ends) clones from a murine brain cDNA library which encode a putative G-protein-coupled receptor. The composite nucleotide sequence revealed a coding region of 1197 nt; the deduced amino acid sequence of 399 amino acids showed 91.5% identity (95.7% similarity) when compared with the human homolog. An intron-like sequence, possibly involved in the regulation of expression, was found within the 5'-untranslated region. Northern blot analysis showed that the major 1.7-kb transcript is widely expressed, notably in brain and testis. In situ hybridization studies of tissue sections revealed high expression in neurons of the brain, epithelial cells of the lung, kidney and intestine, and in alveolar macrophages. PMID: [9714732] 

2. We have determined the organization and chromosome location of the human LANCL1 and mouse Lancl1 genes encoding LANCL1, the lanthionine synthetase component C (LanC)-like protein 1. LANCL1 is related to the bacterial LanC family which is involved in the biosynthesis of antimicrobial peptides. The human and mouse genes span 45 kb and 38 kb, respectively, each comprising ten exons. Within the potential promoter regions, several consensus sequences for ubiquitous and tissue-specific transcription factors are present, reflecting the expression data. The nucleotide sequence of the previously unknown mouse full-length transcript is also reported here. Fluorescence in situ hybridization analyses assigned the LANCL1 gene to human chromosome 2q34 and the Lancl1 gene to mouse chromosome 1, region C2-C5, in accordance with the known homology. PMID: [11474189] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

4. Proteomic experiments were performed to identify novel glutathione (GSH) binding proteins expressed in the mammalian central nervous system. Bovine brain lysate was affinity purified using an immobilized glutathione-Sepharose column. Proteins that bound the immobilized glutathione were eluted with free glutathione and identified by one- and two-dimensional electrophoresis coupled with mass spectrometric analysis of tryptic fragments. Major proteins purified by this technique were glutathione S-transferase-mu (GST-mu) and GST-pi and lanthionine synthase C-like protein-1 (LanCL1). LanCL1 is a mammalian homologue of a prokaryotic enzyme responsible for the synthesis of thioether (lanthionine) cross-links within nascent polypeptide chains, yielding macrocyclic proteins with potent microbicidal activity. An antibody against LanCL1 was generated and applied to immunochemical studies of spinal cord tissue from SOD1G93A transgenic mice, a model for amyotrophic lateral sclerosis (ALS), wherein LanCL1 expression was found to be increased at presymptomatic stages of the disease. These results indicate LanCL1 is a glutathione binding protein possibly significant to neurodegenerative disease. PMID: [17305318] 

Back to Top
Function
May play a role in EPS8 signaling. Binds glutathion (Bysimilarity). 
Back to Top
Subcellular Location
Cytoplasm (By similarity). Membrane;Peripheral membrane protein (By similarity). Nucleus (Bysimilarity). 
Tissue Specificity
Detected in spinal cord (at protein level).Ubiquitous. Strongly expressed in brain, testis, alveolarmacrophages and epithelial cells of the lung, kidney andintestine. 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IEA:UniProtKB-SubCell.
GO:0016020 C:membrane IEA:UniProtKB-SubCell.
GO:0005634 C:nucleus IEA:UniProtKB-SubCell.
GO:0003824 F:catalytic activity IEA:InterPro.
GO:0043295 F:glutathione binding ISS:UniProtKB.
GO:0008270 F:zinc ion binding ISS:UniProtKB.
GO:0008152 P:metabolic process IEA:GOC.
Back to Top
Interpro
IPR008928;    6-hairpin_glycosidase-like.
IPR012341;    6hp_glycosidase.
IPR007822;    LANC-like.
IPR020464;    LanC-like_prot_euk.
Back to Top
Pfam
PF05147;    LANC_like;    1.
Back to Top
SMART
PROSITE
PRINTS
PR01951;    LANCEUKARYTE.;   
PR01950;    LANCSUPER.;   
Back to Top
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
INIT_MET      1      1       Removed (By similarity).
CHAIN         2    399       LanC-like protein 1.
                             /FTId=PRO_0000191269.
REGION      364    367       Glutathione binding (By similarity).
METAL       276    276       Zinc (By similarity).
METAL       322    322       Zinc (By similarity).
METAL       323    323       Zinc (By similarity).
BINDING     317    317       Glutathione (By similarity).
MOD_RES       2      2       N-acetylalanine (By similarity).
MOD_RES     142    142       N6-acetyllysine (By similarity).
Back to Top
Nucleotide Sequence
Length: 1402 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 399 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Dlg4IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Comments