Tag Content
SG ID
SG00010363 
UniProt Accession
Theoretical PI
5.92  
Molecular Weight
95556 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Akap3 
Gene Synonyms/Alias
Akap110 
Protein Name
A-kinase anchor protein 3 
Protein Synonyms/Alias
AKAP-3 A-kinase anchor protein 110 kDa;AKAP 110 Protein kinase A-anchoring protein 3;PRKA3 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:6;126803116-126824325;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.720389749 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. Agents that increase intracellular cAMP are potent stimulators of sperm motility. Anchoring inhibitor peptides, designed to disrupt the interaction of the cAMP-dependent protein kinase A (PKA) with A kinase-anchoring proteins (AKAPs), are potent inhibitors of sperm motility. These data suggest that PKA anchoring is a key biochemical mechanism controlling motility. We now report the isolation, identification, cloning, and characterization of AKAP110, the predominant AKAP detected in sperm lysates. AKAP110 cDNA was isolated and sequenced from mouse, bovine, and human testis libraries. Using truncated mutants, the RII-binding domain was identified. Alignment of the RII-binding domain on AKAP110 to those from other AKAPs reveals that AKAPs contain eight functionally conserved positions within an amphipathic helix structure that are responsible for RII interaction. Northern analysis of eight different tissues detected AKAP110 only in the testis, and in situ hybridization analysis detected AKAP110 only in round spermatids, suggesting that AKAP110 is a protein found only in male germ cells. Sperm cells contain both RI, located primarily in the acrosomal region of the head, and RII, located exclusively in the tail, regulatory subunits of PKA. Immunocytochemical analysis detected AKAP110 in the acrosomal region of the sperm head and along the entire length of the principal piece. These data suggest that AKAP110 shares compartments with both RI and RII isoforms of PKA and may function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction. PMID: [10319321] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

3. The cAMP-dependent protein kinase (PKA) is targeted to specific subcellular compartments through its interaction with A-kinase anchoring proteins (AKAPs). AKAPs contain an amphipathic helix domain that binds to the type II regulatory subunit of PKA (RII). Synthetic peptides containing this amphipathic helix domain bind to RII with high affinity and competitively inhibit the binding of PKA with AKAPs. Addition of these anchoring inhibitor peptides to spermatozoa inhibits motility (Vijayaraghavan, S., Goueli, S. A., Davey, M. P., and Carr, D. W. (1997) J. Biol. Chem. 272, 4747-4752). However, inhibition of the PKA catalytic activity does not mimic these peptides, suggesting that the peptides are disrupting the interaction of AKAP(s) with proteins other than PKA. Using the yeast two-hybrid system, we have now identified two sperm-specific human proteins that interact with the amphipathic helix region of AKAP110. These proteins, ropporin (a protein previously shown to interact with the Rho signaling pathway) and AKAP-associated sperm protein, are 39% identical to each other and share a strong sequence similarity with the conserved domain on the N terminus of RII that is involved in dimerization and AKAP binding. Mutation of conserved residues in ropporin or RII prevents binding to AKAP110. These data suggest that sperm contains several proteins that bind to AKAPs in a manner similar to RII and imply that AKAPs may have additional and perhaps unique functions in spermatozoa. PMID: [11278869] 

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Function
May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosomereaction (By similarity). 
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Subcellular Location
Cytoplasmic vesicle, secretory vesicle,acrosome. Note=Ribs of the fibrous sheath in the principal pieceof the sperm tail. Dorsal margin of the acrosomal segment. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0001669 C:acrosomal vesicle IEA:UniProtKB-SubCell.
GO:0009434 C:microtubule-based flagellum IDA:MGI.
GO:0006928 P:cellular component movement IMP:MGI.
GO:0008104 P:protein localization IMP:MGI.
GO:0007178 P:transmembrane receptor protein serine/threonine kinase signaling pathway IPI:MGI.
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Interpro
IPR020799;    AKAP_110.
IPR018292;    AKAP_110_C.
IPR018459;    RII_binding_1.
IPR008382;    SPHK1-interactor_AKAP_110.
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Pfam
PF05716;    AKAP_110;    1.
PF10522;    RII_binding_1;    1.
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SMART
SM00807;    AKAP_110;    1.
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PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    864       A-kinase anchor protein 3.
                             /FTId=PRO_0000064527.
REGION      125    138       PKA-RII subunit binding domain.
MOD_RES     206    206       Phosphoserine (By similarity).
MOD_RES     405    405       Phosphoserine (By similarity).
MOD_RES     406    406       Phosphotyrosine (By similarity).
CONFLICT    215    215       T -> M (in Ref. 1; AAC63369).
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Nucleotide Sequence
Length: 3103 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 864 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
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