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miRD (pre-microRNA Detection)
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For candidate hairpin sequence(if the number of sequences < 300).
>example_1 UACACUGUGGAUCCGGUGAGGUAGUAGGUUGUAUAGUUUGGAAUAUUACCACCGGUGAACUAUGCAAUUUUCUACCUUACCGGAGACAGAACUCUUCGA >example_2 AUGCUUCCGGCCUGUUCCCUGAGACCUCAAGUGUGAGUGUACUAUUGAUGCUUCACACCUGGGCUCUCCGGGUACCAGGACGGUUUGAGCAGAU >example_3 AAAGUGACCGUACCGAGCUGCAUACUUCCUUACAUGCCCAUACUAUAUCAUAAAUGGAUAUGGAAUGUAAAGAAGUAUGUAGAACGGGGUGGUAGU >example_4 UAAACAGUAUACAGAAAGCCAUCAAAGCGGUGGUUGAUGUGUUGCAAAUUAUGACUUUCAUAUCACAGCCAGCUUUGAUGUGCUGCCUGUUGCACUGU
For candidate hairpin sequence(if the number of sequences > 300).
Select a file:
(*.fa)
Parameters selection
Strategy selected:
Strategy A (Single-stem) and Strategy A (Multi-stem)
Strategy B (Single-stem) OR Strategy A (Multi-stem)
Strategy fusion (Single-stem) and Strategy A (Multi-stem)
(
?
)
Minimal free energy
<
:
-18
-19
-20
-21
-22
-23
-24
-25
-26
-27
-28
-29
-30
-31
-32
-33
-34
-35
-36
-37
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-39
-40
The number of paired nucleic acids
>
:
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
The number of paired nucleic acids
The number of consecutive paired nucleic acids
>
:
The number of consecutive paired nucleic acids
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