Tag Content
SG ID
SG00022519 
UniProt Accession
Theoretical PI
10.32  
Molecular Weight
14728 Da  
Genbank Nucleotide ID
Genbank Protein ID
Gene Name
Uba52 
Gene Synonyms/Alias
Ubcep2 
Protein Name
Ubiquitin-60S ribosomal protein L40 Ubiquitin 60S ribosomal protein L40 
Protein Synonyms/Alias
Ubiquitin A-52 residue ribosomal protein fusion product 1;Contains:Contains: CEP52;Flags: Precursor 
Organism
Mus musculus (Mouse) 
NCBI Taxonomy ID
10090 
Chromosome Location
chr:8;73032162-73034700;-1
View in Ensembl genome browser  
Function in Stage
Uncertain 
Function in Cell Type
Uncertain 
Probability (GAS) of Function in Spermatogenesis
0.001446815 
The probability was calculated by GAS algorithm, ranging from 0 to 1. The closer it is to 1, the more possibly it functions in spermatogenesis.
Description
Temporarily unavailable 
Abstract of related literatures
1. We have isolated and sequenced a 500-bp cDNA clone which is the murine homologue of a ubiquitin/60S ribosomal fusion protein. The cDNA and the deduced amino acid sequence are highly conserved. Northern blotting demonstrates constitutive expression in a mouse renal epithelial cell line and in mouse kidneys. Upon stimulation with interferon-gamma or phorbol esters, the expression is not changed, contrasting with the stimulation seen for polyubiquitin. Expression of the 16-kD ubiquitin/60S protein is also demonstrated in mouse epithelial cells. PMID: [10495888] 

2. This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development. PMID: [16141072] 

3. The National Institutes of Health's Mammalian Gene Collection (MGC) project was designed to generate and sequence a publicly accessible cDNA resource containing a complete open reading frame (ORF) for every human and mouse gene. The project initially used a random strategy to select clones from a large number of cDNA libraries from diverse tissues. Candidate clones were chosen based on 5'-EST sequences, and then fully sequenced to high accuracy and analyzed by algorithms developed for this project. Currently, more than 11,000 human and 10,000 mouse genes are represented in MGC by at least one clone with a full ORF. The random selection approach is now reaching a saturation point, and a transition to protocols targeted at the missing transcripts is now required to complete the mouse and human collections. Comparison of the sequence of the MGC clones to reference genome sequences reveals that most cDNA clones are of very high sequence quality, although it is likely that some cDNAs may carry missense variants as a consequence of experimental artifact, such as PCR, cloning, or reverse transcriptase errors. Recently, a rat cDNA component was added to the project, and ongoing frog (Xenopus) and zebrafish (Danio) cDNA projects were expanded to take advantage of the high-throughput MGC pipeline. PMID: [15489334] 

4. Protein phosphorylation is a complex network of signaling and regulatory events that affects virtually every cellular process. Our understanding of the nature of this network as a whole remains limited, largely because of an array of technical challenges in the isolation and high-throughput sequencing of phosphorylated species. In the present work, we demonstrate that a combination of tandem phosphopeptide enrichment methods, high performance MS, and optimized database search/data filtering strategies is a powerful tool for surveying the phosphoproteome. Using our integrated analytical platform, we report the identification of 5,635 nonredundant phosphorylation sites from 2,328 proteins from mouse liver. From this list of sites, we extracted both novel and known motifs for specific Ser/Thr kinases including a "dipolar" motif. We also found that C-terminal phosphorylation was more frequent than at any other location and that the distribution of potential kinases for these sites was unique. Finally, we identified double phosphorylation motifs that may be involved in ordered phosphorylation. PMID: [17242355] 

5. Protein ubiquitination and protein phosphorylation are two fundamental regulatory post-translational modifications controlling intracellular signalling events. However, the ubiquitin system is vastly more complex compared with phosphorylation. This is due to the ability of ubiquitin to form polymers, i.e. ubiquitin chains, of at least eight different linkages. The linkage type of the ubiquitin chain determines whether a modified protein is degraded by the proteasome or serves to attract proteins to initiate signalling cascades or be internalized. The present review focuses on the emerging complexity of the ubiquitin system. I review what is known about individual chain types, and highlight recent advances that explain how the ubiquitin system achieves its intrinsic specificity. There is much to be learnt from the better-studied phosphorylation system, and many key regulatory mechanisms underlying control by protein phosphorylation may be similarly employed within the ubiquitin system. For example, ubiquitination may have important allosteric roles in protein regulation that are currently not appreciated. PMID: [19754430] 

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Function
Ribosomal protein L40 is a component of the 60S subunitof the ribosome. 
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Subcellular Location
60S ribosomal protein L40: Cytoplasm (Bysimilarity). 
Tissue Specificity
 
Gene Ontology
GO IDGO termEvidence
GO:0005829 C:cytosol TAS:Reactome.
GO:0022625 C:cytosolic large ribosomal subunit IEA:Compara.
GO:0005654 C:nucleoplasm TAS:Reactome.
GO:0003735 F:structural constituent of ribosome IEA:InterPro.
GO:0006412 P:translation IEA:InterPro.
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Interpro
IPR001975;    Ribosomal_L40e.
IPR000626;    Ubiquitin.
IPR019954;    Ubiquitin_CS.
IPR019956;    Ubiquitin_subgr.
IPR019955;    Ubiquitin_supergroup.
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Pfam
PF01020;    Ribosomal_L40e;    1.
PF00240;    ubiquitin;    1.
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SMART
SM00213;    UBQ;    1.
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PROSITE
PS00299;    UBIQUITIN_1;    1.
PS50053;    UBIQUITIN_2;    1.
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PRINTS
PR00348;    UBIQUITIN.;   
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Created Date
18-Oct-2012 
Record Type
GAS predicted 
Sequence Annotation
CHAIN         1     76       Ubiquitin.
                             /FTId=PRO_0000396435.
CHAIN        77    128       60S ribosomal protein L40.
                             /FTId=PRO_0000138752.
DOMAIN        1     76       Ubiquitin-like.
BINDING      54     54       Activating enzyme.
BINDING      72     72       Activating enzyme.
SITE         68     68       Essential for function.
MOD_RES       6      6       N6-acetyllysine (By similarity).
MOD_RES      48     48       N6-acetyllysine (By similarity).
MOD_RES      57     57       Phosphoserine.
MOD_RES      65     65       Phosphoserine (By similarity).
MOD_RES      98     98       N6,N6,N6-trimethyllysine (By similarity).
CROSSLNK      6      6       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK     11     11       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK     27     27       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK     29     29       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK     33     33       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK     48     48       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK     63     63       Glycyl lysine isopeptide (Lys-Gly)
                             (interchain with G-Cter in ubiquitin) (By
                             similarity).
CROSSLNK     76     76       Glycyl lysine isopeptide (Gly-Lys)
                             (interchain with K-? in acceptor
                             proteins).
STRAND        2      7
STRAND       12     16
HELIX        23     33
HELIX        38     40
STRAND       41     44
HELIX        57     59
STRAND       66     71
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Nucleotide Sequence
Length: 500 bp   Go to nucleotide: FASTA
Protein Sequence
Length: 128 bp   Go to amino acid: FASTA
The verified Protein-Protein interaction information
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