Tag Content
UniProt Accession
Theoretical PI
Molecular Weight
27000 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gene Synonyms/Alias
Protein Name
EF-hand domain-containing protein D1 
Protein Synonyms/Alias
EF-hand domain-containing protein 1; Swiprosin-2; 
Mus musculus (Mouse) 
NCBI Taxonomy ID
Chromosome Location
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Probability (GAS) of Function in Spermatogenesis
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Temporarily unavailable 
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

3. We attempted to isolate genes involved in neuronal differentiation from a cell line 2Y-3t newly established from a mouse cerebellum. 2Y-3t cells proliferate in serum-containing medium and differentiate into neurons in serum-free medium. We took a subtraction method to isolate genes differentially expressed in differentiated cells and 17 cDNA clones were isolated. Functions of 6 cDNA clones are unknown. No. 60 cDNA clone has 723 nucleotides encoding 240 amino acid residues. It contains two putative EF-hand motifs and a coiled-coil region at C terminal end. Expression of the clone was undetectable at embryonic stage and was increased in brain during development. In situ hybridization showed that the expression was observed predominantly in neurons, suggesting that the protein may play roles in the neuronal differentiation and function. PMID: [12270117] 

Back to Top
Back to Top
Subcellular Location
Tissue Specificity
Highest expression in testis, followed byovary, kidney, cerebrum, cerebellum, heart, liver, and spleen. Inthe cerebrum and cerebellum, undetectable at embryonic stages,expression increases after birth up to adult stage. In adult CNS,detected in neurons of the cerebellum, cerebrum and hippocampusformation, including dentate gyrus and Cornu Ammonis, but not inthe white matter. In the testis, expressed in spermatocytes, butnot in spermatogonia nor in interstitial cells. In ovary, foundpredominantly in mural granulosa cells and those of the cumulusoophorus. In kidney, expressed in collecting ducts, but not inglomeruli. Not detected in skeletal muscle. 
Gene Ontology
GO IDGO termEvidence
GO:0005743 C:mitochondrial inner membrane IDA:MGI.
GO:0005509 F:calcium ion binding IEA:InterPro.
GO:0031175 P:neuron projection development IMP:MGI.
Back to Top
IPR011992;    EF-hand-like_dom.
IPR018249;    EF_HAND_2.
IPR002048;    EF_hand_Ca-bd.
Back to Top
SM00054;    EFh;    2.
Back to Top
PS00018;    EF_HAND_1;    FALSE_NEG.
PS50222;    EF_HAND_2;    2.
Back to Top
Created Date
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    240       EF-hand domain-containing protein D1.
DOMAIN       91    126       EF-hand 1.
DOMAIN      127    162       EF-hand 2.
CA_BIND     104    115       1 (Potential).
CA_BIND     140    151       2 (Potential).
Back to Top
Nucleotide Sequence
Length: 1805 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 240 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data