Tag Content
SG ID
SG00008206 
UniProt Accession
Theoretical PI
7.52  
Molecular Weight
95541 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Odf2 
Gene Synonyms/Alias
Odf84 
Protein Name
Outer dense fiber protein 2 
Protein Synonyms/Alias
84 kDa outer dense fiber protein; Cenexin; Outer dense fiber of sperm tails protein 2; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:2;29744741-29787266;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.762636943 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. The outer dense fibers (ODF) of the mammalian sperm tail comprise a unique, specialized, and very prominent structure, consisting of nine fibers surrounding the axoneme. The ODF may play an important but as yet undefined role in sperm morphology, integrity and function. Study of the ODF is hampered by insufficient knowledge of their protein composition and the genetic regulation of their synthesis. We report here on the characterization of cDNAs encoding the Odf2 proteins of outer dense fibers of mouse sperm. We isolated two cDNA clones with variable 5' regions. Variability in sequence is restricted to specific regions in the N-terminal part of the encoded proteins, whereas the C-terminal part is highly conserved in Odf2 proteins both between species and within a species. This variability is confirmed at the protein level. The outer dense fibers could be detected immunologically in total sperm tails allowing a direct comparison of their length in relation to the length of the sperm tail. Odf2 transcripts could be demonstrated in testicular RNA and are restricted to germ cells. The start of transcription is in step 5 spermatids of tubular stage V and the RNA could be detected in the cytoplasm of differentiating spermatids in all subsequent tubular stages. PMID: [9740324] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The mouse (Mus musculus) is the premier animal model for understanding human disease and development. Here we show that a comprehensive understanding of mouse biology is only possible with the availability of a finished, high-quality genome assembly. The finished clone-based assembly of the mouse strain C57BL/6J reported here has over 175,000 fewer gaps and over 139 Mb more of novel sequence, compared with the earlier MGSCv3 draft genome assembly. In a comprehensive analysis of this revised genome sequence, we are now able to define 20,210 protein-coding genes, over a thousand more than predicted in the human genome (19,042 genes). In addition, we identified 439 long, non-protein-coding RNAs with evidence for transcribed orthologs in human. We analyzed the complex and repetitive landscape of 267 Mb of sequence that was missing or misassembled in the previously published assembly, and we provide insights into the reasons for its resistance to sequencing and assembly by whole-genome shotgun approaches. Duplicated regions within newly assembled sequence tend to be of more recent ancestry than duplicates in the published draft, correcting our initial understanding of recent evolution on the mouse lineage. These duplicates appear to be largely composed of sequence regions containing transposable elements and duplicated protein-coding genes; of these, some may be fixed in the mouse population, but at least 40% of segmentally duplicated sequences are copy number variable even among laboratory mouse strains. Mouse lineage-specific regions contain 3,767 genes drawn mainly from rapidly-changing gene families associated with reproductive functions. The finished mouse genome assembly, therefore, greatly improves our understanding of rodent-specific biology and allows the delineation of ancestral biological functions that are shared with human from derived functions that are not. PMID: [19468303] 

4. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

5. The study of mammalian sperm tail outer dense fibers (ODF), a structure of unknown function, is hampered by the insoluble nature of ODF proteins and the availability of only one cloned component, Odf27. We report here the first use of the Odf27 leucine zipper as bait in a yeast two-hybrid screen to isolate a novel testis-specific protein whose interaction with Odf27 depends critically on the Odf27 leucine zipper. We find that the novel gene, 111-450, encodes a product that localizes to ODF as determined by fluorescence microscopy and immunoelectron microscopy and that the gene 111-450 product is identical to the major ODF protein, Odf84. Interestingly, Odf84 contains two C-terminal leucine zippers, and we demonstrate that all leucine residues in the upstream leucine zipper are required for interaction with Odf27, demonstrating the strategic validity of our approach. The use of the yeast screening approach to isolate leucine zipper containing proteins should be useful in other systems, and our findings have implications for ODF structural models. PMID: [9045620] 

6. Outer dense fibre 2 (Odf2; also known as cenexin) was initially identified as a main component of the sperm tail cytoskeleton, but was later shown to be a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Here we show that Odf2 expression is suppressed in mouse F9 cells when both alleles of Odf2 genes are deleted. Unexpectedly, the cell cycle of Odf2(-/-) cells does not seem to be affected. Immunofluorescence and ultrathin-section electron microscopy reveals that in Odf2(-/-) cells, distal/subdistal appendages disappear from mother centrioles, making it difficult to distinguish mother from daughter centrioles. In Odf2(-/-) cells, however, the formation of primary cilia is completely suppressed, although approximately 25% of wild-type F9 cells are ciliated under the steady-state cell cycle. The loss of primary cilia in Odf2(-/-) F9 cells can be rescued by exogenous Odf2 expression. These findings indicate that Odf2 is indispensable for the formation of distal/subdistal appendages and the generation of primary cilia, but not for other cell-cycle-related centriolar functions. PMID: [15852003] 

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Function
Seems to be a major component of sperm tail outer densefibers (ODF). ODFs are filamentous structures located on theoutside of the axoneme in the midpiece and principal piece of themammalian sperm tail and may help to maintain the passive elasticstructures and elastic recoil of the sperm tail. May have amodulating influence on sperm motility. Functions as a generalscaffold protein that is specifically localized at thedistal/subdistal appendages of mother centrioles. Component of thecentrosome matrix required for the localization of PLK1 and NIN tothe centrosomes. Required for the formation and/or maintenance ofnormal CETN1 assembly (By similarity). 
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Subcellular Location
Cytoplasm, cytoskeleton, centrosome. Cellprojection, cilium. Cytoplasm, cytoskeleton, centrosome,centriole. Cytoplasm, cytoskeleton, spindle pole. Note=Localizedat the microtubule organizing centers in interphase and spindlepoles in mitosis. Localized at the distal/subdistal appendages ofmother centrioles. 
Tissue Specificity
Testis-specific (at protein level). Expressedin spermatids at tubular stage V of the spermatogenic cycle.Highly expressed in the cytoplasm of elongating spermatids(tubular stages X/XI). In step 14/15 spermatids of tubular stageIII/IV low expression detected. No expression detected in othertesticular cells as well as the early round of spermatids. 
Gene Ontology
GO IDGO termEvidence
GO:0005814 C:centriole IDA:MGI.
GO:0005929 C:cilium IEA:UniProtKB-SubCell.
GO:0019861 C:flagellum IDA:MGI.
GO:0005874 C:microtubule IEA:UniProtKB-KW.
GO:0000922 C:spindle pole IEA:UniProtKB-SubCell.
GO:0005200 F:structural constituent of cytoskeleton TAS:MGI.
GO:0030154 P:cell differentiation IEA:UniProtKB-KW.
GO:0007275 P:multicellular organismal development IEA:UniProtKB-KW.
GO:0007283 P:spermatogenesis IEA:UniProtKB-KW.
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Interpro
IPR026099;    Odf2-rel.
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Pfam
SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    830       Outer dense fiber protein 2.
                             /FTId=PRO_0000299458.
COILED      144    217       Potential.
COILED      245    423       Potential.
COILED      461    798       Potential.
VAR_SEQ       1     47       Missing (in isoform 7).
                             /FTId=VSP_027671.
VAR_SEQ       1     41       MSASSSGGSPRFPSCGKNGVTSLTQKKVLRTPCGAPSVTVT
                             -> MKDRSSTPPLHVHVDENTPVHVHIKKLPKPSAASSQ
                             (in isoform 2, isoform 3, isoform 4 and
                             isoform 5).
                             /FTId=VSP_027672.
VAR_SEQ      65     83       Missing (in isoform 2 and isoform 6).
                             /FTId=VSP_027673.
VAR_SEQ     281    281       E -> EK (in isoform 4).
                             /FTId=VSP_027674.
VAR_SEQ     638    657       IEHQGDKLEMAREKHQASQK -> VRDWQKGSHELARAGAR
                             LPR (in isoform 5, isoform 6 and isoform
                             7).
                             /FTId=VSP_027675.
VAR_SEQ     658    830       Missing (in isoform 5, isoform 6 and
                             isoform 7).
                             /FTId=VSP_027676.
CONFLICT    349    349       L -> W (in Ref. 2; AAH57001).
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Nucleotide Sequence
Length: 2220 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 830 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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