SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00013623 
UniProt Accession
Theoretical PI
4.84  
Molecular Weight
53256 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Cpe 
Gene Synonyms/Alias
 
Protein Name
Carboxypeptidase E 
Protein Synonyms/Alias
CPEEC=3.4.17.10 Carboxypeptidase H;CPH Enkephalin convertase; Prohormone-processing carboxypeptidase;Flags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:8;67071355-67171837;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.61230791 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. Mice homozygous for the fat mutation develop obesity and hyperglycaemia that can be suppressed by treatment with exogenous insulin. The fat mutation maps to mouse chromosome 8, very close to the gene for carboxypeptidase E (Cpe), which encodes an enzyme (CPE) that processes prohormone intermediates such as proinsulin. We now demonstrate a defect in proinsulin processing associated with the virtual absence of CPE activity in extracts of fat/fat pancreatic islets and pituitaries. A single Ser202Pro mutation distinguishes the mutant Cpe allele, and abolishes enzymatic activity in vitro. Thus, the fat mutation represents the first demonstration of an obesity-diabetes syndrome elicited by a genetic defect in a prohormone processing pathway. PMID: [7663508] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
Removes residual C-terminal Arg or Lys remaining afterinitial endoprotease cleavage during prohormone processing.Processes proinsulin. 
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Subcellular Location
Cytoplasmic vesicle, secretory vesiclemembrane; Peripheral membrane protein (By similarity). Secreted(By similarity). Note=Associated with the secretory granulemembrane through direct binding to lipid rafts in intragranularconditions. This binding is Ca(2+)-dependent (By similarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005576 C:extracellular region IEA:UniProtKB-SubCell.
GO:0005794 C:Golgi apparatus IEA:Compara.
GO:0030658 C:transport vesicle membrane IEA:UniProtKB-SubCell.
GO:0004180 F:carboxypeptidase activity IMP:MGI.
GO:0004181 F:metallocarboxypeptidase activity IEA:InterPro.
GO:0008270 F:zinc ion binding IEA:InterPro.
GO:0003214 P:cardiac left ventricle morphogenesis IEA:Compara.
GO:0030070 P:insulin processing IMP:MGI.
GO:0072657 P:protein localization in membrane IEA:Compara.
GO:0006508 P:proteolysis IEA:UniProtKB-KW.
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Interpro
IPR008969;    CarboxyPept-like_regulatory.
IPR014766;    CarboxyPept_regulatory_dom.
IPR000834;    Peptidase_M14.
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Pfam
PF00246;    Peptidase_M14;    1.
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SMART
SM00631;    Zn_pept;    1.
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PROSITE
PS00132;    CARBOXYPEPT_ZN_1;    1.
PS00133;    CARBOXYPEPT_ZN_2;    1.
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PRINTS
PR00765;    CRBOXYPTASEA.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
SIGNAL        1     27       By similarity.
PROPEP       28     42       Activation peptide (By similarity).
                             /FTId=PRO_0000004386.
CHAIN        43    476       Carboxypeptidase E.
                             /FTId=PRO_0000004387.
ACT_SITE    342    342       Nucleophile (By similarity).
METAL       114    114       Zinc (By similarity).
METAL       117    117       Zinc (By similarity).
METAL       248    248       Zinc (By similarity).
CARBOHYD    139    139       N-linked (GlcNAc...) (Potential).
CARBOHYD    390    390       N-linked (GlcNAc...) (Potential).
VARIANT     244    244       S -> P (in hyperproinsulinemia obese fat/
                             fat mice; reduced activity).
CONFLICT     25     25       A -> R (in Ref. 1; CAA43550).
CONFLICT     81     81       R -> A (in Ref. 1; CAA43550).
CONFLICT    167    167       S -> W (in Ref. 1; CAA43550).
CONFLICT    171    171       E -> Q (in Ref. 1; CAA43550).
CONFLICT    301    301       R -> C (in Ref. 1; CAA43550).
CONFLICT    310    310       V -> D (in Ref. 1; CAA43550).
CONFLICT    334    334       S -> SS (in Ref. 1; CAA43550).
CONFLICT    344    344       S -> T (in Ref. 1; CAA43550).
CONFLICT    367    367       S -> N (in Ref. 1; CAA43550).
CONFLICT    388    388       I -> N (in Ref. 1; CAA43550).
CONFLICT    425    425       A -> D (in Ref. 1; CAA43550).
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Nucleotide Sequence
Length: 1992 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 476 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
AppIntAct 
PRKAA1IntAct 
Other Protein-Protein interaction resources
String database  
View Microarray data
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