SpermatogenesisOnline 1.0

Tag Content
SG ID
SG00020923 
UniProt Accession
Theoretical PI
9.84  
Molecular Weight
6382 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Usmg5 
Gene Synonyms/Alias
Dapit 
Protein Name
Up-regulated during skeletal muscle growth protein 5 
Protein Synonyms/Alias
Diabetes-associated protein in insulin-sensitive tissues; 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:19;47157961-47165115;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.182189701 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

2. Acetylation of proteins on lysine residues is a dynamic posttranslational modification that is known to play a key role in regulating transcription and other DNA-dependent nuclear processes. However, the extent of this modification in diverse cellular proteins remains largely unknown, presenting a major bottleneck for lysine-acetylation biology. Here we report the first proteomic survey of this modification, identifying 388 acetylation sites in 195 proteins among proteins derived from HeLa cells and mouse liver mitochondria. In addition to regulators of chromatin-based cellular processes, nonnuclear localized proteins with diverse functions were identified. Most strikingly, acetyllysine was found in more than 20% of mitochondrial proteins, including many longevity regulators and metabolism enzymes. Our study reveals previously unappreciated roles for lysine acetylation in the regulation of diverse cellular pathways outside of the nucleus. The combined data sets offer a rich source for further characterization of the contribution of this modification to cellular physiology and human diseases. PMID: [16916647] 

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Function
Plays a critical role in maintaining the ATP synthasepopulation in mitochondria (By similarity). 
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Subcellular Location
Mitochondrion membrane; Single-pass membraneprotein (By similarity).-----------------------------------------------------------------------Copyrighted by the UniProt Consortium, see http://www.uniprot.org/termsDistributed under the Creative Commons Attribution-NoDerivs License----------------------------------------------------------------------- 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0016021 C:integral to membrane IEA:UniProtKB-KW.
GO:0005753 C:mitochondrial proton-transporting ATP synthase complex ISS:UniProtKB.
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Interpro
IPR009125;    DAPIT.
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Pfam
SMART
PROSITE
PRINTS
PR01821;    DAPIT.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
INIT_MET      1      1       Removed (By similarity).
CHAIN         2     58       Up-regulated during skeletal muscle
                             growth protein 5.
                             /FTId=PRO_0000231579.
TRANSMEM     23     45       Helical; (Potential).
MOD_RES      16     16       N6-acetyllysine.
MOD_RES      17     17       N6-acetyllysine.
CONFLICT      5      5       E -> K (in Ref. 1; CAC03620).
CONFLICT     27     27       R -> K (in Ref. 1; CAC03620).
CONFLICT     55     55       V -> A (in Ref. 1; CAC03620).
CONFLICT     58     58       T -> TMDFEMSDLTC (in Ref. 1; CAC03620).
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Nucleotide Sequence
Length: 438 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 58 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
UniProt
 Gene Symbol Ref Databases
SNCABioGRID 
Other Protein-Protein interaction resources
String database  
View Microarray data
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