Tag Content
SG ID
SG00015241 
UniProt Accession
Theoretical PI
7.16  
Molecular Weight
34952 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Capza3 
Gene Synonyms/Alias
Cappa3, Gsg3 
Protein Name
F-actin-capping protein subunit alpha-3 
Protein Synonyms/Alias
CapZ alpha-3; Germ cell-specific protein 3; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:6;139990046-139991310;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.753086301 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. We have cloned cDNAs involved in germ cell-specific expression. For this, a subtracted cDNA library was generated by subtracting cDNAs derived from supporting cells of mutant testis from wild-type testis cDNAs. Detailed analyses of mRNA expression revealed that the genes corresponding to the cloned cDNAs were exclusively expressed in testes and were developmentally controlled. PMID: [7957958] 

2. The Gsg3 gene which expresses specifically in haploid germ cells is a mouse testicular homolog of somatic cell type actin capping protein alpha (ACP alpha). We have obtained a mouse Gsg3 genomic clone using cDNA as a probe. Sequencing data showed that the Gsg3 gene was not interrupted by introns. The transcription initiation site of the gene was preceded not by a TATA box or GC rich promoter motifs, but by two consensus cAMP-response element (CRE) motifs at the putative position. Southern blotting analysis showed that Gsg3 is a single copy gene in the mouse, and conserved in mammals. Phylogenetic analysis showed that Gsg3 is a novel ACP alpha specific for haploid germ cells. PMID: [10524250] 

3. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

4. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
F-actin-capping proteins bind in a Ca(2+)-independentmanner to the fast growing ends of actin filaments (barbed end)thereby blocking the exchange of subunits at these ends. Unlikeother capping proteins (such as gelsolin and severin), theseproteins do not sever actin filaments. May play a role in themorphogenesis of spermatid. 
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Subcellular Location
 
Tissue Specificity
Exclusively expressed in the testis. 
Gene Ontology
GO IDGO termEvidence
GO:0001669 C:acrosomal vesicle IEA:Compara.
GO:0030863 C:cortical cytoskeleton IDA:MGI.
GO:0008290 C:F-actin capping protein complex IEA:InterPro.
GO:0016020 C:membrane IDA:MGI.
GO:0030036 P:actin cytoskeleton organization IEA:InterPro.
GO:0051693 P:actin filament capping IEA:UniProtKB-KW.
GO:0007286 P:spermatid development IEA:Compara.
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Interpro
IPR017865;    F-actin_cap_asu_CS.
IPR002189;    WASH_F-actin_cap_alpha.
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Pfam
PF01267;    F-actin_cap_A;    1.
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SMART
PROSITE
PS00748;    F_ACTIN_CAPPING_A_1;    1.
PS00749;    F_ACTIN_CAPPING_A_2;    1.
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PRINTS
PR00191;    FACTINCAPA.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    299       F-actin-capping protein subunit alpha-3.
                             /FTId=PRO_0000208632.
CONFLICT    117    117       L -> I (in Ref. 3; BAB30213).
CONFLICT    225    225       F -> Y (in Ref. 3; BAB30213).
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Nucleotide Sequence
Length: 1028 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 299 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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