SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00008842 
UniProt Accession
Theoretical PI
6.29  
Molecular Weight
91710 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Ddx20 
Gene Synonyms/Alias
Dp103, Gemin3 
Protein Name
Probable ATP-dependent RNA helicase DDX20 
Protein Synonyms/Alias
EC=3.6.4.13 Component of gems 3; DEAD box protein 20; DEAD box protein DP 103; Gemin-3; Regulator of steroidogenic factor 1;ROSF-1 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:3;105481178-105490492;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.615295765 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. Spinal muscular atrophy is an autosomal recessive neurodegenerative disease of childhood, resulting from deletion or mutation of the survival motor neuron ( SMN ) gene on chromosome 5q13. SMN exists as part of a 300 kDa multi-protein complex, incorporating several proteins critically required in pre-mRNA splicing. Although SMN mutations render SMN defective in this role, the specific alpha-motor neuron degenerative phenotype seen in the disease remains unexplained. Here we demonstrate the isolation from mouse brain of the murine homologue of a recently identified novel RNA helicase of the DEAD box family, DP103, and its direct and specific binding of SMN. Previous work has shown that DP103 binds viral proteins known to interact with a cellular transcription factor to modulate gene expression. We suggest that the interaction between SMN and DP103 is further evidence for a role for SMN in transcriptional regulation and that SMN may be involved in the regulation of neuron-specific genes essential in neuronal development. PMID: [10767334] 

2. The nuclear receptor steroidogenic factor-1 (SF-1) is essential for development of the gonads, adrenal gland, and the ventromedial hypothalamic nucleus. It also regulates the expression of pivotal steroidogenic enzymes and other important proteins in the reproductive system. We sought to elucidate the mechanisms that govern the transcriptional activity of SF-1. We demonstrate here that a previously uncharacterized domain, located C-terminal to the DNA binding domain of SF-1, exhibits transcriptional repression function. Point mutations in this domain markedly potentiate the transcriptional activity of native SF-1. Using an SF-1 region that spans this proximal repression domain as bait in a yeast two-hybrid system, we cloned an SF-1 interacting protein that is homologous to human DP103, a member of the DEAD box family of putative RNA helicases. DP103 directly interacts with the proximal repression domain of SF-1, and mutations in this domain abrogate its interaction with DP103. DP103 is expressed predominantly in the testis and is also expressed at a lower level in other steroidogenic and nonsteroidogenic tissues. Functionally, DP103 exhibits a native transcriptional repression function that localizes to the C-terminal region of the protein and represses the activity of wild-type, but not mutant, SF-1. Together, the physical and functional interaction of DP103 with a previously unrecognized repression domain within SF-1 represents a novel mechanism for regulation of SF-1 activity. PMID: [11145740] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

4. Blepharophimosis-ptosis-epicanthus inversus syndrome type I is an autosomal disorder caused by mutations in FOXL2 gene and associated with premature ovarian failure in women by a dominant inheritance. FOXL2 is a recently identified protein that belongs to forkhead family transcription factor, of which signaling pathways are still unknown. Here, we show that FOXL2 induces apoptosis in both Chinese hamster ovary cells and rat granulosa cells, and it interacts with DP103, a DEAD box-containing protein. Overexpression of DP103 itself did not affect cell viability while its coexpression with FOXL2 led to the potentiation of cell death. Our results present previously undiscovered functions of these proteins, an apoptotic activity of FOXL2 in the ovary and a modulating activity of DP103 by interacting with FOXL2. PMID: [16153597] 

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Function
The SMN complex plays an essential role in spliceosomalsnRNP assembly in the cytoplasm and is required for pre-mRNAsplicing in the nucleus. It may also play a role in the metabolismof snoRNPs. 
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Subcellular Location
Cytoplasm. Nucleus, gem. Note=Localized insubnuclear structures next to coiled bodies, called Gemini ofCajal bodies (Gems). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0015030 C:Cajal body IEA:UniProtKB-SubCell.
GO:0005737 C:cytoplasm IEA:UniProtKB-SubCell.
GO:0005681 C:spliceosomal complex IEA:UniProtKB-KW.
GO:0017053 C:transcriptional repressor complex IDA:MGI.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0008026 F:ATP-dependent helicase activity IEA:InterPro.
GO:0003677 F:DNA binding IEA:UniProtKB-KW.
GO:0006917 P:induction of apoptosis IPI:MGI.
GO:0006397 P:mRNA processing IEA:UniProtKB-KW.
GO:0000122 P:negative regulation of transcription from RNA polymerase II promoter IDA:MGI.
GO:0008380 P:RNA splicing IEA:UniProtKB-KW.
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Interpro
IPR011545;    DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001;    Helicase_ATP-bd.
IPR001650;    Helicase_C.
IPR000629;    RNA-helicase_DEAD-box_CS.
IPR014014;    RNA_helicase_DEAD_Q_motif.
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Pfam
PF00270;    DEAD;    1.
PF00271;    Helicase_C;    1.
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SMART
SM00487;    DEXDc;    1.
SM00490;    HELICc;    1.
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PROSITE
PS00039;    DEAD_ATP_HELICASE;    1.
PS51192;    HELICASE_ATP_BIND_1;    1.
PS51194;    HELICASE_CTER;    1.
PS51195;    Q_MOTIF;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    825       Probable ATP-dependent RNA helicase
                             DDX20.
                             /FTId=PRO_0000055026.
DOMAIN       94    265       Helicase ATP-binding.
DOMAIN      300    449       Helicase C-terminal.
NP_BIND     107    114       ATP (By similarity).
NP_BIND     110    115       ATP (By similarity).
MOTIF        63     91       Q motif.
MOTIF       212    215       DEAD box.
BINDING      85     85       ATP; via carbonyl oxygen (By similarity).
BINDING      90     90       ATP (By similarity).
MOD_RES     188    188       Phosphoserine (By similarity).
MOD_RES     501    501       Phosphoserine (By similarity).
MOD_RES     552    552       Phosphothreonine (By similarity).
MOD_RES     650    650       Phosphoserine (By similarity).
MOD_RES     653    653       Phosphoserine (By similarity).
MOD_RES     655    655       Phosphoserine (By similarity).
MOD_RES     657    657       Phosphoserine (By similarity).
MOD_RES     673    673       Phosphoserine (By similarity).
MOD_RES     678    678       Phosphoserine (By similarity).
MOD_RES     679    679       Phosphoserine (By similarity).
MOD_RES     706    706       Phosphothreonine (By similarity).
CONFLICT      5      5       A -> T (in Ref. 2; AAF76301).
CONFLICT      9      9       P -> R (in Ref. 2; AAF76301).
CONFLICT    226    226       Q -> H (in Ref. 1; CAB86201).
CONFLICT    468    468       V -> I (in Ref. 2; AAF76301).
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Nucleotide Sequence
Length: 2795 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 825 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
CdiptString 
CdiptString 
FOXL2IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
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