Tag Content
UniProt Accession
Theoretical PI
Molecular Weight
24183 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Gene Synonyms/Alias
Protein Name
Ubiquitin-conjugating enzyme E2 S 
Protein Synonyms/Alias
EC= Ubiquitin carrier protein S; Ubiquitin-conjugating enzyme E2-24 kDa; Ubiquitin-conjugating enzyme E2-EPF5; Ubiquitin-protein ligase S; 
Mus musculus (Mouse) 
NCBI Taxonomy ID
Chromosome Location
View in Ensembl genome browser  
Function in Stage
Function in Cell Type
Probability (GAS) of Function in Spermatogenesis
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Temporarily unavailable 
Abstract of related literatures
1. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

2. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

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Accepts ubiquitin from the E1 complex and catalyzes itscovalent attachment to other proteins. Catalyzes 'Lys-11'-linkedpolyubiquitination. Acts as an essential factor of the anaphasepromoting complex/cyclosome (APC/C), a cell cycle-regulatedubiquitin ligase that controls progression through mitosis. Actsby specifically elongating 'Lys-11'-linked polyubiquitin chainsinitiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates,enhancing the degradation of APC/C substrates by the proteasomeand promoting mitotic exit. Also acts by elongating ubiquitinchains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it ishowever unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for theAPC/C in vivo. Also involved in ubiquitination and subsequentdegradation of VHL, resulting in an accumulation of HIF1A. Invitro able to promote polyubiquitination using all 7 ubiquitin Lysresidues, except 'Lys-48'-linked polyubiquitination. 
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Subcellular Location
Tissue Specificity
Gene Ontology
GO IDGO termEvidence
GO:0005680 C:anaphase-promoting complex ISS:UniProtKB.
GO:0005524 F:ATP binding IEA:UniProtKB-KW.
GO:0004842 F:ubiquitin-protein ligase activity ISS:UniProtKB.
GO:0051488 P:activation of anaphase-promoting complex activity ISS:UniProtKB.
GO:0031145 P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process ISS:UniProtKB.
GO:0051301 P:cell division IEA:UniProtKB-KW.
GO:0010458 P:exit from mitosis ISS:UniProtKB.
GO:0010994 P:free ubiquitin chain polymerization ISS:UniProtKB.
GO:0070979 P:protein K11-linked ubiquitination ISS:UniProtKB.
GO:0044314 P:protein K27-linked ubiquitination ISS:UniProtKB.
GO:0035519 P:protein K29-linked ubiquitination ISS:UniProtKB.
GO:0085020 P:protein K6-linked ubiquitination ISS:UniProtKB.
GO:0070534 P:protein K63-linked ubiquitination ISS:UniProtKB.
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IPR000608;    UBQ-conjugat_E2.
IPR023313;    UBQ-conjugating_AS.
IPR016135;    UBQ-conjugating_enzyme/RWD.
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PF00179;    UQ_con;    1.
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PS00183;    UBIQUITIN_CONJUGAT_1;    1.
PS50127;    UBIQUITIN_CONJUGAT_2;    1.
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Created Date
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1    223       Ubiquitin-conjugating enzyme E2 S.
ACT_SITE     95     95       Glycyl thioester intermediate (By
MOD_RES       1      1       N-acetylmethionine (By similarity).
CONFLICT    213    213       K -> N (in Ref. 1; BAC25019).
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Nucleotide Sequence
Length: 1001 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 223 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
Gene Symbol Ref Databases
Other Protein-Protein interaction resources
String database  
View Microarray data