SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00010582 
UniProt Accession
Theoretical PI
5.13  
Molecular Weight
109425 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Atp2a1 
Gene Synonyms/Alias
 
Protein Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 
Protein Synonyms/Alias
SERCA1SR Ca(2+)-ATPase 1EC=3.6.3.8 Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:7;133589372-133606622;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.148843581 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
This magnesium-dependent enzyme catalyzes the hydrolysisof ATP coupled with the translocation of calcium from the cytosolto the sarcoplasmic reticulum lumen. Contributes to calciumsequestration involved in muscular excitation/contraction (Bysimilarity). 
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Subcellular Location
Endoplasmic reticulum membrane; Multi-passmembrane protein. Sarcoplasmic reticulum membrane; Multi-passmembrane protein. 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005789 C:endoplasmic reticulum membrane ISS:UniProtKB.
GO:0005793 C:endoplasmic reticulum-Golgi intermediate compartment ISS:UniProtKB.
GO:0031673 C:H zone ISS:UniProtKB.
GO:0031674 C:I band ISS:UniProtKB.
GO:0016021 C:integral to membrane IEA:UniProtKB-KW.
GO:0005792 C:microsome IEA:Compara.
GO:0048471 C:perinuclear region of cytoplasm ISS:UniProtKB.
GO:0016529 C:sarcoplasmic reticulum ISS:UniProtKB.
GO:0033017 C:sarcoplasmic reticulum membrane IEA:UniProtKB-SubCell.
GO:0005524 F:ATP binding ISS:UniProtKB.
GO:0005509 F:calcium ion binding ISS:UniProtKB.
GO:0005388 F:calcium-transporting ATPase activity ISS:UniProtKB.
GO:0008637 P:apoptotic mitochondrial changes IEA:Compara.
GO:0006754 P:ATP biosynthetic process IEA:InterPro.
GO:0070509 P:calcium ion import IEA:Compara.
GO:0032470 P:elevation of endoplasmic reticulum calcium ion concentration IEA:Compara.
GO:0051561 P:elevation of mitochondrial calcium ion concentration IEA:Compara.
GO:0070059 P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress IEA:Compara.
GO:0051659 P:maintenance of mitochondrion location IEA:Compara.
GO:0045988 P:negative regulation of striated muscle contraction ISS:UniProtKB.
GO:0031448 P:positive regulation of fast-twitch skeletal muscle fiber contraction ISS:UniProtKB.
GO:0032471 P:reduction of endoplasmic reticulum calcium ion concentration IEA:Compara.
GO:0090076 P:relaxation of skeletal muscle IEA:Compara.
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Interpro
IPR023306;    ATPase_cation_domN.
IPR008250;    ATPase_P-typ_ATPase-assoc-dom.
IPR005782;    ATPase_P-typ_Ca-transp.
IPR006068;    ATPase_P-typ_cation-transptr_C.
IPR004014;    ATPase_P-typ_cation-transptr_N.
IPR023300;    ATPase_P-typ_cyto_domA.
IPR023299;    ATPase_P-typ_cyto_domN.
IPR000695;    ATPase_P-typ_H-transp.
IPR001757;    ATPase_P-typ_ion-transptr.
IPR018303;    ATPase_P-typ_P_site.
IPR023298;    ATPase_P-typ_TM_dom.
IPR005834;    Dehalogen-like_hydro.
IPR023214;    HAD-like_dom.
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Pfam
PF00689;    Cation_ATPase_C;    1.
PF00690;    Cation_ATPase_N;    1.
PF00122;    E1-E2_ATPase;    1.
PF00702;    Hydrolase;    1.
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SMART
SM00831;    Cation_ATPase_N;    1.
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PROSITE
PS00154;    ATPASE_E1_E2;    1.
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PRINTS
PR00119;    CATATPASE.;   
PR00120;    HATPASE.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    994       Sarcoplasmic/endoplasmic reticulum
                             calcium ATPase 1.
                             /FTId=PRO_0000046188.
TOPO_DOM      1     48       Cytoplasmic (By similarity).
TRANSMEM     49     69       Helical; Name=1; (By similarity).
TOPO_DOM     70     89       Lumenal (By similarity).
TRANSMEM     90    110       Helical; Name=2; (By similarity).
TOPO_DOM    111    253       Cytoplasmic (By similarity).
TRANSMEM    254    273       Helical; Name=3; (By similarity).
TOPO_DOM    274    295       Lumenal (By similarity).
TRANSMEM    296    313       Helical; Name=4; (By similarity).
TOPO_DOM    314    757       Cytoplasmic (By similarity).
TRANSMEM    758    777       Helical; Name=5; (By similarity).
TOPO_DOM    778    787       Lumenal (By similarity).
TRANSMEM    788    808       Helical; Name=6; (By similarity).
TOPO_DOM    809    828       Cytoplasmic (By similarity).
TRANSMEM    829    851       Helical; Name=7; (By similarity).
TOPO_DOM    852    897       Lumenal (By similarity).
TRANSMEM    898    917       Helical; Name=8; (By similarity).
TOPO_DOM    918    930       Cytoplasmic (By similarity).
TRANSMEM    931    949       Helical; Name=9; (By similarity).
TOPO_DOM    950    964       Lumenal (By similarity).
TRANSMEM    965    985       Helical; Name=10; (By similarity).
TOPO_DOM    986    994       Cytoplasmic (By similarity).
REGION      370    400       Interacts with phospholamban 1 (By
                             similarity).
REGION      788    808       Interacts with phospholamban 2 (By
                             similarity).
ACT_SITE    351    351       4-aspartylphosphate intermediate (By
                             similarity).
METAL       304    304       Calcium 2; via carbonyl oxygen (By
                             similarity).
METAL       305    305       Calcium 2; via carbonyl oxygen (By
                             similarity).
METAL       307    307       Calcium 2; via carbonyl oxygen (By
                             similarity).
METAL       309    309       Calcium 2 (By similarity).
METAL       703    703       Magnesium (By similarity).
METAL       707    707       Magnesium (By similarity).
METAL       768    768       Calcium 1 (By similarity).
METAL       771    771       Calcium 1 (By similarity).
METAL       796    796       Calcium 2 (By similarity).
METAL       799    799       Calcium 1 (By similarity).
METAL       800    800       Calcium 1 (By similarity).
METAL       800    800       Calcium 2 (By similarity).
METAL       908    908       Calcium 1 (By similarity).
DISULFID    876    888       By similarity.
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Nucleotide Sequence
Length: 3471 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 994 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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