Tag Content
UniProt Accession
Theoretical PI
Molecular Weight
22662 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gene Synonyms/Alias
Protein Name
DnaJ homolog subfamily C member 5B 
Protein Synonyms/Alias
Cysteine string protein beta;CSP-beta 
Mus musculus (Mouse) 
NCBI Taxonomy ID
Chromosome Location
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Probability (GAS) of Function in Spermatogenesis
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Temporarily unavailable 
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

3. Cysteine string proteins (CSPs) belong to the DnaJ-like chaperone family and play an important role in regulated exocytosis in neurons and endocrine cells. The palmitoylation of several residues in a cysteine string domain may anchor CSPs to the exocytotic vesicle surface and in pancreatic beta-cells, Cspalpha is localized on insulin containing large dense core vesicles (LDCVs). An isoform closely related to Cspalpha, Cspbeta, has been obtained from testis cell cDNA libraries. To gain insights on this isoform and more generally on the properties of CSPs, we compared Cspalpha and Cspbeta. In pull-down experiments, Cspbeta was able to interact to the same extent with two of the known Cspalpha chaperone partners, Hsc70 and SGT. Upon transient overexpression in clonal beta-cells, Cspbeta but not Cspalpha was mainly produced as a non-palmitoylated protein and mutational analysis indicated that domains distinct from the cysteine string are responsible for this difference. As Cspbeta remained tightly bound to membranes, intrinsic properties of CSPs are sufficient for interactions with membranes. Indeed, recombinant Cspalpha and Cspbeta were capable to interact with membranes even in their non-palmitoylated forms. Furthermore, overexpressed Cspbeta was not associated with LDCVs, but was localized at the trans-Golgi network. Our results suggest a possible correlation between the specific membrane targeting and the palmitoylation level of CSPs. PMID: [17034881] 

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Subcellular Location
Membrane; Lipid-anchor (By similarity). 
Tissue Specificity
Gene Ontology
GO IDGO termEvidence
GO:0016020 C:membrane IEA:UniProtKB-SubCell.
GO:0006457 P:protein folding IEA:InterPro.
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IPR001623;    DnaJ_N.
IPR003095;    Hsp_DnaJ.
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PF00226;    DnaJ;    1.
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SM00271;    DnaJ;    1.
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PS00636;    DNAJ_1;    FALSE_NEG.
PS50076;    DNAJ_2;    1.
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PR00625;    JDOMAIN.;   
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Created Date
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    199       DnaJ homolog subfamily C member 5B.
DOMAIN       19     84       J.
COMPBIAS    123    140       Cys-rich.
COMPBIAS    126    137       Poly-Cys.
CONFLICT    111    111       K -> R (in Ref. 2; AAH49579).
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Nucleotide Sequence
Length: 911 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 199 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data