Tag Content
SG ID
SG00007791 
UniProt Accession
Theoretical PI
8.47  
Molecular Weight
40766 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Prss39 
Gene Synonyms/Alias
Tesp1 
Protein Name
Inactive serine protease 39 
Protein Synonyms/Alias
Inactive testicular serine protease 1;Flags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:1;34555275-34559907;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.701877659 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. To identify a novel candidate(s) for acrosomal proteins that act on the sperm/egg interaction, a DNA fragment was PCR-amplified from a cDNA library of acrosin-deficient mouse testis and then used as a probe to screen a mouse testis cDNA library. Complementary DNA clones encoding each of two similar but different serine proteases, TESP1 and TESP2, have been identified. The nucleotide sequences of these clones indicate that mouse TESP1 and TESP2 are initially synthesized as preproproteins of 367 and 366 amino acids, respectively. Comparison of the two TESP sequences with those of typical serine proteases suggests that each TESP zymogen is probably converted into a two-chain mature enzyme consisting of light and heavy chains covalently linked by a single pre-existing disulfide bond. The conversion may be accomplished by another protease(s) with a trypsin-like cleavage specificity, since it is unlikely that the mature TESP1 and TESP2 are capable of splitting the Lys-Ile bond between the light and heavy chains. Northern blot analysis of total cellular RNA demonstrates that the TESP1 and TESP2 genes are expressed only in the testis, and the transcripts are abundantly present in the haploid round spermatids. Moreover, immunocytochemical analysis of mouse cauda epididymal sperm using affinity-purified antibodies reveals that these two TESPs are both localized in the sperm acrosome and are released during the acrosome reaction induced by calcium ionophore A23187. These findings provide additional clues for elucidating the mechanisms involved in the sperm/egg interactions, including penetration of the zona pellucida by sperm. PMID: [9588171] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
May play an important role in the sperm/egg interaction;released during the acrosome reaction. 
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Subcellular Location
Cytoplasmic vesicle, secretory vesicle,acrosome. Secreted. 
Tissue Specificity
Expressed in testis. More specifically,abundantly expressed in the haploid round spermatid. 
Gene Ontology
GO IDGO termEvidence
GO:0001669 C:acrosomal vesicle IEA:UniProtKB-SubCell.
GO:0005576 C:extracellular region IEA:UniProtKB-SubCell.
GO:0004252 F:serine-type endopeptidase activity IEA:InterPro.
GO:0006508 P:proteolysis IEA:InterPro.
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Interpro
IPR009003;    Pept_cys/ser_Trypsin-like.
IPR018114;    Peptidase_S1/S6_AS.
IPR001254;    Peptidase_S1_S6.
IPR001314;    Peptidase_S1A.
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Pfam
PF00089;    Trypsin;    1.
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SMART
SM00020;    Tryp_SPc;    1.
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PROSITE
PS50240;    TRYPSIN_DOM;    1.
PS00134;    TRYPSIN_HIS;    1.
PS00135;    TRYPSIN_SER;    1.
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PRINTS
PR00722;    CHYMOTRYPSIN.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
SIGNAL        1     31       Potential.
CHAIN        32    367       Inactive serine protease 39.
                             /FTId=PRO_0000344981.
DOMAIN       68    312       Peptidase S1.
DISULFID     93    109       By similarity.
DISULFID    192    269       By similarity.
DISULFID    225    248       By similarity.
DISULFID    259    287       By similarity.
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Nucleotide Sequence
Length: 1271 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 367 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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