SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00009074 
UniProt Accession
Theoretical PI
8.53  
Molecular Weight
39507 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Aldob 
Gene Synonyms/Alias
Aldo2 
Protein Name
Fructose-bisphosphate aldolase B 
Protein Synonyms/Alias
EC=4.1.2.13 Aldolase 2; Liver-type aldolase; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:4;49548867-49562418;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.126584658 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

2. Protein phosphorylation is a complex network of signaling and regulatory events that affects virtually every cellular process. Our understanding of the nature of this network as a whole remains limited, largely because of an array of technical challenges in the isolation and high-throughput sequencing of phosphorylated species. In the present work, we demonstrate that a combination of tandem phosphopeptide enrichment methods, high performance MS, and optimized database search/data filtering strategies is a powerful tool for surveying the phosphoproteome. Using our integrated analytical platform, we report the identification of 5,635 nonredundant phosphorylation sites from 2,328 proteins from mouse liver. From this list of sites, we extracted both novel and known motifs for specific Ser/Thr kinases including a "dipolar" motif. We also found that C-terminal phosphorylation was more frequent than at any other location and that the distribution of potential kinases for these sites was unique. Finally, we identified double phosphorylation motifs that may be involved in ordered phosphorylation. PMID: [17242355] 

Back to Top
Function
 
Back to Top
Subcellular Location
 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0034451 C:centriolar satellite IEA:Compara.
GO:0005829 C:cytosol IEA:Compara.
GO:0005764 C:lysosome IEA:Compara.
GO:0005792 C:microsome IEA:Compara.
GO:0005634 C:nucleus IEA:Compara.
GO:0048471 C:perinuclear region of cytoplasm IEA:Compara.
GO:0005886 C:plasma membrane IEA:Compara.
GO:0030867 C:rough endoplasmic reticulum membrane IEA:Compara.
GO:0030868 C:smooth endoplasmic reticulum membrane IEA:Compara.
GO:0070061 F:fructose binding IEA:Compara.
GO:0004332 F:fructose-bisphosphate aldolase activity IEA:EC.
GO:0031210 F:phosphatidylcholine binding IEA:Compara.
GO:0031668 P:cellular response to extracellular stimulus IEA:Compara.
GO:0032869 P:cellular response to insulin stimulus IEA:Compara.
GO:0030388 P:fructose 1,6-bisphosphate metabolic process IEA:Compara.
GO:0006000 P:fructose metabolic process IEA:Compara.
GO:0006096 P:glycolysis IEA:UniProtKB-KW.
GO:0001889 P:liver development IEA:Compara.
GO:0006116 P:NADH oxidation IEA:Compara.
GO:0032781 P:positive regulation of ATPase activity IEA:Compara.
GO:0043200 P:response to amino acid stimulus IEA:Compara.
GO:0051591 P:response to cAMP IEA:Compara.
GO:0046688 P:response to copper ion IEA:Compara.
GO:0042493 P:response to drug IEA:Compara.
GO:0009750 P:response to fructose stimulus IEA:Compara.
GO:0051384 P:response to glucocorticoid stimulus IEA:Compara.
GO:0070741 P:response to interleukin-6 IEA:Compara.
GO:0014070 P:response to organic cyclic compound IEA:Compara.
GO:0042594 P:response to starvation IEA:Compara.
GO:0010043 P:response to zinc ion IEA:Compara.
GO:0070072 P:vacuolar proton-transporting V-type ATPase complex assembly IEA:Compara.
Back to Top
Interpro
IPR000741;    Aldolase_I.
IPR013785;    Aldolase_TIM.
Back to Top
Pfam
PF00274;    Glycolytic;    1.
Back to Top
SMART
PROSITE
PS00158;    ALDOLASE_CLASS_I;    1.
Back to Top
PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
INIT_MET      1      1       Removed.
CHAIN         2    364       Fructose-bisphosphate aldolase B.
                             /FTId=PRO_0000216941.
ACT_SITE    188    188       Proton acceptor (By similarity).
ACT_SITE    230    230       Schiff-base intermediate with
                             dihydroxyacetone-P.
BINDING      56     56       Substrate.
BINDING     147    147       Substrate.
SITE        364    364       Necessary for preference for fructose
                             1,6-bisphosphate over fructose 1-
                             phosphate.
MOD_RES       2      2       N-acetylalanine.
MOD_RES      36     36       Phosphoserine.
CONFLICT     81     81       H -> Y (in Ref. 2; AAH34173).
CONFLICT    155    156       AD -> DH (in Ref. 1; AAL06323).
Back to Top
Nucleotide Sequence
Length: 4364 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 364 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
Comments