Tag Content
UniProt Accession
Theoretical PI
Molecular Weight
76185 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gene Synonyms/Alias
Protein Name
E3 ubiquitin-protein ligase RNF139 
Protein Synonyms/Alias
EC=6.3.2.- RING finger protein 139; Translocation in renal carcinoma on chromosome 8 protein; 
Mus musculus (Mouse) 
NCBI Taxonomy ID
Chromosome Location
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Probability (GAS) of Function in Spermatogenesis
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Temporarily unavailable 
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

3. A system which consisted of multidimensional liquid chromatography (Yin-yang MDLC) coupled with mass spectrometry was used for the identification of peptides and phosphopeptides. The multidimensional liquid chromatography combines the strong-cation exchange (SCX), strong-anion exchange (SAX), and reverse-phase methods for the separation. Protein digests were first loaded on an SCX column. The flow-through peptides from SCX were collected and further loaded on an SAX column. Both columns were eluted by offline pH steps, and the collected fractions were identified by reverse-phase liquid chromatography tandem mass spectrometry. Comprehensive peptide identification was achieved by the Yin-yang MDLC-MS/MS for a 1 mg mouse liver. In total, 14 105 unique peptides were identified with high confidence, including 13 256 unmodified peptides and 849 phosphopeptides with 809 phosphorylated sites. The SCX and SAX in the Yin-Yang system displayed complementary features of binding and separation for peptides. When coupled with reverse-phase liquid chromatography mass spectrometry, the SAX-based method can detect more extremely acidic (pI < 4.0) and phosphorylated peptides, while the SCX-based method detects more relatively basic peptides (pI > 4.0). In total, 134 groups of phosphorylated peptide isoforms were obtained, with common peptide sequences but different phosphorylated states. This unbiased profiling of protein expression and phosphorylation provides a powerful approach to probe protein dynamics, without using any prefractionation and chemical derivation. PMID: [17203969] 

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E3-ubiquitin ligase; acts as a negative regulator of thecell proliferation through mechanisms involving G2/M arrest andcell death. Required for MHC class I ubiquitination in cellsexpressing the cytomegalovirus protein US2 before dislocation fromthe endoplasmic reticulum (ER). Affects SREBP processing byhindering the SREBP/SCAP complex translocation from the ER to theGolgi, thereby reducing SREBF2 target gene expression. Requiredfor INSIG1 ubiquitination. May be required for EIF3 complexubiquitination. May function as a signaling receptor (Bysimilarity). 
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Subcellular Location
Endoplasmic reticulum membrane; Multi-passmembrane protein. 
Tissue Specificity
Gene Ontology
GO IDGO termEvidence
GO:0005783 C:endoplasmic reticulum ISS:UniProtKB.
GO:0005789 C:endoplasmic reticulum membrane IEA:UniProtKB-SubCell.
GO:0016021 C:integral to membrane IEA:UniProtKB-KW.
GO:0004842 F:ubiquitin-protein ligase activity IEA:Compara.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0008285 P:negative regulation of cell proliferation IEA:Compara.
GO:0017148 P:negative regulation of translation IEA:Compara.
GO:0060628 P:regulation of ER to Golgi vesicle-mediated transport IEA:Compara.
GO:0070613 P:regulation of protein processing IEA:Compara.
GO:0031396 P:regulation of protein ubiquitination ISS:UniProtKB.
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IPR025754;    TRC8_N_dom.
IPR001841;    Znf_RING.
IPR013083;    Znf_RING/FYVE/PHD.
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PF13705;    TRC8_N;    1.
PF13639;    zf-RING_2;    1.
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SM00184;    RING;    1.
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PS00518;    ZF_RING_1;    FALSE_NEG.
PS50089;    ZF_RING_2;    1.
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Created Date
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    668       E3 ubiquitin-protein ligase RNF139.
TRANSMEM     51     71       Helical; (Potential).
TRANSMEM     85    105       Helical; (Potential).
TRANSMEM    125    145       Helical; (Potential).
TRANSMEM    154    174       Helical; (Potential).
TRANSMEM    178    198       Helical; (Potential).
TRANSMEM    293    313       Helical; (Potential).
TRANSMEM    323    343       Helical; (Potential).
TRANSMEM    356    376       Helical; (Potential).
TRANSMEM    390    410       Helical; (Potential).
TRANSMEM    420    440       Helical; (Potential).
TRANSMEM    470    490       Helical; (Potential).
ZN_FING     547    586       RING-type; atypical.
MOD_RES     627    627       Phosphoserine (By similarity).
MOD_RES     636    636       Phosphoserine (By similarity).
MOD_RES     637    637       Phosphothreonine (By similarity).
MOD_RES     667    667       Phosphothreonine.
CONFLICT    248    248       T -> A (in Ref. 1; BAC28327).
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Nucleotide Sequence
Length: 2745 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 668 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
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