SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00015378 
UniProt Accession
Theoretical PI
5.92  
Molecular Weight
52696 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Pnliprp1 
Gene Synonyms/Alias
 
Protein Name
Pancreatic lipase-related protein 1 
Protein Synonyms/Alias
PL-RP1Flags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:19;58803377-58818659;1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.155906441 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1.

PURPOSE:

Differential display analysis was used to look for gender differences in lacrimal gland gene expression. The expression of a female-specific mouse lacrimal gland mRNA that encoded pancreatic lipase-related protein 1 (PLRP1) was identified and characterized. PMID: [10235541] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

4. Pancreatic triglyceride lipase (PTL) and its cofactor, colipase, are required for efficient dietary triglyceride digestion. In addition to PTL, pancreatic acinar cells synthesize two pancreatic lipase-related proteins (PLRP1 and PLRP2), which have a high degree of sequence and structural homology with PTL. The lipase activity of PLRP2 has been confirmed, whereas no known triglyceride lipase activity has been detected with PLRP1 up to now. To explore the biological functions of PLRP1 in vivo, we generated Plrp1 knockout (KO) mice in our laboratory. Here we show that the Plrp1 KO mice displayed mature-onset obesity with increased fat mass, impaired glucose clearance and the resultant insulin resistance. When fed on high-fat (HF) diet, the Plrp1 KO mice exhibited an increased weight gain, fat mass and severe insulin resistance compared with wild-type mice. Pancreatic juice extracted from Plrp1 KO mice had greater ability to hydrolyze triglyceride than that from the wild-type littermates. We propose that PLRP1 may function as a metabolic inhibitor in vivo of PLT-colipase-mediated dietary triglyceride digestion and provides potential anti-obesity targets for developing new drugs. PMID: [21115337] 

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Function
May function as inhibitor of dietary triglyceridedigestion. Lacks detectable lipase activity (in vitro) (Bysimilarity). 
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Subcellular Location
Secreted. Note=Secreted in acinar cells. 
Tissue Specificity
Expressed in female, but not in male, lacrimalgland. Expressed in male and female sublingual gland and pancreas. 
Gene Ontology
GO IDGO termEvidence
GO:0005615 C:extracellular space IEA:Compara.
GO:0005509 F:calcium ion binding ISS:UniProtKB.
GO:0004806 F:triglyceride lipase activity IEA:InterPro.
GO:0006629 P:lipid metabolic process IEA:InterPro.
GO:0031016 P:pancreas development IEA:Compara.
GO:0051384 P:response to glucocorticoid stimulus IEA:Compara.
GO:0043434 P:response to peptide hormone stimulus IEA:Compara.
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Interpro
IPR000734;    Lipase.
IPR008976;    Lipase_LipOase.
IPR013818;    Lipase_N.
IPR002331;    Lipase_panc.
IPR001024;    LipOase_LH2.
IPR016272;    Lipoprotein_lipase_LIPH.
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Pfam
PF00151;    Lipase;    1.
PF01477;    PLAT;    1.
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SMART
SM00308;    LH2;    1.
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PROSITE
PS00120;    LIPASE_SER;    1.
PS50095;    PLAT;    1.
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PRINTS
PR00823;    PANCLIPASE.;   
PR00821;    TAGLIPASE.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
SIGNAL        1     17       Potential.
CHAIN        18    473       Pancreatic lipase-related protein 1.
                             /FTId=PRO_0000017791.
DOMAIN      356    467       PLAT.
ACT_SITE    171    171       Nucleophile (By similarity).
ACT_SITE    194    194       Charge relay system (By similarity).
ACT_SITE    281    281       Charge relay system (By similarity).
METAL       205    205       Calcium; via carbonyl oxygen (By
                             similarity).
METAL       208    208       Calcium; via carbonyl oxygen (By
                             similarity).
METAL       210    210       Calcium (By similarity).
METAL       213    213       Calcium (By similarity).
DISULFID     21     27       By similarity.
DISULFID    109    120       By similarity.
DISULFID    255    279       By similarity.
DISULFID    303    314       By similarity.
DISULFID    317    322       By similarity.
DISULFID    451    467       By similarity.
CONFLICT     59     59       Y -> H (in Ref. 3; AAH90985).
CONFLICT     97    115       IDKGEENWVVDMCKNMFQV -> RPTSWPPGPSSGNNAEYA
                             G (in Ref. 2; AAH68266).
CONFLICT    159    161       NYS -> KYY (in Ref. 2; AAH68266).
CONFLICT    159    159       N -> D (in Ref. 3; AAH90985).
CONFLICT    282    282       L -> P (in Ref. 2; AAH68266).
CONFLICT    351    351       K -> R (in Ref. 2; AAH68266).
CONFLICT    407    407       K -> E (in Ref. 2; AAH68266).
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Nucleotide Sequence
Length: 1620 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 473 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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