Tag Content
SG ID
SG00010869 
UniProt Accession
Theoretical PI
10.16  
Molecular Weight
29598 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Rps4x 
Gene Synonyms/Alias
Rps4 
Protein Name
40S ribosomal protein S4, X isoform 
Protein Synonyms/Alias
 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:X;99380280-99384733;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
4.29E-05 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. The human RPS4X and RPS4Y genes, located on the X and Y chromosomes, appear to encode isoforms of ribosomal protein S4. Haploinsufficiency of these genes may contribute to the human phenotype known as Turner syndrome. Although RPS4X maps near the X-inactivation center, the gene is expressed on inactive human X chromosomes. We cloned Rps4, the mouse homolog of RPS4X. Exploiting allelic variation in Rps4, we examined transcription of the gene from active and inactive mouse X chromosomes in vivo, in female mice carrying an X-autosome translocation. We report that mouse Rps4, unlike human RPS4X, is subject to X inactivation. This finding may explain, at least in part, why the phenotypic consequences of X monosomy are less severe in mice than in humans. PMID: [1783379] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

4. RPS4Y, a Y-linked gene in humans, appears to encode an isoform of ribosomal protein S4. A homologous locus on the human X chromosome, RPS4X, lies close to the X-inactivation center but fails to undergo X-inactivation. We have isolated a genomic clone from the mouse Rps4 locus, the homolog of human RPS4X. We derived an intron probe that hybridizes to the functional Rps4 locus but does not cross-hybridize to related sequences elsewhere in the mouse genome. Genetic mapping utilizing interspecific mouse backcrosses and the intron-specific probe demonstrates that Rps4 maps close to the Phka locus on the mouse X chromosome and in the vicinity of the X-inactivation center. The gene order Ccg-1-Rps4/Phka-Xist-Pgk-1 is conserved between mouse and human. PMID: [1740345] 

Back to Top
Function
 
Back to Top
Subcellular Location
Cytoplasm (By similarity). Note=Localized incytoplasmic mRNP granules containing untranslated mRNAs (Bysimilarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005844 C:polysome IEA:Compara.
GO:0015935 C:small ribosomal subunit ISA:MGI.
GO:0019843 F:rRNA binding IEA:UniProtKB-KW.
GO:0003735 F:structural constituent of ribosome IEA:Compara.
GO:0007275 P:multicellular organismal development IEA:Compara.
GO:0008284 P:positive regulation of cell proliferation IEA:Compara.
GO:0045727 P:positive regulation of translation IEA:Compara.
GO:0006412 P:translation IEA:InterPro.
Back to Top
Interpro
IPR005824;    KOW.
IPR000876;    Ribosomal_S4e.
IPR013845;    Ribosomal_S4e_central.
IPR013843;    Ribosomal_S4e_N.
IPR018199;    Ribosomal_S4e_N_CS.
IPR002942;    S4_RNA-bd.
Back to Top
Pfam
PF00467;    KOW;    1.
PF00900;    Ribosomal_S4e;    1.
PF08071;    RS4NT;    1.
PF01479;    S4;    1.
Back to Top
SMART
SM00363;    S4;    1.
Back to Top
PROSITE
PS00528;    RIBOSOMAL_S4E;    1.
PS50889;    S4;    1.
Back to Top
PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
INIT_MET      1      1       Removed (By similarity).
CHAIN         2    263       40S ribosomal protein S4, X isoform.
                             /FTId=PRO_0000130809.
DOMAIN       42    104       S4 RNA-binding.
MOD_RES     120    120       N6-acetyllysine (By similarity).
MOD_RES     134    134       N6-acetyllysine (By similarity).
MOD_RES     247    247       Phosphothreonine (By similarity).
CONFLICT      9      9       L -> M (in Ref. 2; BAB29207).
Back to Top
Nucleotide Sequence
Length: 885 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 263 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
YwhabIntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
Comments