SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00009659 
UniProt Accession
Theoretical PI
7.12  
Molecular Weight
91007 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Dhx15 
Gene Synonyms/Alias
Ddx15, Deah9 
Protein Name
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 
Protein Synonyms/Alias
EC=3.6.4.13 DEAH box protein 15; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:5;52541448-52581758;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.566187403 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. Yeast splicing factor Prp43, a DEAH box protein of the putative RNA helicase/RNA-dependent NTPase family, is a splicing factor that functions late in the pre-mRNA splicing pathway to facilitate spliceosome disassembly. In this paper we report cDNA cloning and characterization of mDEAH9, an apparent mammalian homologue of Prp43. Amino acid sequence comparison revealed that the two proteins are approximately 65% identical over a 500-aa region spanning the central helicase domain and the C-terminal region. Expression of mDEAH9 in S. cerevisiae bearing a temperature-sensitive mutation in prp43 was sufficient to restore growth at the nonpermissive temperature. This functional complementation was specific, as mouse mDEAH9 failed to complement mutations in related splicing factor genes prp16 or prp22. Finally, double label immunofluorescence experiments performed with mammalian cells revealed colocalization of mDEAH9 and splicing factor SC35 in punctate nuclear speckles. Thus, the hypothesis that mDEAH9 represents the mammalian homologue of yeast Prp43 is supported by its high sequence homology, functional complementation, and colocalization with a known splicing factor in the nucleus. Our results provide additional support for the hypothesis that the spliceosomal machinery that mediates regulated, dynamic changes in conformation of pre-mRNA and snRNP RNAs has been highly conserved through evolution. PMID: [9342318] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
Pre-mRNA processing factor involved in disassembly ofspliceosomes after the release of mature mRNA (By similarity). 
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Subcellular Location
Nucleus. Nucleus, nucleolus (By similarity). 
Tissue Specificity
Ubiquitous. 
Gene Ontology
GO IDGO termEvidence
GO:0005730 C:nucleolus IEA:UniProtKB-SubCell.
GO:0005689 C:U12-type spliceosomal complex ISS:HGNC.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0008026 F:ATP-dependent helicase activity IEA:InterPro.
GO:0003676 F:nucleic acid binding IEA:InterPro.
GO:0006397 P:mRNA processing IEA:UniProtKB-KW.
GO:0008380 P:RNA splicing IEA:UniProtKB-KW.
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Interpro
IPR003593;    AAA+_ATPase.
IPR011545;    DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464;    DNA/RNA_helicase_DEAH_CS.
IPR011709;    DUF1605.
IPR007502;    Helicase-assoc_dom.
IPR014001;    Helicase_ATP-bd.
IPR001650;    Helicase_C.
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Pfam
PF00270;    DEAD;    1.
PF04408;    HA2;    1.
PF00271;    Helicase_C;    1.
PF07717;    OB_NTP_bind;    1.
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SMART
SM00382;    AAA;    1.
SM00487;    DEXDc;    1.
SM00847;    HA2;    1.
SM00490;    HELICc;    1.
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PROSITE
PS00690;    DEAH_ATP_HELICASE;    1.
PS51192;    HELICASE_ATP_BIND_1;    1.
PS51194;    HELICASE_CTER;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    795       Putative pre-mRNA-splicing factor ATP-
                             dependent RNA helicase DHX15.
                             /FTId=PRO_0000055140.
DOMAIN      147    313       Helicase ATP-binding.
DOMAIN      338    518       Helicase C-terminal.
NP_BIND     160    167       ATP (By similarity).
MOTIF       260    263       DEAH box.
COMPBIAS    346    349       Poly-Glu.
COMPBIAS    395    398       Poly-Gln.
COMPBIAS    403    406       Poly-Pro.
MOD_RES      15     15       Phosphoserine (By similarity).
MOD_RES     132    132       N6-acetyllysine (By similarity).
MOD_RES     476    476       Phosphothreonine (By similarity).
MOD_RES     488    488       N6-acetyllysine (By similarity).
CONFLICT    388    389       LY -> FS (in Ref. 1).
CONFLICT    391    393       TLP -> YTS (in Ref. 1).
CONFLICT    757    758       WL -> CW (in Ref. 1).
CONFLICT    759    795       Missing (in Ref. 1).
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Nucleotide Sequence
Length: 2877 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 795 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
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