SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00011035 
UniProt Accession
Theoretical PI
7.62  
Molecular Weight
84534 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Adam5 
Gene Synonyms/Alias
Tmdc2 
Protein Name
Disintegrin and metalloproteinase domain-containing protein 5 
Protein Synonyms/Alias
Transmembrane metalloproteinase-like, disintegrin-like, and cysteine-rich protein II;tMDC IIFlags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:8;25837565-25934841;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.949246601 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. Fertilin alpha and beta, previously known as PH-30 alpha and beta, are two subunits of a guinea pig sperm integral membrane protein implicated in sperm-egg binding and fusion. They are derived from sequence-similar precursors which contain a metalloprotease-like and a disintegrin-like domain and which are related to a family of metalloprotease and disintegrin domain-containing snake venom proteins. We report here the cloning, sequencing, and characterization of mouse fertilin alpha and beta as well as five additional sequence-similar cDNAs from guinea pig and mouse testis. We name this gene family ADAM, for proteins containing A Disintegrin And Metalloprotease domain, and in honor of its dual origins in the fields of snakes and fertility. In situ hybridization demonstrated that, in testis, RNA encoding these ADAMs is expressed only in spermatogenic cells and that this expression is developmentally regulated. PCR analysis of mouse tissue cDNA showed that these ADAMs display different patterns of tissue distribution. Some ADAMs (e.g., fertilin alpha) have the consensus active-site sequence for a zinc-dependent metalloprotease in their metalloprotease-like domain. All have a disintegrin-like domain, which could bind integrins or other receptors. Some have sequences which may be active in membrane fusion. All encode potential membrane-spanning domains. Searches of sequence databases revealed that additional mammalian members of the ADAM gene family have been cloned from a variety of tissues. Thus, the ADAMs are a large, widely expressed, and developmentally regulated family of proteins with multiple potential functions in cell-cell and cell-matrix interactions. PMID: [7750654] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
This is a non catalytic metalloprotease-like protein.May play a role in sperm-egg fusion (By similarity). 
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Subcellular Location
Membrane; Single-pass type I membraneprotein (Potential). 
Tissue Specificity
Detected in brain, kidney, liver, lung, spleenand ovary. Highly expressed in testis. 
Gene Ontology
GO IDGO termEvidence
GO:0016021 C:integral to membrane IEA:UniProtKB-KW.
GO:0004222 F:metalloendopeptidase activity IEA:InterPro.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0006508 P:proteolysis IEA:InterPro.
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Interpro
IPR006586;    ADAM_Cys-rich.
IPR001762;    Blood-coag_inhib_Disintegrin.
IPR018358;    Disintegrin_CS.
IPR000742;    EG-like_dom.
IPR013032;    EGF-like_CS.
IPR024079;    MetalloPept_cat_dom.
IPR001590;    Peptidase_M12B.
IPR002870;    Peptidase_M12B_N.
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Pfam
PF08516;    ADAM_CR;    1.
PF00200;    Disintegrin;    1.
PF01562;    Pep_M12B_propep;    1.
PF01421;    Reprolysin;    1.
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SMART
SM00608;    ACR;    1.
SM00050;    DISIN;    1.
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PROSITE
PS50215;    ADAM_MEPRO;    1.
PS00427;    DISINTEGRIN_1;    1.
PS50214;    DISINTEGRIN_2;    1.
PS00022;    EGF_1;    FALSE_NEG.
PS01186;    EGF_2;    1.
PS50026;    EGF_3;    1.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
SIGNAL        1     16       Potential.
PROPEP       17    142       Potential.
                             /FTId=PRO_0000349301.
CHAIN       143    751       Disintegrin and metalloproteinase domain-
                             containing protein 5.
                             /FTId=PRO_0000349300.
TOPO_DOM     17    691       Extracellular (Potential).
TRANSMEM    692    712       Helical; (Potential).
TOPO_DOM    713    751       Cytoplasmic (Potential).
DOMAIN      185    378       Peptidase M12B.
DOMAIN      388    476       Disintegrin.
DOMAIN      623    657       EGF-like.
COMPBIAS      3     11       Poly-Leu.
DISULFID    291    373       By similarity.
DISULFID    332    357       By similarity.
DISULFID    334    339       By similarity.
DISULFID    448    468       By similarity.
DISULFID    627    639       By similarity.
DISULFID    633    645       By similarity.
DISULFID    647    656       By similarity.
VAR_SEQ     676    699       DGKSYKQQSHSNLKKNQLQLILYI -> APPTNQVLLPQKK
                             IIWINHEKLTF (in isoform 3).
                             /FTId=VSP_035334.
VAR_SEQ     676    695       DGKSYKQQSHSNLKKNQLQL -> AHLRTHQSHQTQKGFCT
                             SLL (in isoform 4).
                             /FTId=VSP_035335.
VAR_SEQ     696    751       Missing (in isoform 4).
                             /FTId=VSP_035336.
VAR_SEQ     700    751       Missing (in isoform 3).
                             /FTId=VSP_035337.
VAR_SEQ     749    751       TSH -> SSTDPPITPNPERILYVFALKKH (in
                             isoform 2).
                             /FTId=VSP_035338.
CONFLICT     26     26       Q -> P (in Ref. 1; AAA74923).
CONFLICT     29     29       V -> E (in Ref. 1; AAA74923).
CONFLICT    346    346       I -> K (in Ref. 2; BAE36383).
CONFLICT    457    458       FV -> LL (in Ref. 1; AAA74923).
CONFLICT    470    475       PNTYAR -> LTHMHA (in Ref. 1; AAA74923).
CONFLICT    501    501       S -> I (in Ref. 1; AAA74923).
CONFLICT    521    521       R -> T (in Ref. 1; AAA74923).
CONFLICT    596    597       TF -> NI (in Ref. 1; AAA74923).
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Nucleotide Sequence
Length: 2447 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 751 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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