SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00011172 
UniProt Accession
Theoretical PI
4.65  
Molecular Weight
28468 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Tpm4 
Gene Synonyms/Alias
 
Protein Name
Tropomyosin alpha-4 chain 
Protein Synonyms/Alias
Tropomyosin-4; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:8;74658874-74677041;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.157130804 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
Binds to actin filaments in muscle and non-muscle cells.Plays a central role, in association with the troponin complex, inthe calcium dependent regulation of vertebrate striated musclecontraction. Smooth muscle contraction is regulated by interactionwith caldesmon. In non-muscle cells is implicated in stabilizingcytoskeleton actin filaments. Binds calcium (By similarity). 
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Subcellular Location
Cytoplasm, cytoskeleton (By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0030863 C:cortical cytoskeleton IDA:MGI.
GO:0002102 C:podosome IDA:MGI.
GO:0001725 C:stress fiber ISO:MGI.
GO:0046872 F:metal ion binding IEA:UniProtKB-KW.
GO:0006979 P:response to oxidative stress IEA:Compara.
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Interpro
IPR000533;    Tropomyosin.
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Pfam
PF00261;    Tropomyosin;    1.
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SMART
PROSITE
PS00326;    TROPOMYOSIN;    1.
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PRINTS
PR00194;    TROPOMYOSIN.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
INIT_MET      1      1       Removed (By similarity).
CHAIN         2    248       Tropomyosin alpha-4 chain.
                             /FTId=PRO_0000205636.
COILED        2    248       By similarity.
MOD_RES       2      2       N-acetylalanine (By similarity).
MOD_RES     125    125       N6-acetyllysine (By similarity).
MOD_RES     126    126       Phosphotyrosine (By similarity).
MOD_RES     177    177       N6-acetyllysine (By similarity).
MOD_RES     184    184       N6-acetyllysine (By similarity).
MOD_RES     215    215       N6-acetyllysine (By similarity).
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Nucleotide Sequence
Length: 2118 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 248 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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