SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00019050 
UniProt Accession
Theoretical PI
9.28  
Molecular Weight
28914 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Cela2a 
Gene Synonyms/Alias
Ela-2, Ela2, Ela2a 
Protein Name
Chymotrypsin-like elastase family member 2A 
Protein Synonyms/Alias
EC=3.4.21.71 Elastase-2; Elastase-2A;Flags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:4;141370877-141382075;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.135641733 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. Elastase II and trypsin mRNAs were cloned in form of their cDNAs from pancreas of strain A/J mice, and their complete nucleotide sequences were determined. The elastase II mRNA is 912 nucleotides long and encodes a protein of 271 amino acids. The cloned trypsin mRNA species is 814 nucleotides long and encodes a protein of 246 amino acids. The elastase II gene, which exists as a single copy in the haploid mouse genome, measures 11.2 kb from cap to poly(A) site and is interrupted by at least seven introns. Between 5 and 10 trypsin genes exist in the mouse genome. Five different trypsin genes, two of which are closely linked in a tail-to-tail manner, were studied in detail. They vary in size between 3.4 and 4.0kb, and all are interrupted by four introns. DNA sequence comparison of the elastase II, trypsin and Amy-2a alpha-amylase genes reveals a conserved 13 nucleotide motif in their 5'-flanking regions. The differential accumulation of the elastase II and trypsin mRNAs in the cytoplasm of the acinar pancreatic cell is regulated predominantly at the transcriptional level. PMID: [3641189] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Function
Acts upon elastin. 
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Subcellular Location
Secreted. 
Tissue Specificity
Pancreas. 
Gene Ontology
GO IDGO termEvidence
GO:0005576 C:extracellular region IEA:UniProtKB-SubCell.
GO:0004252 F:serine-type endopeptidase activity IEA:InterPro.
GO:0006508 P:proteolysis IEA:UniProtKB-KW.
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Interpro
IPR009003;    Pept_cys/ser_Trypsin-like.
IPR018114;    Peptidase_S1/S6_AS.
IPR001254;    Peptidase_S1_S6.
IPR001314;    Peptidase_S1A.
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Pfam
PF00089;    Trypsin;    1.
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SMART
SM00020;    Tryp_SPc;    1.
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PROSITE
PS50240;    TRYPSIN_DOM;    1.
PS00134;    TRYPSIN_HIS;    1.
PS00135;    TRYPSIN_SER;    1.
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PRINTS
PR00722;    CHYMOTRYPSIN.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
SIGNAL        1     16
PROPEP       17     30       Activation peptide.
                             /FTId=PRO_0000027687.
CHAIN        31    271       Chymotrypsin-like elastase family member
                             2A.
                             /FTId=PRO_0000027688.
DOMAIN       31    269       Peptidase S1.
ACT_SITE     75     75       Charge relay system (By similarity).
ACT_SITE    123    123       Charge relay system (By similarity).
ACT_SITE    218    218       Charge relay system (By similarity).
DISULFID     60     76       By similarity.
DISULFID    157    224       By similarity.
DISULFID    188    204       By similarity.
DISULFID    214    245       By similarity.
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Nucleotide Sequence
Length: 912 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 271 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
CdiptString 
CdiptString 
Other Protein-Protein interaction resources
String database  
View Microarray data
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