Tag Content
SG ID
SG00011147 
UniProt Accession
Theoretical PI
8.85  
Molecular Weight
26240 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Nudt21 
Gene Synonyms/Alias
Cpsf5 
Protein Name
Cleavage and polyadenylation specificity factor subunit 5 
Protein Synonyms/Alias
Nucleoside diphosphate-linked moiety X motif 21;Nudix motif 21 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:8;96543303-96560939;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.753563182 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
Component of the cleavage factor Im (CFIm) complex thatplays a key role in pre-mRNA 3'-processing. Involved inassociation with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) sitecleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage andpolyadenylation RNA substrates. The homodimer mediatessimultaneous sequence-specific recognition of two 5'-UGUA-3'elements within the pre-mRNA. Binds to, but does not hydrolyzemono- and di-adenosine nucleotides. May have a role in mRNA export(By similarity). 
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Subcellular Location
Nucleus (By similarity). Note=In punctatesubnuclear structures localized adjacent to nuclear speckles,called paraspeckles (By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005813 C:centrosome IEA:Compara.
GO:0005849 C:mRNA cleavage factor complex ISS:UniProtKB.
GO:0042382 C:paraspeckles ISS:UniProtKB.
GO:0017091 F:AU-rich element binding ISS:UniProtKB.
GO:0016787 F:hydrolase activity IEA:InterPro.
GO:0003729 F:mRNA binding ISS:UniProtKB.
GO:0006378 P:mRNA polyadenylation ISS:UniProtKB.
GO:0051262 P:protein tetramerization IEA:Compara.
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Interpro
IPR016706;    Cleav_polyA_spec_factor_su5.
IPR000086;    NUDIX_hydrolase_dom.
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Pfam
PF13869;    NUDIX_2;    1.
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SMART
PROSITE
PS51462;    NUDIX;    1.
PS00893;    NUDIX_BOX;    FALSE_NEG.
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PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
INIT_MET      1      1       Removed (By similarity).
CHAIN         2    227       Cleavage and polyadenylation specificity
                             factor subunit 5.
                             /FTId=PRO_0000057151.
DOMAIN       76    201       Nudix hydrolase.
REGION        2    147       Necessary for RNA-binding (By
                             similarity).
REGION       81    160       Necessary for interactions with PAPOLA
                             and PABPN1 (By similarity).
REGION      102    104       Interaction with RNA (By similarity).
MOTIF       109    130       Nudix box.
SITE         55     55       Interaction with RNA (By similarity).
SITE         63     63       Interaction with RNA (By similarity).
SITE        208    208       Interaction with RNA (By similarity).
MOD_RES       2      2       N-acetylserine (By similarity).
MOD_RES      23     23       N6-acetyllysine (By similarity).
MOD_RES      29     29       N6-acetyllysine (By similarity).
MOD_RES      40     40       Phosphotyrosine (By similarity).
MOD_RES      56     56       N6-acetyllysine (By similarity).
MOD_RES      73     73       N6-acetyllysine (By similarity).
MOD_RES     189    189       N6-acetyllysine (By similarity).
CONFLICT    111    111       L -> F (in Ref. 1; BAE27154).
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Nucleotide Sequence
Length: 966 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 227 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
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