SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00010682 
UniProt Accession
Theoretical PI
7.58  
Molecular Weight
51318 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Hpx 
Gene Synonyms/Alias
Hpxn 
Protein Name
Hemopexin 
Protein Synonyms/Alias
Flags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:7;112740127-112748630;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.177146569 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

3. A procedure to map N-glycosylation sites is presented here. It can be applied to purified proteins as well as to highly complex mixtures. The method exploits deglycosylation by PNGase F in a diagonal, reverse-phase chromatographic setup. When applied to 10 microL of mouse serum, affinity-depleted for its three most abundant components, 117 known or predicted sites were mapped in addition to 10 novel sites. Several sites were detected on soluble membrane or receptor components. Our method furthermore senses the nature of glycan structures and can detect differential glycosylation on a given site. These properties--high sensitivity and dependence on glycan imprinting--can be exploited for glycan-biomarker analysis. PMID: [16944957] 

4. A comprehensive understanding of the mouse plasma proteome is important for studies using mouse models to identify protein markers of human disease. To enhance our analysis of the mouse plasma proteome, we have developed a method for isolating low-abundance proteins using a cysteine-containing glycopeptide strategy. This method involves two orthogonal affinity capture steps. First, glycoproteins are coupled to an azlactone copolymer gel using hydrazide chemistry and cysteine residues are then biotinylated. After trypsinization and extensive washing, tethered N-glycosylated tryptic peptides are released from the gel using PNGase F. Biotinylated cysteinyl-containing glycopeptides are then affinity selected using a monomeric avidin gel and analyzed by LC-MS/MS. We have applied the method to a proteome analysis of mouse plasma. In two independent analyses using 200 muL each of C57BL mouse plasma, 51 proteins were detected. Only 42 proteins were seen when the same plasma sample was analyzed by glycopeptides only. A total of 104 N-glycosylation sites were identified. Of these, 17 sites have hitherto not been annotated in the Swiss-Prot database whereas 48 were considered probable, potential, or by similarity - i.e., based on little or no experimental evidence. We show that analysis by cysteine-containing glycopeptides allows detection of low-abundance proteins such as the epidermal growth factor receptor, the Vitamin K-dependent protein Z, the hepatocyte growth factor activator, and the lymphatic endothelium-specific hyaluronan receptor as these proteins were not detected in the glycopeptide control analysis. PMID: [17330941] 

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Function
Binds heme and transports it to the liver for breakdownand iron recovery, after which the free hemopexin returns to thecirculation. 
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Subcellular Location
Secreted. 
Tissue Specificity
Expressed by the liver and secreted in plasma. 
Gene Ontology
GO IDGO termEvidence
GO:0005615 C:extracellular space IEA:Compara.
GO:0046872 F:metal ion binding IEA:UniProtKB-KW.
GO:0042168 P:heme metabolic process IMP:MGI.
GO:0020027 P:hemoglobin metabolic process IMP:MGI.
GO:0002925 P:positive regulation of humoral immune response mediated by circulating immunoglobulin IMP:MGI.
GO:0002639 P:positive regulation of immunoglobulin production IMP:MGI.
GO:0060335 P:positive regulation of interferon-gamma-mediated signaling pathway IMP:MGI.
GO:0042511 P:positive regulation of tyrosine phosphorylation of Stat1 protein IMP:MGI.
GO:0006810 P:transport IEA:UniProtKB-KW.
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Interpro
IPR000585;    Hemopexin/matrixin.
IPR018486;    Hemopexin/matrixin_CS.
IPR018487;    Hemopexin/matrixin_repeat.
IPR016358;    Hemopexin_chordata.
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Pfam
PF00045;    Hemopexin;    5.
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SMART
SM00120;    HX;    6.
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PROSITE
PS00024;    HEMOPEXIN;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
SIGNAL        1     23       Potential.
CHAIN        24    460       Hemopexin.
                             /FTId=PRO_0000021407.
DOMAIN       56     93       Hemopexin-like 1.
DOMAIN       97    140       Hemopexin-like 2.
DOMAIN      187    230       Hemopexin-like 3.
DOMAIN      261    304       Hemopexin-like 4.
DOMAIN      306    349       Hemopexin-like 5.
METAL        79     79       Iron (heme 1 axial ligand) (By
                             similarity).
METAL       149    149       Iron (heme 1 axial ligand) (By
                             similarity).
METAL       235    235       Iron (heme 2 axial ligand) (By
                             similarity).
METAL       291    291       Iron (heme 2 axial ligand) (By
                             similarity).
CARBOHYD     38     38       N-linked (GlcNAc...).
CARBOHYD     64     64       N-linked (GlcNAc...) (Potential).
CARBOHYD    186    186       N-linked (GlcNAc...).
CARBOHYD    240    240       N-linked (GlcNAc...) (Potential).
CARBOHYD    246    246       N-linked (GlcNAc...) (Potential).
CARBOHYD    419    419       N-linked (GlcNAc...) (Potential).
DISULFID     50    230       By similarity.
DISULFID    148    153       By similarity.
DISULFID    187    199       By similarity.
DISULFID    255    458       By similarity.
DISULFID    364    406       By similarity.
DISULFID    416    433       By similarity.
CONFLICT     30     30       N -> H (in Ref. 3; AAH11246/AAH19901).
CONFLICT    233    233       R -> Q (in Ref. 4; AAB49490).
CONFLICT    453    453       Missing (in Ref. 4; AAB49490).
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Nucleotide Sequence
Length: bp   Go to nucleotide: FASTA
Protein Sequence
Length: 460 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Kcnma1IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
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