Tag Content
SG ID
SG00017289 
UniProt Accession
Theoretical PI
4.3  
Molecular Weight
11166 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Prm3 
Gene Synonyms/Alias
 
Protein Name
Protamine-3 
Protein Synonyms/Alias
Sperm protamine P3; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:16;10790600-10791006;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.920037646 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. Structural data are presented on the protamine gene cluster (PGC) of human, mouse, rat, and bull. By restriction mapping we demonstrate that the organization of the protamine cluster is conserved throughout all four species, i.e., the genes are situated in a head to tail arrangement in the order: protamine 1-protamine 2-transition protein 2. Further, we established the nucleotide sequence of the entire human PGC (25 kb in total) and the 3' portion of the rat protamine cluster (PRM2 and TNP2 genes and intergenic region). In addition, a 1 kb fragment of the bovine and murine protamine cluster, situated between PRM2 and TNP2, was sequenced. This fragment is conserved regarding sequence, position, and orientation in all species examined, and was classified as likely coding region by gene recognition program GRAIL. Using the rat fragment as a probe in RNA blots, we detected a testis-specific signal of about 0.5 kb. Finally, we demonstrate a high density of Alu elements, both full and fragmented copies, in the human PGC and discuss their localization with respect to evolutionary and functional aspects. PMID: [8720108] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
Protamines substitute for histones in the chromatin ofsperm during the haploid phase of spermatogenesis. They compactsperm DNA into a highly condensed, stable and inactive complex. 
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Subcellular Location
Nucleus. Chromosome. 
Tissue Specificity
Testis. 
Gene Ontology
GO IDGO termEvidence
GO:0000786 C:nucleosome IEA:UniProtKB-KW.
GO:0005634 C:nucleus IEA:UniProtKB-SubCell.
GO:0003677 F:DNA binding IEA:UniProtKB-KW.
GO:0030154 P:cell differentiation IEA:UniProtKB-KW.
GO:0030261 P:chromosome condensation IEA:UniProtKB-KW.
GO:0007275 P:multicellular organismal development IEA:UniProtKB-KW.
GO:0007283 P:spermatogenesis IEA:UniProtKB-KW.
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Interpro
IPR026077;    PRMP3.
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Pfam
SMART
PROSITE
PRINTS
Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    101       Protamine-3.
                             /FTId=PRO_0000106638.
COMPBIAS     45     66       Asp/Glu-rich (highly acidic).
COMPBIAS     74     77       Poly-Leu.
CONFLICT      6      6       A -> S (in Ref. 1; CAA87412).
CONFLICT     13     13       G -> EQ (in Ref. 1; CAA87412).
CONFLICT     21     21       S -> N (in Ref. 1; CAA87412).
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Nucleotide Sequence
Length: 13812 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 101 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data
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