0.966703634 The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072]
2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334]
Accepts ubiquitin from the E1 complex and catalyzes itscovalent attachment to other proteins. Catalyzes 'Lys-11'-linkedpolyubiquitination. Acts as an essential factor of the anaphasepromoting complex/cyclosome (APC/C), a cell cycle-regulatedubiquitin ligase that controls progression through mitosis. Actsby specifically elongating 'Lys-11'-linked polyubiquitin chainsinitiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates,enhancing the degradation of APC/C substrates by the proteasomeand promoting mitotic exit. Also acts by elongating ubiquitinchains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it ishowever unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for theAPC/C in vivo. Also involved in ubiquitination and subsequentdegradation of VHL, resulting in an accumulation of HIF1A. Invitro able to promote polyubiquitination using all 7 ubiquitin Lysresidues, except 'Lys-48'-linked polyubiquitination.
CHAIN 1 223 Ubiquitin-conjugating enzyme E2 S. /FTId=PRO_0000082508. ACT_SITE 95 95 Glycyl thioester intermediate (By similarity). MOD_RES 1 1 N-acetylmethionine (By similarity). CONFLICT 213 213 K -> N (in Ref. 1; BAC25019). Back to Top