SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00010395 
UniProt Accession
Theoretical PI
6.58  
Molecular Weight
43045 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Ckm 
Gene Synonyms/Alias
Ckmm 
Protein Name
Creatine kinase M-type 
Protein Synonyms/Alias
EC=2.7.3.2 Creatine kinase M chain; M-CK; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:7;19996443-20006932;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.070975431 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. The nucleotide sequence of cloned DNA corresponding to full-length mouse muscle creatine kinase mRNA has been determined. This 1415 base pair DNA sequence and the deduced 381 amino acid sequence of the protein have been compared to creatine kinase sequences from other vertebrate species and to invertebrate guanidino kinase sequences. These comparisons show that the vertebrate muscle creatine kinases constitute a remarkably conserved protein family with a unit evolutionary period of 30. The creatine kinases also retain marked sequence similarity with the more distantly related invertebrate guanidino kinases. A portion of the sequence, presumably part of the ATP binding site, shows similarity to other nucleotide binding proteins with diverse functions. Comparisons of the untranslated regions of the creatine kinase cDNA sequences show that the 5' untranslated regions are more highly conserved than are the 3' untranslated regions; this may point to some regulatory function in the 5' region. PMID: [3003372] 

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Function
Reversibly catalyzes the transfer of phosphate betweenATP and various phosphogens (e.g. creatine phosphate). Creatinekinase isoenzymes play a central role in energy transduction intissues with large, fluctuating energy demands, such as skeletalmuscle, heart, brain and spermatozoa. 
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Subcellular Location
Cytoplasm. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005737 C:cytoplasm IEA:UniProtKB-SubCell.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0004111 F:creatine kinase activity IDA:MGI.
GO:0046314 P:phosphocreatine biosynthetic process IDA:MGI.
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Interpro
IPR000749;    ATP-guanido_PTrfase.
IPR022415;    ATP-guanido_PTrfase_AS.
IPR022414;    ATP-guanido_PTrfase_cat.
IPR022413;    ATP-guanido_PTrfase_N.
IPR014746;    Gln_synth/guanido_kin_cat_dom.
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Pfam
PF00217;    ATP-gua_Ptrans;    1.
PF02807;    ATP-gua_PtransN;    1.
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SMART
PROSITE
PS00112;    PHOSPHAGEN_KINASE;    1.
PS51510;    PHOSPHAGEN_KINASE_C;    1.
PS51509;    PHOSPHAGEN_KINASE_N;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    381       Creatine kinase M-type.
                             /FTId=PRO_0000211976.
DOMAIN       11     98       Phosphagen kinase N-terminal.
DOMAIN      125    367       Phosphagen kinase C-terminal.
NP_BIND     128    132       ATP (By similarity).
NP_BIND     320    325       ATP (By similarity).
BINDING     191    191       ATP (By similarity).
BINDING     236    236       ATP (By similarity).
BINDING     292    292       ATP (By similarity).
BINDING     335    335       ATP (By similarity).
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Nucleotide Sequence
Length: 1415 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 381 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
PrkceIntAct 
Mef2aIntAct 
NfixIntAct 
TRIM63BioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
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