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Name: |
Qinghua Shi
Professor of School of Life Sciences, University of Science and Technology of China
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Address: |
School of Life Sciences, University of Science and Technology of China,
443 Huangshan Road, Hefei, Anhui, P. R. China,
230026
tel:86-551-63600344/63600442(L)
fax: 86-551-63600344
e-mail: qshi@ustc.edu.cn
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Lab Website: |
http://mcg.ustc.edu.cn
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EDUCATION AND RESEARCH EXPERIENCE
December, 2004-present: Principal Investigator and Professor in Cell Biology. School of Life Science,
University of Science and Technology of China
January, 2002—December, 2004: Research Fellow. Institute of Chemistry and Cell Biology,
Department of Cell Biology, Harvard Medical School.
November, 1998—December, 2001: Postdoctoral Fellow. Department of Medical Genetics,
University of Calgary, Canada.
September, 1994–June, 1998: Graduate for Ph.D. degree.
GSF-National Research Center for Environment and Health, Germany, and Nanjing Normal University.
October, 1992--October, 1993: Guest Scientist.
GSF-National Research Center for Environment and Health, Germany.
November, 1988--September, 1992: Research Assistant.
Anhui Institute of Occupational Health.
September, 1985--October, 1988: Graduate for M.S. degree.
Kunming Institute of Zoology, Chinese Academy of Sciences.
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HONORS AND AWARDS
1) Tan Jiazhen Life Science Innovation Award (2009)
2) Wang Kuan Cheng Education Award (2009)
3) National Outstanding Young Scholars Foundation (2007)
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PROFESSIONAL MEMBERSHIP
1) Member, American Society of Human Genetics (2000.1-present)
2) Member, American Society of Cell Biology (2003.1-present)
3) Executive director, Chinese Society for Cell Biology (2015-2018)
4) President, Anhui Society for Cell Biology (2015-2020)
5) Vice President, Cell Structure and Function Branch, Chinese Society of Cell Biology (2013-2017)
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EDITORIALS FOR SCHOLARLY JOURNALS
1) Editorial Board, PLOS ONE (2011-)
2) Editorial Board, Chinese Bulletin of Life Sciences (2010-)
4) Editorial Board, Chinese Journal of Cell Biology (2013-)
5) Editorial Board, Chinese Journal of Healthy Birth & Child Care (2013-)
6) Editorial Board, Hereditas (Beijing) (2014-)
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RESEARCH INTERESTS
1) The generation and fates of aneuploid cells: Pathways and molecular mechanisms
Aneuploidy, the numerical alteration of chromosomes, occurs in more than 76% human tumors. We are trying to understand how normal diploid cells become aneuploid ones, e.g. via multipolar division or bipolar division with chromosome non-disjunction or lagging. To achieve this goal, single-cell pedigrees are created and analyzed by long-time live cell imaging for at least three generations, and chromosome composition of daughter cells is determined by fluorescence in situ hybridization using chromosome probes following live cell imaging. Using these approaches, we have previously demonstrated that chromosomal nondisjunction during bipolar mitosis yields tetraploid rather than aneuploid cells (Nature 437:1038-1042, 2005), and are now focusing on how chromosome mis-segregation prevents the completion of cytokinesis.
2) Primordial follicle assembly and recruitment: Molecular basis and mechanisms
The primordial follicles, oocytes surrounded by squamous granulosa cells, present in the neonate represent the total number of gametes available to a female throughout her reproductive life. The assembly of primordial follicles and the subsequent transition of the primordial follicles to the primary follicles, which is called primordial follicle recruitment, are critical processes in normal ovarian physiology and remain to be elucidated. The differentially expressed microRNAs, genes or proteins during these processes are detected by deep sequencing of transcriptome and protein mass spectrometry, and subjected to RNA interference-based functional screening in our laboratory. The positive hits will be studied using gene modified mouse models for functions in primordial follicle assembly and recruitment.
3) Meiotic checkpoints and their regulation: DNA damage on fates of spermatocytes and oocytes
To achieve accurate homologous chromosome pairing, synapsis, recombination and segregation, hundreds of programmed DNA double strand breaks (DSBs) must occur and then be repaired during the prophase of meiosis. Failure to repair the DSBs results in meiotic arrest at zygotene or pachytene stage in male and oocyte loss during primordial follicle assembly in female, indicating the presence of different cell cycle checkpoints between the sexes. We try to understand the regulation mechanisms of the checkpoints during spermatogenesis and oogenesis by using gene modified mice.
4) Identification of genetic mutations causing human male infertility by disrupting meiosis
Meiosis is an essential process of spermatogenesis, anomalies of which may cause spermatogenesis failure and consequently infertility. Hundreds of genes have been reported to cause infertility by disrupting meiosis after mutated in mice. However, only several of them have been detected mutated in infertile human males. No classification and low number of cases, as well as inefficient technology for the detection of mutants in patients are accused for the failure to find mutations causing meiotic abnormalities. We are trying to identify such mutations by genome-wide exon sequencing of thousands of patients with non-obstructive azoospermia classified according to their meiotic abnormalities.
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PUBLICATIONS (*, Corresponding author, IF=Impact factor 2017; NC=Times cited until May, 2018)
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2. Yin H, Ma H, Hussain S, Zhang H, Xie X, Jiang L, Jiang X, Iqbal F, Bukhari I, Jiang H, Ali A, Zhong L, Li T, Fan S, Zhang B, Gao J, Li Y, Nazish J, Khan T, Khan M, Zubair M, Hao Q, Fang H, Huang J, Huleihel M, Sha J, Pandita TK, Zhang Y, Shi Q*. A homozygous FANCM frameshift pathogenic variant causes male infertility. Genet Med. 2018 (IF=9.937)
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3. Abofoul-Azab M, AbuMadighem A, Lunenfeld E, Kapelushnik J, Shi Q, Pinkas H, Huleihel M. Development of Postmeiotic Cells In Vitro from Spermatogonial Cells of Prepubertal Cancer Patients. Stem Cells Dev. 2018 (IF=3.315)
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4. Jiang H, Gao Q, Zheng W, Yin S, Wang L, Zhong L, Ali A, Khan T, Hao Q, Fang H, Sun X, Xu P, Pandita TK, Jiang X, Shi Q*. MOF influences meiotic expansion of H2AX phosphorylation and spermatogenesis in mice. PLoS Genet. 14(5):e1007300, 2018 (IF=5.540)
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5. Khan M, Jabeen N, Khan T, Hussain HMJ, Ali A, Khan R, Jiang L, Li T, Tao Q, Zhang X, Yin H, Yu C, Jiang X, Shi Q*. The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice. Sci. Rep. 8(1):4975, 2018 (IF=4.122; NC=1)
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6. Shi B, Xue J, Zhou J, Kasowitz SD, Zhang Y, Liang G, Guan Y, Shi Q, Liu M, Sha J, Huang X, Wang PJ. MORC2B is essential for meiotic progression and fertility. PLoS Genet. 14(1):e1007175, 2018 (IF=5.540)
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7. Yin S, Jiang X, Jiang H, Gao Q, Wang F, Fan S, Khan T, Jabeen N, Khan M, Ali A, Xu P, Pandita TK, Fan HY, Zhang Y, Shi Q*. Histone acetyltransferase KAT8 is essential for mouse oocyte development by regulating reactive oxygen species levels. Development 144(12):2165-2174, 2017 (IF=5.413; NC=5)
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8. Jiang L, Li T, Zhang X, Zhang B, Yu C, Li Y, Fan S, Jiang X, Khan T, Hao Q, Xu P, Nadano D, Huleihel M, Lunenfeld E, Wang PJ, Zhang Y, Shi Q*. RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. Curr Biol. 27(10):1498-1505.e6, 2017 (IF=9.251; NC=3)
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9. Pan H, Zhang X, Jiang H, Jiang X, Wang L, Qi Q, Bi Y, Wang J, Shi Q*, and Li R*. Ndrg3 gene regulates DSB repair during meiosis through modulation the ERK signal pathway in the male germ cells. Sci. Rep. 7:44440, 2017 (IF=4.122; NC=2)
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10. Wan C, Gao J, Zhang H, Jiang X, Zang Q, Ban R, Zhang Y, Shi Q*. CPSS 2.0: a computational platform update for the analysis of small RNA sequencing data. Bioinformatics 33(20):3289-3291, 2017 (IF=5.481; NC=2)
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11. Zhang T, Lv L, Huang Y, Ren X, and Shi Q*. Chromosome nondisjunction during bipolar mitoses of binucleated intermediates promote aneuploidy formation along with multipolar mitoses rather than chromosome loss in micronuclei induced by asbestos. Oncotarget. 8(7):11030-11041, 2017 (IF=5.008)
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12. Liu C, Li M, Li T, Zhao H, Huang J, Wang Y, Gao Q, Yu Y*, and Shi Q*. ECAT1 is essential for human oocyte maturation and pre-implantation development of the resulting embryos. Sci. Rep. 6:38192, 2016 (IF=4.122)
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13. Zhang Y, Zang Q, Zhang H, Ban R, Yang Y, Iqbal F, Li A, and Shi Q*. DeAnnCNV: a tool for online detection and annotation of copy number variations from whole-exome sequencing data Nucleic Acids Res 44(W1):W166-175, 2016. (IF=11.561; NC=7)
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14. Zhang Y, Zang Q, Xu B, Zheng W, Ban R, Zhang H, Yang Y, Hao Q, Iqbal F, Li A and Shi Q*. IsomiR Bank: a research resource for tracking IsomiRs. Bioinformatics 32(13):2069-71. 2016. (IF=5.481; NC=9)
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15. Zhou Q, Wang M, Yuan Y, Wang X, Fu R, Wan H, Xie M, Liu M, Guo X, Zheng Y, Feng G, Shi Q, Zhao XY, Sha J, Zhou Q. Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro. Cell Stem Cell 18(3):330-340. 2016. (IF=23.290; NC=111)
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16. Wu C, Wang L, Iqbal F, Jiang X, Bukhari I, Guo T, Yin G, Cooke HJ, Cao Z, Jiang H and Shi Q* Preferential Y-Y pairing and synapsis and abnormal meiotic recombination in a 47,XYY man with non obstructive azoospermia. Mol Cytogenet. 9:9. 2016. (IF=1.167; NC=1)
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17. Sun R, Sun YC, Ge W, Tan H, Cheng SF, Yin S, Sun XF, Li L, Dyce P, Li J, Yang X and Shi QH* The crucial role of Activin A on the formation of primordial germ cell-like cells from skin-derived stem cells in vitro. Cell Cycle. 14(19):3016-29 2015. (IF=3.304; NC=5)
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18. Ge W, Ma HG, Cheng SF, Sun YC, Sun LL, Sun XF, Li L, Dyce P, Li J, Shi QH* and Shen W. Differentiation of early germ cells from human skin-derived stem cells without exogenous gene integration. Sci Rep. 5:13822, 2015. (IF=4.122; NC=17)
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19. Zhang Y, Yu Z, Ban R, Zhang H, Iqbal F, Zhao A, Li A and Shi Q*. DeAnnCNV: a tool for online detection and annotation of copy number variations from whole-exome sequencing data. Nucleic Acids Res. 43(W1):W289-94 2015. (IF=11.561; NC=7)
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20.Chu B, Zhong L, Dou S, Wang J, Li J, Wang M,, Shi Q* , Mei Y, Wu M. miRNA-181 regulates embryo implantation in mice through targeting leukemia inhibitory factor. Mol Cell Biol 7(1):12-22,2015. (IF=5.595; NC=24)
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21.Wang L, Xu Z, Iqbal F, Zhong L, Zhang Y, Wu C, Zhou G, Jiang H, Bukhari I, Cooke HJ, Shi Q*. Decreased XY recombination and disturbed meiotic prophase I progression in an infertile 48, XYY, +sSMC man. Chromosome Res 23(2):267-76,2015 (IF=2.909; NC=1)
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22. Jiang X, Ma T, Zhang Y, Zhang H, Yin S, Zheng W, Wang L, Wang Z, Khan M, Sheikh SW, Bukhari I, Iqbal F, Cooke HJ, Shi Q*. Specific Deletion of Cdh2 in Sertoli Cells Leads to Altered Meiotic Progression and Subfertility of Mice. Biology of Reproduction 92(3):1-12,2015 (IF=3.184; NC=11)
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24. Wang L, Iqbal F, Li G, Jiang X, Bukhari I, Jiang H, Yang Q, Zhong L, Zhang Y, Hua J, Cooke HJ, Shi Q*. Abnormal meiotic recombination with complex chromosomal rearrangement in an azoospermic man. Reprod Biomed Online. 30(6):651-8,2015.(IF=2.967; NC=6) |
25. Gao X, Ma W, Nie J, Zhang C, Zhang J, Yao G, Han J, Xu J, Hu B, Du Y, Shi Q,Yang Z, Huang X,Zhang Y. A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation. Cell Death Dis 2015 Jan 22;6:e1610.(IF=5.638; NC=13) |
26. Zheng Wang, Hao Yin, Yuanwei Zhang, Yukun Feng, Zhaofeng Yan, Xiaohua Jiang, Ihtisham Bukhari, Furhan Iqbal, Howard J Cooke, and Qinghua Shi*. miR-214-mediated downregulation of RNF8 induces chromosomal instability in ovarian cancer cells Cell Cycle 13(2):3519-3528,2014.(IF=3.304; NC=13) |
27. Xiaohua Jiang, Huan Zhang, Shi Yin, Yuanwei Zhang, Weimei Yang, Wei Zheng, Liu Wang,Zheng Wang, Ihtisham Bukhari, Howard J. Cooke1, Furhan Iqbal & Qinghua Shi*. Specific deficiency of Plzf paralog, Zbtb20, in Sertoli cells does not affect spermatogenesis and fertility in mice Sci Rep 4:7062,2014.(IF=4.122; NC=9) |
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31. Wang J, Qian J, Hu Y, Kong X, Chen H, Shi Q, Jiang L, Wu C, Zou W, Chen Y, Xu J, Fang JY. ArhGAP30 promotes p53 acetylation and function in colorectal cancer. Nat Commun. 2014, 5:4735.(IF=12.353; NC=23) |
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33. Huan Zhang, Xiaohua Jiang, Yuanwei Zhang, Bo Xu, Juan Hua, Tieliang Ma,Wei Zheng,Rui Sun, Wei Shen, Howard J Cooke, Qiaomei Hao, Jie Qiao and Qinghua Shi*. microRNA 376a regulates follicle assembly by targeting
Pcna in fetal and neonatal mouse ovaries
hybrid cells Reproduction 148(1):43-54,2014(IF=3.086; NC=34) |
34. Zheng Wang, Hao Yin, Lei Lv, Yingying Feng, Shaopeng Chen, Junting Liang, Yun Huang, Xiaohua Jiang, Hanwei Jiang,Ihtisham Bukhari, Lijun Wu, Howard J Cooke, and Qinghua Shi*. Unrepaired DNA damage facilitates elimination
of uniparental chromosomes in interspecific
hybrid cells Cell Cycle 13(8):1345-56,2014 (IF=3.304; NC=9) |
35. Yang Q,Hua J,Wang L,Xu B,Zhang H,Ye N,Zhang Z,Yu D,Cooke HJ,Zhang Y, Shi Q *. MicroRNA and piRNA Profiles in Normal Human Testis Detected by Next Generation Sequencing Plos One 8(6):e66809, 2013 (IF=2.766; NC=74) |
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38. Wang XX, Ying P, Diao F, Wang Q, Ye D, Jiang C, Shen N, Xu N, Chen WB, Lai SS, Jiang S, Miao XL, Feng J, Tao WW, Zhao NW, Yao B, Xu ZP, Sun HX, Li JM, Sha JH, Huang XX, Shi Q,Tang H, Gao X,Li CJ. Altered protein prenylation in Sertoli cells is associated with adult infertility resulting from childhood mumps infection. J Exp Med. 2013, 210(8):1559-74.(IF=10.790; NC=24) |
39. Lv L,Zhang T,Yi Q,Huang Y,Wang Z,Hou H,Zhang H,Zhang W,Hao Q,Guo Z,Cooke HJ, Shi Q*. Tetraploid cells from cytokinesis failure induce aneuploidy and spontaneous transformation of mouse. Cell Cycle 11(15), 2864-2875, 2012 (IF=3.304; NC=56) |
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43. Huang Y, Jiang L, Yi Q, Lv L, Wang Z, Zhao X, Zhong L, Jiang H, Rasool S, Hao Q, Guo Z, Cooke HJ, Fenech M, Shi Q*. Lagging chromosomes entrapped in micronuclei are not 'lost' by cells. Cell Res 22(5):932-5, 2012 Feb 21.(IF=15.393; NC=20) |
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46. Chen D, Zhang Y, Yi Q, Huang Y, Hou H, Zhang Y, Hao Q, Cooke HJ, Li L, Sun Q, Shi Q*. Regulation of Asymmetrical Cytokinesis by cAMP during Meiosis I in Mouse Oocytes. PLoS One 7(1):e29735, 2012 (IF=2.766 ;NC=10) |
47. Yi Q, Zhao X, Huang Y, Ma T, Zhang Y, Hou H, Cooke HJ, Yang DQ, Wu M, Shi Q*. p53 dependent centrosome clustering prevents multipolar mitosis in tetraploid cells. PLoS One 6(11):e27304, 2011 (IF=2.766; NC=26) |
48. Chen Q, Shi X, Rudolph C, Yu Y, Zhang D, Zhao X, Mai S, Wang G, Schlegelberger B, Shi Q*. Recurrent trisomy and Robertsonian translocation of chromosome 14 in murine iPS cell lines. Chromosome Res 19(7):857-68, 2011 (IF=2.909; NC=18) |
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51. Zhang Y, Yang Y, Zhang H, Jiang X, Xu B, Xue Y, Cao Y, Zhai Q, Zhai Y, Xu M, Cooke HJ, Shi Q*. Prediction of novel pre-microRNAs with high accuracy through boosting and SVM. Bioinformatics 27(10):1436-7, 2011 (IF=5.481; NC=33) |
52. Xu B, Hua J, Zhang Y, Jiang X, Zhang H, Ma T, Zheng W, Sun R, Shen W, Sha J, Cooke HJ, Shi Q*. Proliferating cell nuclear antigen (PCNA) regulates primordial follicle assembly by promoting apoptosis of oocytes in fetal and neonatal mouse ovaries. PLoS One 6(1):e16046, 2011 (IF=2.766; NC=70) |
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55. Xu W, Chen J, Chen H, Diao R, Fok K, Dong J, Sun T, Chen W, Yu M, Zhang X, Tsang L, Lau A, Shi Q, Huang P, Chan H. Defective CFTR-dependent CREB activation results in impaired spermatogenesis and azoospermia. PLoS One. 2011, 6: e19120. ( IF=2.766; NC=58) |
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59. Song L, Li J, Liao W, Feng Y, Yu C, Hu L, Kong Q, Xu L, Zhang X, Liu W, Li M, Zhang L, Kang T, Fu L, Huang W, Xia Y, Tsao S, Li M, Band V, Band H, Shi Q, Zeng Y, Zeng M. The polycomb group protein Bmi-1 represses the tumor suppressor PTEN and induces epithelial-mesenchymal transition in human nasopharyngeal epithelial cells. Journal of Clinical Investigation 2009, 119:3626-36. (IF=13.251; NC=361) |
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63. Wang N, Zhang P, Guo X, Xie J, Huo R, Wang F, Chen L, Shen J, Zhou Z, Shi Q*, Zhao B, Sha J. Comparative proteome profile of immature rat ovary during primordial follicle assembly and development.Proteomics 2009, 9: 3425-3434. (IF=3.532; NC=13) |
64. Dong H, Li L, Song Z, Tang J, Xuc B, Zhai X, Sun L, Zhang P, Li Z, Pan Q, Shi Q*, Shen W. Premeiotic fetal murine germ cells cultured in vitro form typical oocyte-like cells but do not progress through meiosis. Theriogenology 2009 72: 219–231. (IF=2.136; NC=20) |
65. Rao X, Zhang Y, Yi Q, Hou H, Xu B, Chu L, Huang Y, Zhang W, Fenech M, Shi Q* . Multiple origins of spontaneously arising micronuclei in HeLa cells: direct evidence from long-term live cell imaging. Mutat Res 646(1-2):41-9, 2008 (IF=2.398; NC=55) |
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